Limits...
Dysfunction of Liver Receptor Homolog-1 in Decidua: Possible Relevance to the Pathogenesis of Preeclampsia.

Zhang D, Cheng D, Liu T, Zhang Y, Chen ZJ, Zhang C - PLoS ONE (2015)

Bottom Line: However, the results of this study indicated that insufficient decidualization plays a significant role.However, the levels of NR5A1 mRNA and protein did not significantly differ between groups.The expression of NR5A2 was upregulated after in vitro decidualization, but the expression of NR5A1 remained low and showed no difference compared with that of the control cells.

View Article: PubMed Central - PubMed

Affiliation: Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Preeclampsia (PE) is a multisystem disorder unique to Homo sapiens that is known to cause maternal and perinatal mortality and morbidity. Between 5-7% of all pregnancies are affected by PE and it is responsible for approximately 50,000 maternal deaths annually. The pathogenesis of PE remains poorly understood. However, the results of this study indicated that insufficient decidualization plays a significant role. NR5A1 and NR5A2 are orphan members of the Ftz-F1 subfamily of nuclear receptors and are involved in mammal follicular development, female reproduction, steroidogenesis, and decidualization. The expression of NR5A1 and NR5A2 in the human decidua and their functions during decidualization were investigated using in vitro cultured cells by real-time PCR, immunohistochemistry, western blotting, and siRNA techniques. The results demonstrated that the levels of NR5A2 mRNA and protein in the decidual tissues of women with PE were lower than those of normal pregnant women. However, the levels of NR5A1 mRNA and protein did not significantly differ between groups. The expression of NR5A2 was upregulated after in vitro decidualization, but the expression of NR5A1 remained low and showed no difference compared with that of the control cells. Knocking down of NR5A2 in human endometrial stromal cells (hESC) resulted in a significant reduction in their expression of decidualization markers (IGFBP1 and PRL) and signaling pathway molecules (WNT4 and BMP2) (P < 0.05). From these data, we concluded that NR5A2 is pivotal for the decidualization of decidual tissues and cultured human endometrial stromal cells. Disorders of the endometrium in decidual tissues may be associated with the abnormal decidualization thought to cause PE.

Show MeSH

Related in: MedlinePlus

NR5A1 and NR5A2 mRNA and protein expression in the decidual tissues of women with and without SPE.(A) The mRNA expression of NR5A1 and NR5A2 in the decidual tissues of subjects from the NP and SPE groups. (B) The protein expression of NR5A1 and NR5A2 in the decidual tissues of subjects from the NP and SPE groups. (C) The relative expression levels of the NR5A1 and NR5A2 proteins compared with that of β-actin (n = 23 for each group). NP, normal pregnancy group. SPE, severe preeclampsia group. The data were shown as mean ± SEM, *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4696807&req=5

pone.0145968.g001: NR5A1 and NR5A2 mRNA and protein expression in the decidual tissues of women with and without SPE.(A) The mRNA expression of NR5A1 and NR5A2 in the decidual tissues of subjects from the NP and SPE groups. (B) The protein expression of NR5A1 and NR5A2 in the decidual tissues of subjects from the NP and SPE groups. (C) The relative expression levels of the NR5A1 and NR5A2 proteins compared with that of β-actin (n = 23 for each group). NP, normal pregnancy group. SPE, severe preeclampsia group. The data were shown as mean ± SEM, *P < 0.05.

Mentions: Decidual tissues from 46 women were examined for the expression of NR5A1 and NR5A2. Of these tissue samples, 23 were obtained from NP women and the rest from women with SPE. The transcribed and translated levels of NR5A2 were significantly lower in the SPE group than those in the NP group (Fig 1A, 1B and 1C). As for NR5A1, there were no differences in the transcribed or translated levels between the two groups (Fig 1A, 1B and 1C). Immunohistochemistry was performed to locate the NR5A1 and NR5A2 proteins (Fig 2). The NR5A1 and NR5A2 proteins were both detected in decidual cells and NR5A2 protein was less abundant in the decidual cells of the SPE group relative to that of the NP group (Fig 2C and 2D). However, there was no difference in NR5A1 protein abundance between the SPE and NP groups (Fig 2A and 2B).


