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Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster).

Assefa S, Ahles K, Bigelow S, Curtis JT, Köhler GA - Gut Pathog (2015)

Bottom Line: The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior.This study demonstrates that lactobacilli with probiotic potential are present in the vole intestine.The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Microbiology, Oklahoma State University Center for Health Sciences, 1111 West 17th Street, Tulsa, OK 74107 USA.

ABSTRACT

Background: Recent research suggests integration of the intestinal microbiota in gut-brain communication which could lead to new approaches to treat neurological disorders. The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior. For future studies on the role of probiotics in ameliorating disorders with social withdrawal symptoms, we report the characterization of intestinal Lactobacillus isolates with probiotic potential from voles.

Methods and results: 30 bacterial strains were isolated from the vole intestine and found to be distinct but closely related to Lactobacillus johnsonii using 16S rRNA gene sequencing and Random Amplification of Polymorphic DNA fingerprinting. In vitro characterizations including acid and bile tolerance, antimicrobial effects, antibiotic susceptibility, and adherence to intestinal epithelial cells were performed to assess the probiotic potential of selected strains. Since previous studies revealed that mercury ingestion triggers social deficits in voles, mercury resistance of the probiotic candidates was evaluated which could be an important factor in preventing/treating these behavioral changes.

Conclusions: This study demonstrates that lactobacilli with probiotic potential are present in the vole intestine. The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.

No MeSH data available.


Related in: MedlinePlus

Biofilm formation on an abiotic surface. The Lactobacillus strains were incubated at 37 °C for 48 h in polystyrene culture dishes containing MRS medium. Biofilm formation was quantified using the crystal violet staining method. Error bars indicate standard deviations of three experiments with triplicate measurements
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Fig7: Biofilm formation on an abiotic surface. The Lactobacillus strains were incubated at 37 °C for 48 h in polystyrene culture dishes containing MRS medium. Biofilm formation was quantified using the crystal violet staining method. Error bars indicate standard deviations of three experiments with triplicate measurements

Mentions: A crystal violet staining assay [42] was employed to test the 30 Lactobacillus strains for biofilm formation in tissue culture plates. Results are shown in Fig. 7. The assay revealed a wide range of variation in biofilm formation among the strains with statistically significant differences (P < 0.0001). Strain PV036 showed the highest biofilm production, whereas PV031 and PV037 were the lowest biofilm producers.Fig. 7


Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster).

Assefa S, Ahles K, Bigelow S, Curtis JT, Köhler GA - Gut Pathog (2015)

Biofilm formation on an abiotic surface. The Lactobacillus strains were incubated at 37 °C for 48 h in polystyrene culture dishes containing MRS medium. Biofilm formation was quantified using the crystal violet staining method. Error bars indicate standard deviations of three experiments with triplicate measurements
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4696317&req=5

Fig7: Biofilm formation on an abiotic surface. The Lactobacillus strains were incubated at 37 °C for 48 h in polystyrene culture dishes containing MRS medium. Biofilm formation was quantified using the crystal violet staining method. Error bars indicate standard deviations of three experiments with triplicate measurements
Mentions: A crystal violet staining assay [42] was employed to test the 30 Lactobacillus strains for biofilm formation in tissue culture plates. Results are shown in Fig. 7. The assay revealed a wide range of variation in biofilm formation among the strains with statistically significant differences (P < 0.0001). Strain PV036 showed the highest biofilm production, whereas PV031 and PV037 were the lowest biofilm producers.Fig. 7

Bottom Line: The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior.This study demonstrates that lactobacilli with probiotic potential are present in the vole intestine.The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Microbiology, Oklahoma State University Center for Health Sciences, 1111 West 17th Street, Tulsa, OK 74107 USA.

ABSTRACT

Background: Recent research suggests integration of the intestinal microbiota in gut-brain communication which could lead to new approaches to treat neurological disorders. The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior. For future studies on the role of probiotics in ameliorating disorders with social withdrawal symptoms, we report the characterization of intestinal Lactobacillus isolates with probiotic potential from voles.

Methods and results: 30 bacterial strains were isolated from the vole intestine and found to be distinct but closely related to Lactobacillus johnsonii using 16S rRNA gene sequencing and Random Amplification of Polymorphic DNA fingerprinting. In vitro characterizations including acid and bile tolerance, antimicrobial effects, antibiotic susceptibility, and adherence to intestinal epithelial cells were performed to assess the probiotic potential of selected strains. Since previous studies revealed that mercury ingestion triggers social deficits in voles, mercury resistance of the probiotic candidates was evaluated which could be an important factor in preventing/treating these behavioral changes.

Conclusions: This study demonstrates that lactobacilli with probiotic potential are present in the vole intestine. The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.

No MeSH data available.


Related in: MedlinePlus