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Evaluation of Glutathione-S-Transferase P1 Polymorphism and its Relation to Bone Mineral Density in Egyptian Children and Adolescents with Beta-Thalassemia Major.

Ragab SM, Badr EA, Ibrahim AS - Mediterr J Hematol Infect Dis (2016)

Bottom Line: They exhibited higher osteocalcin levels compared to heterozygotes and wild homozygotes (p=0.012, p=0.013, respectively).Ile105Val polymorphism was frequent among TM patients and could increase their susceptibility to reduced BMD.Large sample studies are required to confirm these findings.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pediatrics, Faculty of Medicine, Menoufia University, NaserStreet, Shebeen El-koom, Menoufia, Egypt.

ABSTRACT

Background: Osteoporosis is a major complication of beta thalassemia major (TM). Increased oxidative stress and its controlling genes were linked to osteoporosis. Ile105 Val variant is a functional polymorphism of Glutathione S-transferase P1 (GSTP1), with reduced anti-oxidative property. No data are available about this variant or its association with osteoporosis among thalassemia patients yet.

Objectives: To investigate Ile105Val polymorphism and its possible association with bone mineral density (BMD) values in a group of TM children.

Methods: Thirty five TM children and 30 age and sex matched healthy controls were included. Liver and renal functions, serum ferritin, calcium, phosphorous, alkaline phosphatase and osteocalcin were assayed. BMD was determined by DXA with calculation of Z-scores at lumbar spine (LS) and femoral neck (FN). Height for age Z- score (HAZ) adjusted BMD Z-scores were calculated. GSTP1 Ile105Val polymorphism was studied by polymerase chain reaction-restriction fragment length polymorphism.

Results: The relative frequency of 105 Val allele was significantly higher in TM patients than the controls (p<0.0001). Significant association between genotype subgroups and BMD parameters was detected. Compared to wild homozygotes, polymorphic homozygotes had lower LS-BMD (p =0.029), LS-BMD Z -score (p=0.008 ), LS- BMD haz - Z-score (p=0.011), FN- BMD (p= 0.001), FN- BMD Z -score (p=0.02) and FN-BMD haz - Z-score (p=0.001). They exhibited higher osteocalcin levels compared to heterozygotes and wild homozygotes (p=0.012, p=0.013, respectively).

Conclusion: Ile105Val polymorphism was frequent among TM patients and could increase their susceptibility to reduced BMD. Large sample studies are required to confirm these findings.

No MeSH data available.


Related in: MedlinePlus

A. Correlation between BMD Z score LS and the mean yearly serum ferritin among the patient group. B. Correlation between BMD HAZ -Z LS and the mean yearly serum ferritin among the patient group.
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f2-mjhid-8-1-e2016004: A. Correlation between BMD Z score LS and the mean yearly serum ferritin among the patient group. B. Correlation between BMD HAZ -Z LS and the mean yearly serum ferritin among the patient group.

Mentions: Both BMD Z-score at LS and its HAZ adjusted values had significant inverse correlation with serum ferritin (r = −0.525, p= 0.001 and r= −0.433, p= 0.009, respectively; Figure 2). Both FN-BMD Z-score and its HAZ adjusted values had a negative correlation trend with serum ferritin (r= −0.33, p=0.05).


Evaluation of Glutathione-S-Transferase P1 Polymorphism and its Relation to Bone Mineral Density in Egyptian Children and Adolescents with Beta-Thalassemia Major.

Ragab SM, Badr EA, Ibrahim AS - Mediterr J Hematol Infect Dis (2016)

A. Correlation between BMD Z score LS and the mean yearly serum ferritin among the patient group. B. Correlation between BMD HAZ -Z LS and the mean yearly serum ferritin among the patient group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696466&req=5

f2-mjhid-8-1-e2016004: A. Correlation between BMD Z score LS and the mean yearly serum ferritin among the patient group. B. Correlation between BMD HAZ -Z LS and the mean yearly serum ferritin among the patient group.
Mentions: Both BMD Z-score at LS and its HAZ adjusted values had significant inverse correlation with serum ferritin (r = −0.525, p= 0.001 and r= −0.433, p= 0.009, respectively; Figure 2). Both FN-BMD Z-score and its HAZ adjusted values had a negative correlation trend with serum ferritin (r= −0.33, p=0.05).

Bottom Line: They exhibited higher osteocalcin levels compared to heterozygotes and wild homozygotes (p=0.012, p=0.013, respectively).Ile105Val polymorphism was frequent among TM patients and could increase their susceptibility to reduced BMD.Large sample studies are required to confirm these findings.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pediatrics, Faculty of Medicine, Menoufia University, NaserStreet, Shebeen El-koom, Menoufia, Egypt.

ABSTRACT

Background: Osteoporosis is a major complication of beta thalassemia major (TM). Increased oxidative stress and its controlling genes were linked to osteoporosis. Ile105 Val variant is a functional polymorphism of Glutathione S-transferase P1 (GSTP1), with reduced anti-oxidative property. No data are available about this variant or its association with osteoporosis among thalassemia patients yet.

Objectives: To investigate Ile105Val polymorphism and its possible association with bone mineral density (BMD) values in a group of TM children.

Methods: Thirty five TM children and 30 age and sex matched healthy controls were included. Liver and renal functions, serum ferritin, calcium, phosphorous, alkaline phosphatase and osteocalcin were assayed. BMD was determined by DXA with calculation of Z-scores at lumbar spine (LS) and femoral neck (FN). Height for age Z- score (HAZ) adjusted BMD Z-scores were calculated. GSTP1 Ile105Val polymorphism was studied by polymerase chain reaction-restriction fragment length polymorphism.

Results: The relative frequency of 105 Val allele was significantly higher in TM patients than the controls (p<0.0001). Significant association between genotype subgroups and BMD parameters was detected. Compared to wild homozygotes, polymorphic homozygotes had lower LS-BMD (p =0.029), LS-BMD Z -score (p=0.008 ), LS- BMD haz - Z-score (p=0.011), FN- BMD (p= 0.001), FN- BMD Z -score (p=0.02) and FN-BMD haz - Z-score (p=0.001). They exhibited higher osteocalcin levels compared to heterozygotes and wild homozygotes (p=0.012, p=0.013, respectively).

Conclusion: Ile105Val polymorphism was frequent among TM patients and could increase their susceptibility to reduced BMD. Large sample studies are required to confirm these findings.

No MeSH data available.


Related in: MedlinePlus