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Identification of a New Marine Bacterial Strain SD8 and Optimization of Its Culture Conditions for Producing Alkaline Protease.

Cui H, Yang M, Wang L, Xian CJ - PLoS ONE (2015)

Bottom Line: While much attention has been given to marine microorganisms for production of enzymes, which in general are relatively more stable and active compared to those from plants and animals, studies on alkaline protease production from marine microorganisms have been very limited.Strain SD8 was initially classified to belong to genus Pseudomonas by morphological, physiological and biochemical characterizations, and then through 16S rDNA sequence it was identified to be likely Pseudomonas hibiscicola.This was the first report of strain Pseudomonas hibiscicola secreting alkaline protease, and the data for its optimal cultural conditions for alkaline protease production has laid a foundation for future exploration for the potential use of SD8 strain for alkaline protease production.

View Article: PubMed Central - PubMed

Affiliation: College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, China.

ABSTRACT
While much attention has been given to marine microorganisms for production of enzymes, which in general are relatively more stable and active compared to those from plants and animals, studies on alkaline protease production from marine microorganisms have been very limited. In the present study, the alkaline protease producing marine bacterial strain SD8 isolated from sea muds in the Geziwo Qinhuangdao sea area of China was characterized and its optimal culture conditions were investigated. Strain SD8 was initially classified to belong to genus Pseudomonas by morphological, physiological and biochemical characterizations, and then through 16S rDNA sequence it was identified to be likely Pseudomonas hibiscicola. In addition, the culture mediums, carbon sources and culture conditions of strain SD8 were optimized for maximum production of alkaline protease. Optimum enzyme production (236U/mL when cultured bacteria being at 0.75 mg dry weight/mL fermentation broth) was obtained when the isolate at a 3% inoculum size was grown in LB medium at 20 mL medium/100mL Erlenmeyer flask for 48h culture at 30°C with an initial of pH 7.5. This was the first report of strain Pseudomonas hibiscicola secreting alkaline protease, and the data for its optimal cultural conditions for alkaline protease production has laid a foundation for future exploration for the potential use of SD8 strain for alkaline protease production.

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Effects of initial pH in influencing the production of alkaline protease from strain SD8.The single factor investigated was selected at optimal conditions. *P<0.05 and **P<0.01 compared to pH 7.5. All the data were given as means ± SEM (n = 3).
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pone.0146067.g007: Effects of initial pH in influencing the production of alkaline protease from strain SD8.The single factor investigated was selected at optimal conditions. *P<0.05 and **P<0.01 compared to pH 7.5. All the data were given as means ± SEM (n = 3).

Mentions: The current study also examined influences of culture medium pH and temperatures and found a gradual increase in the protease production in strain SD8 with increasing pH, with the optimum being at pH 7.5 (185U/mL) (Fig 7). At pH 8.5, the protease activity was 179 U/mL, which was lower than that of pH 7.5 (P<0.05). In either lower pH (5.5 or 6.5) or higher pH (9.5 or 10.5) cultures, the protease activity was significantly lower than that of pH 7.5 (P<0.01). When the incubation temperatures were 37 and 30°C, the alkaline protease activities of the cultures were 185 and 216 U/mL, respectively. Thus, 30°C was chosen for carrying out the subsequent work.


Identification of a New Marine Bacterial Strain SD8 and Optimization of Its Culture Conditions for Producing Alkaline Protease.

Cui H, Yang M, Wang L, Xian CJ - PLoS ONE (2015)

Effects of initial pH in influencing the production of alkaline protease from strain SD8.The single factor investigated was selected at optimal conditions. *P<0.05 and **P<0.01 compared to pH 7.5. All the data were given as means ± SEM (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696672&req=5

pone.0146067.g007: Effects of initial pH in influencing the production of alkaline protease from strain SD8.The single factor investigated was selected at optimal conditions. *P<0.05 and **P<0.01 compared to pH 7.5. All the data were given as means ± SEM (n = 3).
Mentions: The current study also examined influences of culture medium pH and temperatures and found a gradual increase in the protease production in strain SD8 with increasing pH, with the optimum being at pH 7.5 (185U/mL) (Fig 7). At pH 8.5, the protease activity was 179 U/mL, which was lower than that of pH 7.5 (P<0.05). In either lower pH (5.5 or 6.5) or higher pH (9.5 or 10.5) cultures, the protease activity was significantly lower than that of pH 7.5 (P<0.01). When the incubation temperatures were 37 and 30°C, the alkaline protease activities of the cultures were 185 and 216 U/mL, respectively. Thus, 30°C was chosen for carrying out the subsequent work.

Bottom Line: While much attention has been given to marine microorganisms for production of enzymes, which in general are relatively more stable and active compared to those from plants and animals, studies on alkaline protease production from marine microorganisms have been very limited.Strain SD8 was initially classified to belong to genus Pseudomonas by morphological, physiological and biochemical characterizations, and then through 16S rDNA sequence it was identified to be likely Pseudomonas hibiscicola.This was the first report of strain Pseudomonas hibiscicola secreting alkaline protease, and the data for its optimal cultural conditions for alkaline protease production has laid a foundation for future exploration for the potential use of SD8 strain for alkaline protease production.

View Article: PubMed Central - PubMed

Affiliation: College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, China.

ABSTRACT
While much attention has been given to marine microorganisms for production of enzymes, which in general are relatively more stable and active compared to those from plants and animals, studies on alkaline protease production from marine microorganisms have been very limited. In the present study, the alkaline protease producing marine bacterial strain SD8 isolated from sea muds in the Geziwo Qinhuangdao sea area of China was characterized and its optimal culture conditions were investigated. Strain SD8 was initially classified to belong to genus Pseudomonas by morphological, physiological and biochemical characterizations, and then through 16S rDNA sequence it was identified to be likely Pseudomonas hibiscicola. In addition, the culture mediums, carbon sources and culture conditions of strain SD8 were optimized for maximum production of alkaline protease. Optimum enzyme production (236U/mL when cultured bacteria being at 0.75 mg dry weight/mL fermentation broth) was obtained when the isolate at a 3% inoculum size was grown in LB medium at 20 mL medium/100mL Erlenmeyer flask for 48h culture at 30°C with an initial of pH 7.5. This was the first report of strain Pseudomonas hibiscicola secreting alkaline protease, and the data for its optimal cultural conditions for alkaline protease production has laid a foundation for future exploration for the potential use of SD8 strain for alkaline protease production.

Show MeSH
Related in: MedlinePlus