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A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.

Sun X, Sun M, Jia B, Chen C, Qin Z, Yang K, Shen Y, Meiping Z, Mingyang C, Zhu Y - PLoS ONE (2015)

Bottom Line: Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity.As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes.Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.

ABSTRACT
It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

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Segmental duplication analyses of soybean 14-3-3 family genes in Group I (A), Group II (B), Group III (C), and Group IV (D).The segmental duplication of soybean 14-3-3 genes was obtained based on the locus search at PGDD website.
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pone.0146163.g005: Segmental duplication analyses of soybean 14-3-3 family genes in Group I (A), Group II (B), Group III (C), and Group IV (D).The segmental duplication of soybean 14-3-3 genes was obtained based on the locus search at PGDD website.

Mentions: It is reported that soybean underwent at least two rounds of genome wide duplications approximately 13 and 59 million years ago [45]. Based on the locus search at PGDD website (Plant Genome Duplication Database, http://chibba.agtec.uga.edu/duplication/)[46], we found that all of the nine 14-3-3 paralogous gene pairs in soybean genome were generated by segmental duplication (Fig 5). Remarkably, four genes in group IV (SGF14o, SGF14p, SGF14n, and SGF14d) were supposed to be within the same duplication blocks (Fig 5D). Moreover, the four 14-3-3 proteins displayed over 80% sequence identity (S2 Fig) and showed closely related location in the phylogenetic tree (Fig 2 and Fig 3A). These findings suggested that these two paralogous gene pairs might originate from a common ancestor, which firstly underwent tandem duplication prior to segmental duplication.


A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.

Sun X, Sun M, Jia B, Chen C, Qin Z, Yang K, Shen Y, Meiping Z, Mingyang C, Zhu Y - PLoS ONE (2015)

Segmental duplication analyses of soybean 14-3-3 family genes in Group I (A), Group II (B), Group III (C), and Group IV (D).The segmental duplication of soybean 14-3-3 genes was obtained based on the locus search at PGDD website.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696740&req=5

pone.0146163.g005: Segmental duplication analyses of soybean 14-3-3 family genes in Group I (A), Group II (B), Group III (C), and Group IV (D).The segmental duplication of soybean 14-3-3 genes was obtained based on the locus search at PGDD website.
Mentions: It is reported that soybean underwent at least two rounds of genome wide duplications approximately 13 and 59 million years ago [45]. Based on the locus search at PGDD website (Plant Genome Duplication Database, http://chibba.agtec.uga.edu/duplication/)[46], we found that all of the nine 14-3-3 paralogous gene pairs in soybean genome were generated by segmental duplication (Fig 5). Remarkably, four genes in group IV (SGF14o, SGF14p, SGF14n, and SGF14d) were supposed to be within the same duplication blocks (Fig 5D). Moreover, the four 14-3-3 proteins displayed over 80% sequence identity (S2 Fig) and showed closely related location in the phylogenetic tree (Fig 2 and Fig 3A). These findings suggested that these two paralogous gene pairs might originate from a common ancestor, which firstly underwent tandem duplication prior to segmental duplication.

Bottom Line: Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity.As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes.Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.

ABSTRACT
It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

Show MeSH
Related in: MedlinePlus