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The Effect of Oxytocin on Social and Non-Social Behaviour and Striatal Protein Expression in C57BL/6N Mice.

Zhang X, Li Q, Zhang M, Lam S, Sham PC, Bu B, Chua SE, Wang W, McAlonan GM - PLoS ONE (2015)

Bottom Line: However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes.With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics.Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), 1095 Jiefang Ave., Wuhan, 430030, P.R. China.

ABSTRACT
Oxytocin has been suggested as a promising new treatment for neurodevelopmental disorders. However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes. Here we investigated the effects of a range of oxytocin doses on social and non-social behaviours in C57BL/6N mice of both sexes. As the striatum modulates social and non-social behaviours, and is implicated in neurodevelopmental disorders, we also conducted a pilot exploration of changes in striatal protein expression elicited by oxytocin. Oxytocin increased prepulse inhibition of startle but attenuated the recognition memory in male C57BL/6N mice. It increased social interaction time and suppressed the amphetamine locomotor response in both sexes. The striatum proteome following oxytocin exposure could be clearly discriminated from saline controls. With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics. The proteins affected by oxytocin could be broadly categorized as those that modulate glutamatergic, GABAergic or dopaminergic signalling and those that mediate cytoskeleton dynamics. Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

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Related in: MedlinePlus

Open field and Amphetamine test.Oxytocin doses: 10 μg/kg, 100 μg/kg and 1000 μg/kg. The subject explored the arena for 1 hour while drug free. Then the subject explored the same arena for another 30min after drug (saline or oxytocin) injection. Finally the subject explored the same arena again for 1hour after amphetamine injection.
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pone.0145638.g002: Open field and Amphetamine test.Oxytocin doses: 10 μg/kg, 100 μg/kg and 1000 μg/kg. The subject explored the arena for 1 hour while drug free. Then the subject explored the same arena for another 30min after drug (saline or oxytocin) injection. Finally the subject explored the same arena again for 1hour after amphetamine injection.

Mentions: The open field exploration test was tested following methods previously published [34]. It was performed in 40×40 cm2 rectangular white arenas. At the beginning of the session, the mouse was gently placed in the centre of the arena, and allowed to explore for 1hour while drug free. The mouse was then briefly removed from the arena, and drug administered quickly via the subcutaneous (s.c.) route. The arena was cleaned with 70% ethanol; the mouse was placed back in the arena and allowed to explore for another 30min. The mouse was again briefly removed from the arena and injected with 2.5 mg/kg d-amphetamine (calculated as the salt, sigma-Aldrich, Switzerland) dissolved in a 0.9% NaCl solution via the intraperitoneal route. The volume of injection was 5 ml/kg. The arena was cleaned again. The mouse was placed back in the arena and allowed to explore for another 1hour (Fig 2).


The Effect of Oxytocin on Social and Non-Social Behaviour and Striatal Protein Expression in C57BL/6N Mice.

Zhang X, Li Q, Zhang M, Lam S, Sham PC, Bu B, Chua SE, Wang W, McAlonan GM - PLoS ONE (2015)

Open field and Amphetamine test.Oxytocin doses: 10 μg/kg, 100 μg/kg and 1000 μg/kg. The subject explored the arena for 1 hour while drug free. Then the subject explored the same arena for another 30min after drug (saline or oxytocin) injection. Finally the subject explored the same arena again for 1hour after amphetamine injection.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696826&req=5

pone.0145638.g002: Open field and Amphetamine test.Oxytocin doses: 10 μg/kg, 100 μg/kg and 1000 μg/kg. The subject explored the arena for 1 hour while drug free. Then the subject explored the same arena for another 30min after drug (saline or oxytocin) injection. Finally the subject explored the same arena again for 1hour after amphetamine injection.
Mentions: The open field exploration test was tested following methods previously published [34]. It was performed in 40×40 cm2 rectangular white arenas. At the beginning of the session, the mouse was gently placed in the centre of the arena, and allowed to explore for 1hour while drug free. The mouse was then briefly removed from the arena, and drug administered quickly via the subcutaneous (s.c.) route. The arena was cleaned with 70% ethanol; the mouse was placed back in the arena and allowed to explore for another 30min. The mouse was again briefly removed from the arena and injected with 2.5 mg/kg d-amphetamine (calculated as the salt, sigma-Aldrich, Switzerland) dissolved in a 0.9% NaCl solution via the intraperitoneal route. The volume of injection was 5 ml/kg. The arena was cleaned again. The mouse was placed back in the arena and allowed to explore for another 1hour (Fig 2).

Bottom Line: However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes.With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics.Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (HUST), 1095 Jiefang Ave., Wuhan, 430030, P.R. China.

ABSTRACT
Oxytocin has been suggested as a promising new treatment for neurodevelopmental disorders. However, important gaps remain in our understanding of its mode of action, in particular, to what extent oxytocin modulates social and non-social behaviours and whether its effects are generalizable across both sexes. Here we investigated the effects of a range of oxytocin doses on social and non-social behaviours in C57BL/6N mice of both sexes. As the striatum modulates social and non-social behaviours, and is implicated in neurodevelopmental disorders, we also conducted a pilot exploration of changes in striatal protein expression elicited by oxytocin. Oxytocin increased prepulse inhibition of startle but attenuated the recognition memory in male C57BL/6N mice. It increased social interaction time and suppressed the amphetamine locomotor response in both sexes. The striatum proteome following oxytocin exposure could be clearly discriminated from saline controls. With the caveat that these results are preliminary, oxytocin appeared to alter individual protein expression in directions similar to conventional anti-psychotics. The proteins affected by oxytocin could be broadly categorized as those that modulate glutamatergic, GABAergic or dopaminergic signalling and those that mediate cytoskeleton dynamics. Our results here encourage further research into the clinical application of this peptide hormone, which may potentially extend treatment options across a spectrum of neurodevelopmental conditions.

Show MeSH
Related in: MedlinePlus