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Planarians as a model of aging to study the interaction between stem cells and senescent cells in vivo.

Perrigue PM, Najbauer J, Jozwiak AA, Barciszewski J, Aboody KS, Barish ME - Pathobiol Aging Age Relat Dis (2015)

Bottom Line: The depletion of stem cell pools and the accumulation of senescent cells in animal tissues are linked to aging.Planarians are invertebrate flatworms and are unusual in that their stem cells, called neoblasts, are constantly replacing old and dying cells.By eliminating neoblasts in worms via irradiation, the biological principles of aging are exposed in the absence of wound healing and regeneration, making planaria a powerful tool for aging research.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland; pperrigue@ibch.poznan.pl.

ABSTRACT
The depletion of stem cell pools and the accumulation of senescent cells in animal tissues are linked to aging. Planarians are invertebrate flatworms and are unusual in that their stem cells, called neoblasts, are constantly replacing old and dying cells. By eliminating neoblasts in worms via irradiation, the biological principles of aging are exposed in the absence of wound healing and regeneration, making planaria a powerful tool for aging research.

No MeSH data available.


Related in: MedlinePlus

(A) Western blot of H3S10P, H3K27me3, and β-actin. The following commercially available antibodies were used at the indicated concentrations: H3S10P (05-806, Millipore, 1:50,000), H3K27me3 (GTX12184, Genetex, 1:1,000), and β-actin (GTX109639, Genetex, 1:12,500). Lysates for western blotting were made from five worms that were collected at each time point. (B) SA-β-gal activity staining in irradiated worm tissues. Black arrows point to positive staining for SA-β-gal activity.
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Figure 0002: (A) Western blot of H3S10P, H3K27me3, and β-actin. The following commercially available antibodies were used at the indicated concentrations: H3S10P (05-806, Millipore, 1:50,000), H3K27me3 (GTX12184, Genetex, 1:1,000), and β-actin (GTX109639, Genetex, 1:12,500). Lysates for western blotting were made from five worms that were collected at each time point. (B) SA-β-gal activity staining in irradiated worm tissues. Black arrows point to positive staining for SA-β-gal activity.

Mentions: We next investigated histone modifications related to stem cells and aging in a similar time-course experiment. Neoblasts are the only dividing cells in planarians and can be tracked using the proliferation marker phospho-histone H3 serine 10 (H3S10P) (17). Western blot analysis indicated the complete elimination of neoblasts by 3 and 5 dpi, followed by a decrease in H3K27me3 levels by 5 and 7 dpi (Fig. 2A). These data suggest that depletion of neoblasts results in accumulation of senescent cells in irradiated worms.


Planarians as a model of aging to study the interaction between stem cells and senescent cells in vivo.

Perrigue PM, Najbauer J, Jozwiak AA, Barciszewski J, Aboody KS, Barish ME - Pathobiol Aging Age Relat Dis (2015)

(A) Western blot of H3S10P, H3K27me3, and β-actin. The following commercially available antibodies were used at the indicated concentrations: H3S10P (05-806, Millipore, 1:50,000), H3K27me3 (GTX12184, Genetex, 1:1,000), and β-actin (GTX109639, Genetex, 1:12,500). Lysates for western blotting were made from five worms that were collected at each time point. (B) SA-β-gal activity staining in irradiated worm tissues. Black arrows point to positive staining for SA-β-gal activity.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696462&req=5

Figure 0002: (A) Western blot of H3S10P, H3K27me3, and β-actin. The following commercially available antibodies were used at the indicated concentrations: H3S10P (05-806, Millipore, 1:50,000), H3K27me3 (GTX12184, Genetex, 1:1,000), and β-actin (GTX109639, Genetex, 1:12,500). Lysates for western blotting were made from five worms that were collected at each time point. (B) SA-β-gal activity staining in irradiated worm tissues. Black arrows point to positive staining for SA-β-gal activity.
Mentions: We next investigated histone modifications related to stem cells and aging in a similar time-course experiment. Neoblasts are the only dividing cells in planarians and can be tracked using the proliferation marker phospho-histone H3 serine 10 (H3S10P) (17). Western blot analysis indicated the complete elimination of neoblasts by 3 and 5 dpi, followed by a decrease in H3K27me3 levels by 5 and 7 dpi (Fig. 2A). These data suggest that depletion of neoblasts results in accumulation of senescent cells in irradiated worms.

Bottom Line: The depletion of stem cell pools and the accumulation of senescent cells in animal tissues are linked to aging.Planarians are invertebrate flatworms and are unusual in that their stem cells, called neoblasts, are constantly replacing old and dying cells.By eliminating neoblasts in worms via irradiation, the biological principles of aging are exposed in the absence of wound healing and regeneration, making planaria a powerful tool for aging research.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland; pperrigue@ibch.poznan.pl.

ABSTRACT
The depletion of stem cell pools and the accumulation of senescent cells in animal tissues are linked to aging. Planarians are invertebrate flatworms and are unusual in that their stem cells, called neoblasts, are constantly replacing old and dying cells. By eliminating neoblasts in worms via irradiation, the biological principles of aging are exposed in the absence of wound healing and regeneration, making planaria a powerful tool for aging research.

No MeSH data available.


Related in: MedlinePlus