Limits...
A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.

Sun X, Sun M, Jia B, Chen C, Qin Z, Yang K, Shen Y, Meiping Z, Mingyang C, Zhu Y - PLoS ONE (2015)

Bottom Line: Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity.As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes.Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.

ABSTRACT
It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

Show MeSH

Related in: MedlinePlus

Phylogenetic analysis and exon-intron structures of soybean 14-3-3 family members.(A) The phylogenetic tree of soybean 14-3-3 family proteins. (B) The exon-intron structures of soybean 14-3-3 family genes. Yellow and blue boxes represented exons and lines represented introns.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4696740&req=5

pone.0146163.g003: Phylogenetic analysis and exon-intron structures of soybean 14-3-3 family members.(A) The phylogenetic tree of soybean 14-3-3 family proteins. (B) The exon-intron structures of soybean 14-3-3 family genes. Yellow and blue boxes represented exons and lines represented introns.

Mentions: Furthermore, the phylogenetic comparison of the soybean, rice and Arabidopsis 14-3-3s revealed that these 14-3-3 proteins could be clustered into two subfamilies, the ε subfamily and the non-ε subfamily (Fig 2), the same as described previously [38]. Subsequently, the non-ε subfamily was further divided into two groups (Group I-II) and the ε subfamily was divided into three groups (Group III-V). Notably, group I, III and IV only contained soybean and Arabidopsis 14-3-3 proteins, while group V only included rice and Arabidopsis 14-3-3s. As expected, soybean 14-3-3 family was only consisted of four groups (Group I-IV, Fig 3A), without the fifth group in Fig 2.


A 14-3-3 Family Protein from Wild Soybean (Glycine Soja) Regulates ABA Sensitivity in Arabidopsis.

Sun X, Sun M, Jia B, Chen C, Qin Z, Yang K, Shen Y, Meiping Z, Mingyang C, Zhu Y - PLoS ONE (2015)

Phylogenetic analysis and exon-intron structures of soybean 14-3-3 family members.(A) The phylogenetic tree of soybean 14-3-3 family proteins. (B) The exon-intron structures of soybean 14-3-3 family genes. Yellow and blue boxes represented exons and lines represented introns.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696740&req=5

pone.0146163.g003: Phylogenetic analysis and exon-intron structures of soybean 14-3-3 family members.(A) The phylogenetic tree of soybean 14-3-3 family proteins. (B) The exon-intron structures of soybean 14-3-3 family genes. Yellow and blue boxes represented exons and lines represented introns.
Mentions: Furthermore, the phylogenetic comparison of the soybean, rice and Arabidopsis 14-3-3s revealed that these 14-3-3 proteins could be clustered into two subfamilies, the ε subfamily and the non-ε subfamily (Fig 2), the same as described previously [38]. Subsequently, the non-ε subfamily was further divided into two groups (Group I-II) and the ε subfamily was divided into three groups (Group III-V). Notably, group I, III and IV only contained soybean and Arabidopsis 14-3-3 proteins, while group V only included rice and Arabidopsis 14-3-3s. As expected, soybean 14-3-3 family was only consisted of four groups (Group I-IV, Fig 3A), without the fifth group in Fig 2.

Bottom Line: Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity.As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes.Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.

ABSTRACT
It is widely accepted that the 14-3-3 family proteins are key regulators of multiple stress signal transduction cascades. By conducting genome-wide analysis, researchers have identified the soybean 14-3-3 family proteins; however, until now, there is still no direct genetic evidence showing the involvement of soybean 14-3-3s in ABA responses. Hence, in this study, based on the latest Glycine max genome on Phytozome v10.3, we initially analyzed the evolutionary relationship, genome organization, gene structure and duplication, and three-dimensional structure of soybean 14-3-3 family proteins systematically. Our results suggested that soybean 14-3-3 family was highly evolutionary conserved and possessed segmental duplication in evolution. Then, based on our previous functional characterization of a Glycine soja 14-3-3 protein GsGF14o in drought stress responses, we further investigated the expression characteristics of GsGF14o in detail, and demonstrated its positive roles in ABA sensitivity. Quantitative real-time PCR analyses in Glycine soja seedlings and GUS activity assays in PGsGF14O:GUS transgenic Arabidopsis showed that GsGF14o expression was moderately and rapidly induced by ABA treatment. As expected, GsGF14o overexpression in Arabidopsis augmented the ABA inhibition of seed germination and seedling growth, promoted the ABA induced stomata closure, and up-regulated the expression levels of ABA induced genes. Moreover, through yeast two hybrid analyses, we further demonstrated that GsGF14o physically interacted with the AREB/ABF transcription factors in yeast cells. Taken together, results presented in this study strongly suggested that GsGF14o played an important role in regulation of ABA sensitivity in Arabidopsis.

Show MeSH
Related in: MedlinePlus