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Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice.

Cao W, Li L, Tran B, Kajiura S, Amoh Y, Liu F, Hoffman RM - PLoS ONE (2015)

Bottom Line: DMSO better cryopreserved mouse whisker follicles compared to glycerol.Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression.Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer Inc., San Diego, CA, 92111, United States of America.

ABSTRACT
We previously demonstrated that whole hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker hair follicles maintain their hair growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.

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Schema of hair follicle isolation and cryopreservation, Gelfoam histocultured and transplantation.
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pone.0145997.g001: Schema of hair follicle isolation and cryopreservation, Gelfoam histocultured and transplantation.

Mentions: Whicker hair follicles were carefully isolated by microdissection from nestin driven (ND)-GFP mice. The intact hair follicle bulb with the attached sensory nerve was gently removed from subctuaneous fat under a stereo dissecting microscope [6, 10, 11]. In order to cryopreserve the hair follicle, fresh isolated whole whisker hair follicles were then transferred to cryovials. Either 10% glycerol or DMSO with 90% FBS was added (1 ml) as the freezing medium (Fig 1). For short time period, cryovials containing hair follicles were stored in an -80°C freezer.


Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice.

Cao W, Li L, Tran B, Kajiura S, Amoh Y, Liu F, Hoffman RM - PLoS ONE (2015)

Schema of hair follicle isolation and cryopreservation, Gelfoam histocultured and transplantation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696652&req=5

pone.0145997.g001: Schema of hair follicle isolation and cryopreservation, Gelfoam histocultured and transplantation.
Mentions: Whicker hair follicles were carefully isolated by microdissection from nestin driven (ND)-GFP mice. The intact hair follicle bulb with the attached sensory nerve was gently removed from subctuaneous fat under a stereo dissecting microscope [6, 10, 11]. In order to cryopreserve the hair follicle, fresh isolated whole whisker hair follicles were then transferred to cryovials. Either 10% glycerol or DMSO with 90% FBS was added (1 ml) as the freezing medium (Fig 1). For short time period, cryovials containing hair follicles were stored in an -80°C freezer.

Bottom Line: DMSO better cryopreserved mouse whisker follicles compared to glycerol.Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression.Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer Inc., San Diego, CA, 92111, United States of America.

ABSTRACT
We previously demonstrated that whole hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker hair follicles maintain their hair growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.

Show MeSH
Related in: MedlinePlus