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Penicillium excelsum sp. nov from the Brazil Nut Tree Ecosystem in the Amazon Basin'.

Taniwaki MH, Pitt JI, Iamanaka BT, Massi FP, Fungaro MH, Frisvad JC - PLoS ONE (2015)

Bottom Line: It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest.The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences.The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciência e Qualidade de Alimentos, Instituto de Tecnologia de Alimentos, Campinas, São Paulo, Brazil.

ABSTRACT
A new Penicillium species, P. excelsum, is described here using morphological characters, extrolite and partial sequence data from the ITS, β-tubulin and calmodulin genes. It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest. The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences. The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype.

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Maximum-likelihood tree reconstructed from the partial β-tubulin (BenA) gene sequences aligned with corresponding sequences of Penicillium section Lanata-Divaricata deposited in public databases.Numbers at branch nodes refer to bootstrap values (1,000 replicates), only values of >70% are shown.
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pone.0143189.g002: Maximum-likelihood tree reconstructed from the partial β-tubulin (BenA) gene sequences aligned with corresponding sequences of Penicillium section Lanata-Divaricata deposited in public databases.Numbers at branch nodes refer to bootstrap values (1,000 replicates), only values of >70% are shown.

Mentions: HPLC-DAD analysis of extracts showed that several strains of P. excelsum produce andrastin A, penicillic acid, while some also produce xanthoepocin. Strain ITAL 3000 also produced curvulic acid. Related species also produce penicillic acid, for example P. brasilianum, P. cremeogriseum, P. ochrochloron P. pulvillorum and P. vanderhammenii [24, 26, 27]. P. pulvillorum and P. simplicissimum have also been reported to produce andrastin A, and P. brasilianum, P. ochrochloron, P. pulvillorum, P. rolfsii, P. simplicissimum and P. svalbardense have been reported to produce xanthoepocin [24, 28]. Even though andrastin A, penicilllic acid, and xanthoepocin have been found in species outside section Lanata-Divaricata [15] the particular combination of these extrolites is mostly found in this section. P. excelsum produces a profile of extrolites close to that of P. brasilianum, P. ochrochloron, P. pulvillorum and P. rolfsii and the close relationship is confirmed by sequence and morphological data as shown in Figs 1, 2 and 3.


Penicillium excelsum sp. nov from the Brazil Nut Tree Ecosystem in the Amazon Basin'.

Taniwaki MH, Pitt JI, Iamanaka BT, Massi FP, Fungaro MH, Frisvad JC - PLoS ONE (2015)

Maximum-likelihood tree reconstructed from the partial β-tubulin (BenA) gene sequences aligned with corresponding sequences of Penicillium section Lanata-Divaricata deposited in public databases.Numbers at branch nodes refer to bootstrap values (1,000 replicates), only values of >70% are shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696661&req=5

pone.0143189.g002: Maximum-likelihood tree reconstructed from the partial β-tubulin (BenA) gene sequences aligned with corresponding sequences of Penicillium section Lanata-Divaricata deposited in public databases.Numbers at branch nodes refer to bootstrap values (1,000 replicates), only values of >70% are shown.
Mentions: HPLC-DAD analysis of extracts showed that several strains of P. excelsum produce andrastin A, penicillic acid, while some also produce xanthoepocin. Strain ITAL 3000 also produced curvulic acid. Related species also produce penicillic acid, for example P. brasilianum, P. cremeogriseum, P. ochrochloron P. pulvillorum and P. vanderhammenii [24, 26, 27]. P. pulvillorum and P. simplicissimum have also been reported to produce andrastin A, and P. brasilianum, P. ochrochloron, P. pulvillorum, P. rolfsii, P. simplicissimum and P. svalbardense have been reported to produce xanthoepocin [24, 28]. Even though andrastin A, penicilllic acid, and xanthoepocin have been found in species outside section Lanata-Divaricata [15] the particular combination of these extrolites is mostly found in this section. P. excelsum produces a profile of extrolites close to that of P. brasilianum, P. ochrochloron, P. pulvillorum and P. rolfsii and the close relationship is confirmed by sequence and morphological data as shown in Figs 1, 2 and 3.

Bottom Line: It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest.The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences.The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciência e Qualidade de Alimentos, Instituto de Tecnologia de Alimentos, Campinas, São Paulo, Brazil.

ABSTRACT
A new Penicillium species, P. excelsum, is described here using morphological characters, extrolite and partial sequence data from the ITS, β-tubulin and calmodulin genes. It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest. The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences. The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype.

Show MeSH