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Genotoxic capacity of Cd/Se semiconductor quantum dots with differing surface chemistries.

Manshian BB, Soenen SJ, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Mutagenesis (2015)

Bottom Line: However, our understanding of the toxicological structure-activity relationships remains limited.Carboxyl-QD demonstrated the smallest agglomerate size and greatest cellular uptake, which correlated with a dose dependent increase in cytotoxicity and genotoxicity.Oxidative stress and induced mitochondrial reactive oxygen species were only partially associated with cytotoxicity and genotoxicity induced by the QD, hence were not the only mechanisms of importance.

View Article: PubMed Central - PubMed

Affiliation: Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Biomedical NMR Unit-MoSAIC, Department of Medicine, KU Leuven, B-3000 Leuven, Belgium and.

No MeSH data available.


Related in: MedlinePlus

Oxidative stress induced following exposure to QD. (A) Carboxyl-QD, (B) amine-QD, (C) HDA-QD exposed to TK6 cells for 4 or 18h. Line graphs represent ROS, while bar graphs represent mito-ROS. Results are presented as mean ± standard deviation (n = 3). Significance is indicated with *P ≤ 0.05 and **P ≤ 0.005.
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Figure 6: Oxidative stress induced following exposure to QD. (A) Carboxyl-QD, (B) amine-QD, (C) HDA-QD exposed to TK6 cells for 4 or 18h. Line graphs represent ROS, while bar graphs represent mito-ROS. Results are presented as mean ± standard deviation (n = 3). Significance is indicated with *P ≤ 0.05 and **P ≤ 0.005.

Mentions: Having observed significant pockets of genotoxicity following exposure to QD, further experiments were conducted to evaluate the association of oxidative stress in response to QD of different surface chemistries. The production of both ROS and any changes to the mitochondrial membrane potential (Δψm) were examined following 4 and 18h exposure periods. The 4h time point was selected to ensure the detection of early oxidative species; while the later time point represents the cell state at the end of the exposure period. Carboxyl- and amine-QD did not result in the significant induction of ROS in TK6 cells following either 4 or 18h exposure (Figure 6). However, HDA-QD demonstrated a clear increase in ROS levels, at all the tested concentrations up to 10nM, following treatment for 4h (Figure 6C dark line). These ROS elevations were diminished after 18h exposure.


Genotoxic capacity of Cd/Se semiconductor quantum dots with differing surface chemistries.

Manshian BB, Soenen SJ, Brown A, Hondow N, Wills J, Jenkins GJ, Doak SH - Mutagenesis (2015)

Oxidative stress induced following exposure to QD. (A) Carboxyl-QD, (B) amine-QD, (C) HDA-QD exposed to TK6 cells for 4 or 18h. Line graphs represent ROS, while bar graphs represent mito-ROS. Results are presented as mean ± standard deviation (n = 3). Significance is indicated with *P ≤ 0.05 and **P ≤ 0.005.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4696518&req=5

Figure 6: Oxidative stress induced following exposure to QD. (A) Carboxyl-QD, (B) amine-QD, (C) HDA-QD exposed to TK6 cells for 4 or 18h. Line graphs represent ROS, while bar graphs represent mito-ROS. Results are presented as mean ± standard deviation (n = 3). Significance is indicated with *P ≤ 0.05 and **P ≤ 0.005.
Mentions: Having observed significant pockets of genotoxicity following exposure to QD, further experiments were conducted to evaluate the association of oxidative stress in response to QD of different surface chemistries. The production of both ROS and any changes to the mitochondrial membrane potential (Δψm) were examined following 4 and 18h exposure periods. The 4h time point was selected to ensure the detection of early oxidative species; while the later time point represents the cell state at the end of the exposure period. Carboxyl- and amine-QD did not result in the significant induction of ROS in TK6 cells following either 4 or 18h exposure (Figure 6). However, HDA-QD demonstrated a clear increase in ROS levels, at all the tested concentrations up to 10nM, following treatment for 4h (Figure 6C dark line). These ROS elevations were diminished after 18h exposure.

Bottom Line: However, our understanding of the toxicological structure-activity relationships remains limited.Carboxyl-QD demonstrated the smallest agglomerate size and greatest cellular uptake, which correlated with a dose dependent increase in cytotoxicity and genotoxicity.Oxidative stress and induced mitochondrial reactive oxygen species were only partially associated with cytotoxicity and genotoxicity induced by the QD, hence were not the only mechanisms of importance.

View Article: PubMed Central - PubMed

Affiliation: Institute of Life Science, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK, Biomedical NMR Unit-MoSAIC, Department of Medicine, KU Leuven, B-3000 Leuven, Belgium and.

No MeSH data available.


Related in: MedlinePlus