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Planarians as a model of aging to study the interaction between stem cells and senescent cells in vivo.

Perrigue PM, Najbauer J, Jozwiak AA, Barciszewski J, Aboody KS, Barish ME - Pathobiol Aging Age Relat Dis (2015)

Bottom Line: The depletion of stem cell pools and the accumulation of senescent cells in animal tissues are linked to aging.Planarians are invertebrate flatworms and are unusual in that their stem cells, called neoblasts, are constantly replacing old and dying cells.By eliminating neoblasts in worms via irradiation, the biological principles of aging are exposed in the absence of wound healing and regeneration, making planaria a powerful tool for aging research.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland; pperrigue@ibch.poznan.pl.

ABSTRACT
The depletion of stem cell pools and the accumulation of senescent cells in animal tissues are linked to aging. Planarians are invertebrate flatworms and are unusual in that their stem cells, called neoblasts, are constantly replacing old and dying cells. By eliminating neoblasts in worms via irradiation, the biological principles of aging are exposed in the absence of wound healing and regeneration, making planaria a powerful tool for aging research.

No MeSH data available.


Related in: MedlinePlus

Images of planaria that were γ-irradiated using a 137Cs source (100 Gy).
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Figure 0001: Images of planaria that were γ-irradiated using a 137Cs source (100 Gy).

Mentions: To induce aging, worms were treated with one dose of radiation (100 Gy) and subsequently imaged for 10 days (Fig. 1). Irradiated worms appeared phenotypically healthy at 1 and 3 days post irradiation (dpi), followed by a gradual decrease in body size and appearance of lesions at 5 and 7 dpi. All of the irradiated worms lysed by 10 dpi due to inability to maintain tissue homeostasis and eventually all died at 14 dpi (n=10). Similar to previous reports, the physical appearance, body mass, and size of the 10 dpi worm compared to the untreated control in Fig. 1 suggest that radiation treatment triggered large amounts of apoptotic cell death (15). In planarians, apoptosis of differentiated cells has been reported during regeneration and remodeling of preexisting tissues in planarians (16). It is unknown if the status of some of the remaining cell types that evade apoptosis reprogram to cellular senescence and contribute to the process of radiation-induced aging in planarians.


Planarians as a model of aging to study the interaction between stem cells and senescent cells in vivo.

Perrigue PM, Najbauer J, Jozwiak AA, Barciszewski J, Aboody KS, Barish ME - Pathobiol Aging Age Relat Dis (2015)

Images of planaria that were γ-irradiated using a 137Cs source (100 Gy).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4696462&req=5

Figure 0001: Images of planaria that were γ-irradiated using a 137Cs source (100 Gy).
Mentions: To induce aging, worms were treated with one dose of radiation (100 Gy) and subsequently imaged for 10 days (Fig. 1). Irradiated worms appeared phenotypically healthy at 1 and 3 days post irradiation (dpi), followed by a gradual decrease in body size and appearance of lesions at 5 and 7 dpi. All of the irradiated worms lysed by 10 dpi due to inability to maintain tissue homeostasis and eventually all died at 14 dpi (n=10). Similar to previous reports, the physical appearance, body mass, and size of the 10 dpi worm compared to the untreated control in Fig. 1 suggest that radiation treatment triggered large amounts of apoptotic cell death (15). In planarians, apoptosis of differentiated cells has been reported during regeneration and remodeling of preexisting tissues in planarians (16). It is unknown if the status of some of the remaining cell types that evade apoptosis reprogram to cellular senescence and contribute to the process of radiation-induced aging in planarians.

Bottom Line: The depletion of stem cell pools and the accumulation of senescent cells in animal tissues are linked to aging.Planarians are invertebrate flatworms and are unusual in that their stem cells, called neoblasts, are constantly replacing old and dying cells.By eliminating neoblasts in worms via irradiation, the biological principles of aging are exposed in the absence of wound healing and regeneration, making planaria a powerful tool for aging research.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland; pperrigue@ibch.poznan.pl.

ABSTRACT
The depletion of stem cell pools and the accumulation of senescent cells in animal tissues are linked to aging. Planarians are invertebrate flatworms and are unusual in that their stem cells, called neoblasts, are constantly replacing old and dying cells. By eliminating neoblasts in worms via irradiation, the biological principles of aging are exposed in the absence of wound healing and regeneration, making planaria a powerful tool for aging research.

No MeSH data available.


Related in: MedlinePlus