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A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.

Sun X, Sun M, Jia B, Chen C, Qin Z, Yang K, Shen Y, Meiping Z, Mingyang C, Zhu Y - PLoS ONE (2015)

Bottom Line: Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity.As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes.Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.

ABSTRACT
It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

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GsGF14o overexpression up-regulated the transcript levels of ABA induced genes.Transcription levels of ABA induced genes were determined by quantitative real-time PCR, and ACTIN2 was used as an internal control. Results were normalized to the corresponding transcript levels of WT plants at 0 h. *, P < 0.05; **, P < 0.01 by Student’s t test.
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pone.0146163.g010: GsGF14o overexpression up-regulated the transcript levels of ABA induced genes.Transcription levels of ABA induced genes were determined by quantitative real-time PCR, and ACTIN2 was used as an internal control. Results were normalized to the corresponding transcript levels of WT plants at 0 h. *, P < 0.05; **, P < 0.01 by Student’s t test.

Mentions: ABA stress is suggested to induce the expression of a number of genes, which are involved in plant responses to environmental stress [52]. In this study, we checked the expression of several ABA induced genes, including RD29A, RD29B, RD22, COR15A, KIN1, and RAB18, all of which have ABRE cis-elements in the promoter region. Quantitative real-time PCR results showed that expression levels of all these genes were greatly and rapidly induced by ABA stress in both WT and OX plants. However, their expression levels in OX plants were significantly higher than that in WT (Fig 10). In conclusion, these results suggested that GsGF14o overexpression in Arabidopsis up-regulated the expression levels of ABA induced genes.


A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.

Sun X, Sun M, Jia B, Chen C, Qin Z, Yang K, Shen Y, Meiping Z, Mingyang C, Zhu Y - PLoS ONE (2015)

GsGF14o overexpression up-regulated the transcript levels of ABA induced genes.Transcription levels of ABA induced genes were determined by quantitative real-time PCR, and ACTIN2 was used as an internal control. Results were normalized to the corresponding transcript levels of WT plants at 0 h. *, P < 0.05; **, P < 0.01 by Student’s t test.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4696740&req=5

pone.0146163.g010: GsGF14o overexpression up-regulated the transcript levels of ABA induced genes.Transcription levels of ABA induced genes were determined by quantitative real-time PCR, and ACTIN2 was used as an internal control. Results were normalized to the corresponding transcript levels of WT plants at 0 h. *, P < 0.05; **, P < 0.01 by Student’s t test.
Mentions: ABA stress is suggested to induce the expression of a number of genes, which are involved in plant responses to environmental stress [52]. In this study, we checked the expression of several ABA induced genes, including RD29A, RD29B, RD22, COR15A, KIN1, and RAB18, all of which have ABRE cis-elements in the promoter region. Quantitative real-time PCR results showed that expression levels of all these genes were greatly and rapidly induced by ABA stress in both WT and OX plants. However, their expression levels in OX plants were significantly higher than that in WT (Fig 10). In conclusion, these results suggested that GsGF14o overexpression in Arabidopsis up-regulated the expression levels of ABA induced genes.

Bottom Line: Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity.As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes.Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.

ABSTRACT
It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

Show MeSH
Related in: MedlinePlus