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Porcine Epidemic Diarrhea Virus and Discovery of a Recombinant Swine Enteric Coronavirus, Italy.

Boniotti MB, Papetti A, Lavazza A, Alborali G, Sozzi E, Chiapponi C, Faccini S, Bonilauri P, Cordioli P, Marthaler D - Emerging Infect. Dis. (2016)

Bottom Line: Porcine epidemic diarrhea virus (PEDV) has been detected sporadically in Italy since the 1990s.We report the phylogenetic relationship of swine enteric coronaviruses collected in Italy during 2007-2014 and identify a drastic shift in PEDV strain variability and a new swine enteric coronavirus generated by recombination of transmissible gastroenteritis virus and PEDV.

View Article: PubMed Central - PubMed

ABSTRACT
Porcine epidemic diarrhea virus (PEDV) has been detected sporadically in Italy since the 1990s. We report the phylogenetic relationship of swine enteric coronaviruses collected in Italy during 2007-2014 and identify a drastic shift in PEDV strain variability and a new swine enteric coronavirus generated by recombination of transmissible gastroenteritis virus and PEDV.

No MeSH data available.


Related in: MedlinePlus

Potential recombination points in the SeCoV strains in study of swine enteric coronaviruses in Italy. The potential parent strains H16 (TGEV) and CV777 (PEDV) are shown in teal and purple, respectively. Arrows indicate recombinant breakpoints. PEDV, porcine epidemic diarrhea virus; TGEV, transmissible gastroenteritis virus; seCoV, swine enteric coronavirus.
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Figure 2: Potential recombination points in the SeCoV strains in study of swine enteric coronaviruses in Italy. The potential parent strains H16 (TGEV) and CV777 (PEDV) are shown in teal and purple, respectively. Arrows indicate recombinant breakpoints. PEDV, porcine epidemic diarrhea virus; TGEV, transmissible gastroenteritis virus; seCoV, swine enteric coronavirus.

Mentions: One strain from each cluster was selected for whole genome sequencing (Technical Appendix). Unfortunately, the whole genome was obtained from only clusters I and II (PEDV/Italy/7239/2009 and SeCoV/Italy/213306/2009, respectively; Figure 1, panel B). Recombination analysis was conducted on the 2 whole genomes and was not detected in PEDV/Italy/7239/2009. Recombination was detected in SeCoV/Italy/213306/2009 at position 20636 and 24867 of PEDV CV777 and at position 20366 and 24996 of TGEV H16 (Figure 2), suggesting the occurrence of a recombination event between a PEDV and a TGEV. The complete S gene of SeCoV/Italy/213306/2009 shared 92% and 90% nt identity with the prototype European strain PEDV CV777 and the original highly virulent North American strain Colorado 2013, respectively, and the remaining genome shared a 97% nt identity with the virulent strains TGEV H16 and TGEV Miller M6. Whole-genome analysis of PEDV/Italy/7239/2009 showed that it grouped with the global PEDV strains (6) and shared ≈97% nt identity with PEDV strains CV777, DR13 virulent, and North American S-INDEL strain OH851 (Table 2).


Porcine Epidemic Diarrhea Virus and Discovery of a Recombinant Swine Enteric Coronavirus, Italy.

Boniotti MB, Papetti A, Lavazza A, Alborali G, Sozzi E, Chiapponi C, Faccini S, Bonilauri P, Cordioli P, Marthaler D - Emerging Infect. Dis. (2016)

Potential recombination points in the SeCoV strains in study of swine enteric coronaviruses in Italy. The potential parent strains H16 (TGEV) and CV777 (PEDV) are shown in teal and purple, respectively. Arrows indicate recombinant breakpoints. PEDV, porcine epidemic diarrhea virus; TGEV, transmissible gastroenteritis virus; seCoV, swine enteric coronavirus.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4696687&req=5

Figure 2: Potential recombination points in the SeCoV strains in study of swine enteric coronaviruses in Italy. The potential parent strains H16 (TGEV) and CV777 (PEDV) are shown in teal and purple, respectively. Arrows indicate recombinant breakpoints. PEDV, porcine epidemic diarrhea virus; TGEV, transmissible gastroenteritis virus; seCoV, swine enteric coronavirus.
Mentions: One strain from each cluster was selected for whole genome sequencing (Technical Appendix). Unfortunately, the whole genome was obtained from only clusters I and II (PEDV/Italy/7239/2009 and SeCoV/Italy/213306/2009, respectively; Figure 1, panel B). Recombination analysis was conducted on the 2 whole genomes and was not detected in PEDV/Italy/7239/2009. Recombination was detected in SeCoV/Italy/213306/2009 at position 20636 and 24867 of PEDV CV777 and at position 20366 and 24996 of TGEV H16 (Figure 2), suggesting the occurrence of a recombination event between a PEDV and a TGEV. The complete S gene of SeCoV/Italy/213306/2009 shared 92% and 90% nt identity with the prototype European strain PEDV CV777 and the original highly virulent North American strain Colorado 2013, respectively, and the remaining genome shared a 97% nt identity with the virulent strains TGEV H16 and TGEV Miller M6. Whole-genome analysis of PEDV/Italy/7239/2009 showed that it grouped with the global PEDV strains (6) and shared ≈97% nt identity with PEDV strains CV777, DR13 virulent, and North American S-INDEL strain OH851 (Table 2).

Bottom Line: Porcine epidemic diarrhea virus (PEDV) has been detected sporadically in Italy since the 1990s.We report the phylogenetic relationship of swine enteric coronaviruses collected in Italy during 2007-2014 and identify a drastic shift in PEDV strain variability and a new swine enteric coronavirus generated by recombination of transmissible gastroenteritis virus and PEDV.

View Article: PubMed Central - PubMed

ABSTRACT
Porcine epidemic diarrhea virus (PEDV) has been detected sporadically in Italy since the 1990s. We report the phylogenetic relationship of swine enteric coronaviruses collected in Italy during 2007-2014 and identify a drastic shift in PEDV strain variability and a new swine enteric coronavirus generated by recombination of transmissible gastroenteritis virus and PEDV.

No MeSH data available.


Related in: MedlinePlus