Limits...
Dysfunction of Liver Receptor Homolog-1 in Decidua: Possible Relevance to the Pathogenesis of Preeclampsia.

Zhang D, Cheng D, Liu T, Zhang Y, Chen ZJ, Zhang C - PLoS ONE (2015)

Bottom Line: However, the results of this study indicated that insufficient decidualization plays a significant role.However, the levels of NR5A1 mRNA and protein did not significantly differ between groups.The expression of NR5A2 was upregulated after in vitro decidualization, but the expression of NR5A1 remained low and showed no difference compared with that of the control cells.

View Article: PubMed Central - PubMed

Affiliation: Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Preeclampsia (PE) is a multisystem disorder unique to Homo sapiens that is known to cause maternal and perinatal mortality and morbidity. Between 5-7% of all pregnancies are affected by PE and it is responsible for approximately 50,000 maternal deaths annually. The pathogenesis of PE remains poorly understood. However, the results of this study indicated that insufficient decidualization plays a significant role. NR5A1 and NR5A2 are orphan members of the Ftz-F1 subfamily of nuclear receptors and are involved in mammal follicular development, female reproduction, steroidogenesis, and decidualization. The expression of NR5A1 and NR5A2 in the human decidua and their functions during decidualization were investigated using in vitro cultured cells by real-time PCR, immunohistochemistry, western blotting, and siRNA techniques. The results demonstrated that the levels of NR5A2 mRNA and protein in the decidual tissues of women with PE were lower than those of normal pregnant women. However, the levels of NR5A1 mRNA and protein did not significantly differ between groups. The expression of NR5A2 was upregulated after in vitro decidualization, but the expression of NR5A1 remained low and showed no difference compared with that of the control cells. Knocking down of NR5A2 in human endometrial stromal cells (hESC) resulted in a significant reduction in their expression of decidualization markers (IGFBP1 and PRL) and signaling pathway molecules (WNT4 and BMP2) (P < 0.05). From these data, we concluded that NR5A2 is pivotal for the decidualization of decidual tissues and cultured human endometrial stromal cells. Disorders of the endometrium in decidual tissues may be associated with the abnormal decidualization thought to cause PE.

Show MeSH

Related in: MedlinePlus

Morphological changes of hESCs in the process of induced decidualization.(A), (B), and (C) show the morphology of control hESCs on days 0, 3, and 6. (D), (E), and (F) show the morphology of the induced hESCs on days 0, 3, and 6. Ctrl, control hESCs; db-cAMP+MPA, treated hESCs. Scale bars = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4696807&req=5

pone.0145968.g003: Morphological changes of hESCs in the process of induced decidualization.(A), (B), and (C) show the morphology of control hESCs on days 0, 3, and 6. (D), (E), and (F) show the morphology of the induced hESCs on days 0, 3, and 6. Ctrl, control hESCs; db-cAMP+MPA, treated hESCs. Scale bars = 50 μm.

Mentions: In vitro decidualization of hESCs was performed to investigate the involvement of NR5A1 and NR5A2 in this process. Firstly, the morphological changes during the decidualization of hESCs were evaluated in vitro. As shown in Fig 3A–3F, hESCs were elongated and had a fibroblast-like phenotype in the control sample (Fig 3E). The cells became rounded, relatively large epithelioid-like or polygonal decidual cells after treatment with db-cAMP and MPA for six days (Fig 3F).


Dysfunction of Liver Receptor Homolog-1 in Decidua: Possible Relevance to the Pathogenesis of Preeclampsia.

Zhang D, Cheng D, Liu T, Zhang Y, Chen ZJ, Zhang C - PLoS ONE (2015)

Morphological changes of hESCs in the process of induced decidualization.(A), (B), and (C) show the morphology of control hESCs on days 0, 3, and 6. (D), (E), and (F) show the morphology of the induced hESCs on days 0, 3, and 6. Ctrl, control hESCs; db-cAMP+MPA, treated hESCs. Scale bars = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696807&req=5

pone.0145968.g003: Morphological changes of hESCs in the process of induced decidualization.(A), (B), and (C) show the morphology of control hESCs on days 0, 3, and 6. (D), (E), and (F) show the morphology of the induced hESCs on days 0, 3, and 6. Ctrl, control hESCs; db-cAMP+MPA, treated hESCs. Scale bars = 50 μm.
Mentions: In vitro decidualization of hESCs was performed to investigate the involvement of NR5A1 and NR5A2 in this process. Firstly, the morphological changes during the decidualization of hESCs were evaluated in vitro. As shown in Fig 3A–3F, hESCs were elongated and had a fibroblast-like phenotype in the control sample (Fig 3E). The cells became rounded, relatively large epithelioid-like or polygonal decidual cells after treatment with db-cAMP and MPA for six days (Fig 3F).

Bottom Line: However, the results of this study indicated that insufficient decidualization plays a significant role.However, the levels of NR5A1 mRNA and protein did not significantly differ between groups.The expression of NR5A2 was upregulated after in vitro decidualization, but the expression of NR5A1 remained low and showed no difference compared with that of the control cells.

View Article: PubMed Central - PubMed

Affiliation: Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Preeclampsia (PE) is a multisystem disorder unique to Homo sapiens that is known to cause maternal and perinatal mortality and morbidity. Between 5-7% of all pregnancies are affected by PE and it is responsible for approximately 50,000 maternal deaths annually. The pathogenesis of PE remains poorly understood. However, the results of this study indicated that insufficient decidualization plays a significant role. NR5A1 and NR5A2 are orphan members of the Ftz-F1 subfamily of nuclear receptors and are involved in mammal follicular development, female reproduction, steroidogenesis, and decidualization. The expression of NR5A1 and NR5A2 in the human decidua and their functions during decidualization were investigated using in vitro cultured cells by real-time PCR, immunohistochemistry, western blotting, and siRNA techniques. The results demonstrated that the levels of NR5A2 mRNA and protein in the decidual tissues of women with PE were lower than those of normal pregnant women. However, the levels of NR5A1 mRNA and protein did not significantly differ between groups. The expression of NR5A2 was upregulated after in vitro decidualization, but the expression of NR5A1 remained low and showed no difference compared with that of the control cells. Knocking down of NR5A2 in human endometrial stromal cells (hESC) resulted in a significant reduction in their expression of decidualization markers (IGFBP1 and PRL) and signaling pathway molecules (WNT4 and BMP2) (P < 0.05). From these data, we concluded that NR5A2 is pivotal for the decidualization of decidual tissues and cultured human endometrial stromal cells. Disorders of the endometrium in decidual tissues may be associated with the abnormal decidualization thought to cause PE.

Show MeSH
Related in: MedlinePlus