Dysfunction of Liver Receptor Homolog-1 in Decidua: Possible Relevance to the Pathogenesis of Preeclampsia.

Zhang D, Cheng D, Liu T, Zhang Y, Chen ZJ, Zhang C - PLoS ONE (2015)

NR5A1 and NR5A2 mRNA and protein expression in the decidual tissues of women with and without SPE.(A) The mRNA expression of NR5A1 and NR5A2 in the decidual tissues of subjects from the NP and SPE groups. (B) The protein expression of NR5A1 and NR5A2 in the decidual tissues of subjects from the NP and SPE groups. (C) The relative expression levels of the NR5A1 and NR5A2 proteins compared with that of β-actin (n = 23 for each group). NP, normal pregnancy group. SPE, severe preeclampsia group. The data were shown as mean ± SEM, *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696807&req=5

pone.0145968.g001: NR5A1 and NR5A2 mRNA and protein expression in the decidual tissues of women with and without SPE.(A) The mRNA expression of NR5A1 and NR5A2 in the decidual tissues of subjects from the NP and SPE groups. (B) The protein expression of NR5A1 and NR5A2 in the decidual tissues of subjects from the NP and SPE groups. (C) The relative expression levels of the NR5A1 and NR5A2 proteins compared with that of β-actin (n = 23 for each group). NP, normal pregnancy group. SPE, severe preeclampsia group. The data were shown as mean ± SEM, *P < 0.05.
Mentions: Decidual tissues from 46 women were examined for the expression of NR5A1 and NR5A2. Of these tissue samples, 23 were obtained from NP women and the rest from women with SPE. The transcribed and translated levels of NR5A2 were significantly lower in the SPE group than those in the NP group (Fig 1A, 1B and 1C). As for NR5A1, there were no differences in the transcribed or translated levels between the two groups (Fig 1A, 1B and 1C). Immunohistochemistry was performed to locate the NR5A1 and NR5A2 proteins (Fig 2). The NR5A1 and NR5A2 proteins were both detected in decidual cells and NR5A2 protein was less abundant in the decidual cells of the SPE group relative to that of the NP group (Fig 2C and 2D). However, there was no difference in NR5A1 protein abundance between the SPE and NP groups (Fig 2A and 2B).

Bottom Line: However, the results of this study indicated that insufficient decidualization plays a significant role.However, the levels of NR5A1 mRNA and protein did not significantly differ between groups.The expression of NR5A2 was upregulated after in vitro decidualization, but the expression of NR5A1 remained low and showed no difference compared with that of the control cells.

View Article: PubMed Central - PubMed

Affiliation: Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Preeclampsia (PE) is a multisystem disorder unique to Homo sapiens that is known to cause maternal and perinatal mortality and morbidity. Between 5-7% of all pregnancies are affected by PE and it is responsible for approximately 50,000 maternal deaths annually. The pathogenesis of PE remains poorly understood. However, the results of this study indicated that insufficient decidualization plays a significant role. NR5A1 and NR5A2 are orphan members of the Ftz-F1 subfamily of nuclear receptors and are involved in mammal follicular development, female reproduction, steroidogenesis, and decidualization. The expression of NR5A1 and NR5A2 in the human decidua and their functions during decidualization were investigated using in vitro cultured cells by real-time PCR, immunohistochemistry, western blotting, and siRNA techniques. The results demonstrated that the levels of NR5A2 mRNA and protein in the decidual tissues of women with PE were lower than those of normal pregnant women. However, the levels of NR5A1 mRNA and protein did not significantly differ between groups. The expression of NR5A2 was upregulated after in vitro decidualization, but the expression of NR5A1 remained low and showed no difference compared with that of the control cells. Knocking down of NR5A2 in human endometrial stromal cells (hESC) resulted in a significant reduction in their expression of decidualization markers (IGFBP1 and PRL) and signaling pathway molecules (WNT4 and BMP2) (P < 0.05). From these data, we concluded that NR5A2 is pivotal for the decidualization of decidual tissues and cultured human endometrial stromal cells. Disorders of the endometrium in decidual tissues may be associated with the abnormal decidualization thought to cause PE.

Show MeSH
Related in: MedlinePlus