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Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice.

Cao W, Li L, Tran B, Kajiura S, Amoh Y, Liu F, Hoffman RM - PLoS ONE (2015)

Bottom Line: DMSO better cryopreserved mouse whisker follicles compared to glycerol.Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression.Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer Inc., San Diego, CA, 92111, United States of America.

ABSTRACT
We previously demonstrated that whole hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker hair follicles maintain their hair growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.

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Related in: MedlinePlus

In vivo images comparing hair-shaft growth in fresh follicles and in DMSO cryopreservated follicles after subcutaneous transplantation.At 2 weeks after transplantation, fresh and DSMO-cryopreserved hair follicles established blood-vessel connections with the host nude mice, indicated by red blood in the lower and upper parts of the hair follicle. At week 4, there were more blood vessels surrounding the hair follicles. There were obvious hair roots in the bulb area and longer hair shafts at 4 weeks compared to 2 weeks, indicating the cryopreserved hair follicles started to grow hair shafts compared to fresh follicles. At 6 weeks, more hair follicles had hair roots in the bulb area and grew out long hair shafts in the transplanted fresh and cryopreserved hair follicles. At 8 weeks, there was a large number of long hair shafts. Bars = 1 mm.
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pone.0145997.g004: In vivo images comparing hair-shaft growth in fresh follicles and in DMSO cryopreservated follicles after subcutaneous transplantation.At 2 weeks after transplantation, fresh and DSMO-cryopreserved hair follicles established blood-vessel connections with the host nude mice, indicated by red blood in the lower and upper parts of the hair follicle. At week 4, there were more blood vessels surrounding the hair follicles. There were obvious hair roots in the bulb area and longer hair shafts at 4 weeks compared to 2 weeks, indicating the cryopreserved hair follicles started to grow hair shafts compared to fresh follicles. At 6 weeks, more hair follicles had hair roots in the bulb area and grew out long hair shafts in the transplanted fresh and cryopreserved hair follicles. At 8 weeks, there was a large number of long hair shafts. Bars = 1 mm.

Mentions: In fresh hair follicles, the hair follicle established blood vessel connection with the host nude mice and grew rapidly by week 2 (1.26 mm ± 0.15mm). At week 4, the average of the 10 longest hair shafts length was 3.82 mm ± 0.31 mm. At week 6, the average of the 10 longest hair shafts length was 5.73 mm ± 0.31 mm), and at week 8, the hair shafts length was 7.66 mm ± 0.63 mm. The DSMO-cryopreserved hair follicle also established blood vessel connections with the host nude mice as indicated by red blood in the lower part and upper part of the hair follicle. At week 4, there were more blood vessels surrounding the hair follicles. There were obvious hair roots at the bulb area as well as longer hair shafts shaft at week 4, with the longest length of 1.88 mm ± 0.55 mm, compared to week 2, when the longest hair shaft length was 0.85 mm ± 0.07 mm (Fig 4). These results indicated that the DSMO-cryopreserved hair follicle started to rapidly regrow hair. At week 6, the longest hair shafts grew up to 2.85 mm ± 0.66 mm and more hair roots in the bulb area were observed, as well as long hair shafts growing out. Eventually, at week 8, there were a large amount of long hair shafts with the longest at a length of 4.92 mm ± 0.97 mm (Fig 5). Our results showed that DSMO-cryopreserved hair follicles maintained the ability to produce hair shafts after subcutaneous transplantation.


Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice.

Cao W, Li L, Tran B, Kajiura S, Amoh Y, Liu F, Hoffman RM - PLoS ONE (2015)

In vivo images comparing hair-shaft growth in fresh follicles and in DMSO cryopreservated follicles after subcutaneous transplantation.At 2 weeks after transplantation, fresh and DSMO-cryopreserved hair follicles established blood-vessel connections with the host nude mice, indicated by red blood in the lower and upper parts of the hair follicle. At week 4, there were more blood vessels surrounding the hair follicles. There were obvious hair roots in the bulb area and longer hair shafts at 4 weeks compared to 2 weeks, indicating the cryopreserved hair follicles started to grow hair shafts compared to fresh follicles. At 6 weeks, more hair follicles had hair roots in the bulb area and grew out long hair shafts in the transplanted fresh and cryopreserved hair follicles. At 8 weeks, there was a large number of long hair shafts. Bars = 1 mm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696652&req=5

pone.0145997.g004: In vivo images comparing hair-shaft growth in fresh follicles and in DMSO cryopreservated follicles after subcutaneous transplantation.At 2 weeks after transplantation, fresh and DSMO-cryopreserved hair follicles established blood-vessel connections with the host nude mice, indicated by red blood in the lower and upper parts of the hair follicle. At week 4, there were more blood vessels surrounding the hair follicles. There were obvious hair roots in the bulb area and longer hair shafts at 4 weeks compared to 2 weeks, indicating the cryopreserved hair follicles started to grow hair shafts compared to fresh follicles. At 6 weeks, more hair follicles had hair roots in the bulb area and grew out long hair shafts in the transplanted fresh and cryopreserved hair follicles. At 8 weeks, there was a large number of long hair shafts. Bars = 1 mm.
Mentions: In fresh hair follicles, the hair follicle established blood vessel connection with the host nude mice and grew rapidly by week 2 (1.26 mm ± 0.15mm). At week 4, the average of the 10 longest hair shafts length was 3.82 mm ± 0.31 mm. At week 6, the average of the 10 longest hair shafts length was 5.73 mm ± 0.31 mm), and at week 8, the hair shafts length was 7.66 mm ± 0.63 mm. The DSMO-cryopreserved hair follicle also established blood vessel connections with the host nude mice as indicated by red blood in the lower part and upper part of the hair follicle. At week 4, there were more blood vessels surrounding the hair follicles. There were obvious hair roots at the bulb area as well as longer hair shafts shaft at week 4, with the longest length of 1.88 mm ± 0.55 mm, compared to week 2, when the longest hair shaft length was 0.85 mm ± 0.07 mm (Fig 4). These results indicated that the DSMO-cryopreserved hair follicle started to rapidly regrow hair. At week 6, the longest hair shafts grew up to 2.85 mm ± 0.66 mm and more hair roots in the bulb area were observed, as well as long hair shafts growing out. Eventually, at week 8, there were a large amount of long hair shafts with the longest at a length of 4.92 mm ± 0.97 mm (Fig 5). Our results showed that DSMO-cryopreserved hair follicles maintained the ability to produce hair shafts after subcutaneous transplantation.

Bottom Line: DMSO better cryopreserved mouse whisker follicles compared to glycerol.Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression.Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer Inc., San Diego, CA, 92111, United States of America.

ABSTRACT
We previously demonstrated that whole hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker hair follicles maintain their hair growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.

Show MeSH
Related in: MedlinePlus