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A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.

Sun X, Sun M, Jia B, Chen C, Qin Z, Yang K, Shen Y, Meiping Z, Mingyang C, Zhu Y - PLoS ONE (2015)

Bottom Line: Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity.As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes.Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.

ABSTRACT
It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

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Structural analysis of the Glycine soja 14-3-3 protein GsGF14o.(A) Structural comparison of GsGF14o and Nt14-3-3 (PDB: 2098B). GsGF14o structure was shown in cartoon, while Nt14-3-3 structure was shown by using backbone trace. (B) Monomeric structure of the GsGF14o protein. (C) Predicted binding model of GsGF14o. Each structure is rotated 90° to show different view sides of the protein.
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pone.0146163.g006: Structural analysis of the Glycine soja 14-3-3 protein GsGF14o.(A) Structural comparison of GsGF14o and Nt14-3-3 (PDB: 2098B). GsGF14o structure was shown in cartoon, while Nt14-3-3 structure was shown by using backbone trace. (B) Monomeric structure of the GsGF14o protein. (C) Predicted binding model of GsGF14o. Each structure is rotated 90° to show different view sides of the protein.

Mentions: To get better understanding of the conformational features of GsGF14o protein, we further predicted the three-dimensional structure of GsGF14o by using the I-TASSER one-line software. As shown in Fig 6A, GsGF14o showed the highest similarity in terms of conformational structure to Nt14-3-3 (PDB: 2o98B) in the Protein Data Bank database (http://www.rcsb.org/pdb/home/home.do). Results of the three-dimensional structure analysis also confirmed that GsGF14o consisted of a bundle of nine α-helices (α1 to α9), which were organized into groups of two (α1 and α2), two (α3 and α4), two (α5 and α6), and three (α7, α8 and α9) helices (Fig 6B). Out of them, the first four helices were reported to be essential for dimer formation [47], and helices α3, α5, α7 and α9 could form a conserved peptide-binding groove (Fig 6C).


A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.

Sun X, Sun M, Jia B, Chen C, Qin Z, Yang K, Shen Y, Meiping Z, Mingyang C, Zhu Y - PLoS ONE (2015)

Structural analysis of the Glycine soja 14-3-3 protein GsGF14o.(A) Structural comparison of GsGF14o and Nt14-3-3 (PDB: 2098B). GsGF14o structure was shown in cartoon, while Nt14-3-3 structure was shown by using backbone trace. (B) Monomeric structure of the GsGF14o protein. (C) Predicted binding model of GsGF14o. Each structure is rotated 90° to show different view sides of the protein.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696740&req=5

pone.0146163.g006: Structural analysis of the Glycine soja 14-3-3 protein GsGF14o.(A) Structural comparison of GsGF14o and Nt14-3-3 (PDB: 2098B). GsGF14o structure was shown in cartoon, while Nt14-3-3 structure was shown by using backbone trace. (B) Monomeric structure of the GsGF14o protein. (C) Predicted binding model of GsGF14o. Each structure is rotated 90° to show different view sides of the protein.
Mentions: To get better understanding of the conformational features of GsGF14o protein, we further predicted the three-dimensional structure of GsGF14o by using the I-TASSER one-line software. As shown in Fig 6A, GsGF14o showed the highest similarity in terms of conformational structure to Nt14-3-3 (PDB: 2o98B) in the Protein Data Bank database (http://www.rcsb.org/pdb/home/home.do). Results of the three-dimensional structure analysis also confirmed that GsGF14o consisted of a bundle of nine α-helices (α1 to α9), which were organized into groups of two (α1 and α2), two (α3 and α4), two (α5 and α6), and three (α7, α8 and α9) helices (Fig 6B). Out of them, the first four helices were reported to be essential for dimer formation [47], and helices α3, α5, α7 and α9 could form a conserved peptide-binding groove (Fig 6C).

Bottom Line: Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity.As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes.Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.

ABSTRACT
It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

Show MeSH
Related in: MedlinePlus