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Penicillium excelsum sp. nov from the Brazil Nut Tree Ecosystem in the Amazon Basin'.

Taniwaki MH, Pitt JI, Iamanaka BT, Massi FP, Fungaro MH, Frisvad JC - PLoS ONE (2015)

Bottom Line: It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest.The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences.The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciência e Qualidade de Alimentos, Instituto de Tecnologia de Alimentos, Campinas, São Paulo, Brazil.

ABSTRACT
A new Penicillium species, P. excelsum, is described here using morphological characters, extrolite and partial sequence data from the ITS, β-tubulin and calmodulin genes. It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest. The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences. The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype.

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Related in: MedlinePlus

Plate of Czapek yeast extract agar (CYA) with Penicillium excelsum, opaqueness of petri dish lid after 7 days of incubation at 25°C.
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pone.0143189.g004: Plate of Czapek yeast extract agar (CYA) with Penicillium excelsum, opaqueness of petri dish lid after 7 days of incubation at 25°C.

Mentions: This species is also distinguished by a unique profile of extrolytes and by unique DNA sequences in the ITS, BenA and CaM genes. This species is also notable in that cultures on CYA, MEA and YES agar cause the polystyrene plastic in Petri dishes to become opaque over time (Fig 4). The opaqueness cannot be removed using a scapel, as the chemical reaction with the plastic lids was irreversible. A volatile compound produced by the fungus as it grows must be responsible. A preliminary examination of the volatiles from P. excelsum showed that it produced large amounts of acetic acid. An HPLD-DAD analysis of the opaque layer on the Petri dish lid revealed no detectable extrolites, indicating that the compound responsible for the opaqueness is without a chromophore. This effect has not been reported from any Penicillium or Aspergillus species. Further studies will be carried out in order to determine these compounds.


Penicillium excelsum sp. nov from the Brazil Nut Tree Ecosystem in the Amazon Basin'.

Taniwaki MH, Pitt JI, Iamanaka BT, Massi FP, Fungaro MH, Frisvad JC - PLoS ONE (2015)

Plate of Czapek yeast extract agar (CYA) with Penicillium excelsum, opaqueness of petri dish lid after 7 days of incubation at 25°C.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696661&req=5

pone.0143189.g004: Plate of Czapek yeast extract agar (CYA) with Penicillium excelsum, opaqueness of petri dish lid after 7 days of incubation at 25°C.
Mentions: This species is also distinguished by a unique profile of extrolytes and by unique DNA sequences in the ITS, BenA and CaM genes. This species is also notable in that cultures on CYA, MEA and YES agar cause the polystyrene plastic in Petri dishes to become opaque over time (Fig 4). The opaqueness cannot be removed using a scapel, as the chemical reaction with the plastic lids was irreversible. A volatile compound produced by the fungus as it grows must be responsible. A preliminary examination of the volatiles from P. excelsum showed that it produced large amounts of acetic acid. An HPLD-DAD analysis of the opaque layer on the Petri dish lid revealed no detectable extrolites, indicating that the compound responsible for the opaqueness is without a chromophore. This effect has not been reported from any Penicillium or Aspergillus species. Further studies will be carried out in order to determine these compounds.

Bottom Line: It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest.The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences.The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciência e Qualidade de Alimentos, Instituto de Tecnologia de Alimentos, Campinas, São Paulo, Brazil.

ABSTRACT
A new Penicillium species, P. excelsum, is described here using morphological characters, extrolite and partial sequence data from the ITS, β-tubulin and calmodulin genes. It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest. The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences. The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype.

Show MeSH
Related in: MedlinePlus