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Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster).

Assefa S, Ahles K, Bigelow S, Curtis JT, Köhler GA - Gut Pathog (2015)

Bottom Line: The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior.This study demonstrates that lactobacilli with probiotic potential are present in the vole intestine.The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Microbiology, Oklahoma State University Center for Health Sciences, 1111 West 17th Street, Tulsa, OK 74107 USA.

ABSTRACT

Background: Recent research suggests integration of the intestinal microbiota in gut-brain communication which could lead to new approaches to treat neurological disorders. The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior. For future studies on the role of probiotics in ameliorating disorders with social withdrawal symptoms, we report the characterization of intestinal Lactobacillus isolates with probiotic potential from voles.

Methods and results: 30 bacterial strains were isolated from the vole intestine and found to be distinct but closely related to Lactobacillus johnsonii using 16S rRNA gene sequencing and Random Amplification of Polymorphic DNA fingerprinting. In vitro characterizations including acid and bile tolerance, antimicrobial effects, antibiotic susceptibility, and adherence to intestinal epithelial cells were performed to assess the probiotic potential of selected strains. Since previous studies revealed that mercury ingestion triggers social deficits in voles, mercury resistance of the probiotic candidates was evaluated which could be an important factor in preventing/treating these behavioral changes.

Conclusions: This study demonstrates that lactobacilli with probiotic potential are present in the vole intestine. The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.

No MeSH data available.


Related in: MedlinePlus

Random amplified polymorphic DNA (RAPD) analysis of 30 prairie vole Lactobacillus isolates. Dendrogram of the cluster analysis of RAPD results using the DendroUPGMA program (see “Methods”). Putative clustering is indicated with roman numerals. Strains with the overall best performance in this study are boxed in red
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Fig2: Random amplified polymorphic DNA (RAPD) analysis of 30 prairie vole Lactobacillus isolates. Dendrogram of the cluster analysis of RAPD results using the DendroUPGMA program (see “Methods”). Putative clustering is indicated with roman numerals. Strains with the overall best performance in this study are boxed in red

Mentions: Due to their close relatedness, a RAPD typing technique was employed to genetically type the 30 prairie vole Lactobacillus isolates. To systematically examine the genetic fingerprints of the different strains, a set of three previously published RAPD primers (272, 277, and 287; [39]) was evaluated for differentiation of the bacterial strains. Primer 272 (see Table 2) was chosen for further analyses because it delivered the best discriminatory power by reproducibly amplifying five or more random DNA fragments ranging in size from approximately 180 bp to 3000 bp (Fig. 1). Twenty-seven of the 30 isolates share common bands at 175, 375, 1200 and 1500 bp (Fig. 1). In this regard, the RAPD fingerprinting was able to cluster genetically identical strains as well as differentiate distinct strains among the isolates. For instance, multiple strains such as PV010, PV014-PV019 or PV011, PV021, PV023, PV024, PV026, PV027, PV031, PV033, PV036, PV038 and PV039 were found to possess identical RAPD fingerprints suggesting that the isolates were identical or if genetic heterogeneity exists among these isolates, it could not be discriminated by RAPD. Overall, RAPD analysis of the 30 isolates revealed nine distinct clusters (Fig. 2). Notably, eight strains (PV012, PV013, PV020, PV029, PV030, PV032, PV034, and PV035) produced patterns with unique PCR bands (Fig. 2). RAPD bands at 1200, 650, 450, and 300 bp are shared with the human L. johnsonii ATCC 33200 strain by 23, 8, 11, and 12 isolates, respectively. In general, the RAPD fingerprinting analysis was effective for rapid differentiation within the different isolates. L. rhamnosus GG was included as reference strain and showed almost no RAPD pattern similarities to the vole intestinal strains.Table 2


Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster).

Assefa S, Ahles K, Bigelow S, Curtis JT, Köhler GA - Gut Pathog (2015)

Random amplified polymorphic DNA (RAPD) analysis of 30 prairie vole Lactobacillus isolates. Dendrogram of the cluster analysis of RAPD results using the DendroUPGMA program (see “Methods”). Putative clustering is indicated with roman numerals. Strains with the overall best performance in this study are boxed in red
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4696317&req=5

Fig2: Random amplified polymorphic DNA (RAPD) analysis of 30 prairie vole Lactobacillus isolates. Dendrogram of the cluster analysis of RAPD results using the DendroUPGMA program (see “Methods”). Putative clustering is indicated with roman numerals. Strains with the overall best performance in this study are boxed in red
Mentions: Due to their close relatedness, a RAPD typing technique was employed to genetically type the 30 prairie vole Lactobacillus isolates. To systematically examine the genetic fingerprints of the different strains, a set of three previously published RAPD primers (272, 277, and 287; [39]) was evaluated for differentiation of the bacterial strains. Primer 272 (see Table 2) was chosen for further analyses because it delivered the best discriminatory power by reproducibly amplifying five or more random DNA fragments ranging in size from approximately 180 bp to 3000 bp (Fig. 1). Twenty-seven of the 30 isolates share common bands at 175, 375, 1200 and 1500 bp (Fig. 1). In this regard, the RAPD fingerprinting was able to cluster genetically identical strains as well as differentiate distinct strains among the isolates. For instance, multiple strains such as PV010, PV014-PV019 or PV011, PV021, PV023, PV024, PV026, PV027, PV031, PV033, PV036, PV038 and PV039 were found to possess identical RAPD fingerprints suggesting that the isolates were identical or if genetic heterogeneity exists among these isolates, it could not be discriminated by RAPD. Overall, RAPD analysis of the 30 isolates revealed nine distinct clusters (Fig. 2). Notably, eight strains (PV012, PV013, PV020, PV029, PV030, PV032, PV034, and PV035) produced patterns with unique PCR bands (Fig. 2). RAPD bands at 1200, 650, 450, and 300 bp are shared with the human L. johnsonii ATCC 33200 strain by 23, 8, 11, and 12 isolates, respectively. In general, the RAPD fingerprinting analysis was effective for rapid differentiation within the different isolates. L. rhamnosus GG was included as reference strain and showed almost no RAPD pattern similarities to the vole intestinal strains.Table 2

Bottom Line: The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior.This study demonstrates that lactobacilli with probiotic potential are present in the vole intestine.The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Microbiology, Oklahoma State University Center for Health Sciences, 1111 West 17th Street, Tulsa, OK 74107 USA.

ABSTRACT

Background: Recent research suggests integration of the intestinal microbiota in gut-brain communication which could lead to new approaches to treat neurological disorders. The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior. For future studies on the role of probiotics in ameliorating disorders with social withdrawal symptoms, we report the characterization of intestinal Lactobacillus isolates with probiotic potential from voles.

Methods and results: 30 bacterial strains were isolated from the vole intestine and found to be distinct but closely related to Lactobacillus johnsonii using 16S rRNA gene sequencing and Random Amplification of Polymorphic DNA fingerprinting. In vitro characterizations including acid and bile tolerance, antimicrobial effects, antibiotic susceptibility, and adherence to intestinal epithelial cells were performed to assess the probiotic potential of selected strains. Since previous studies revealed that mercury ingestion triggers social deficits in voles, mercury resistance of the probiotic candidates was evaluated which could be an important factor in preventing/treating these behavioral changes.

Conclusions: This study demonstrates that lactobacilli with probiotic potential are present in the vole intestine. The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.

No MeSH data available.


Related in: MedlinePlus