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Antibacterial activities of the methanol extracts and compounds from Erythrina sigmoidea against Gram-negative multi-drug resistant phenotypes.

Djeussi DE, Sandjo LP, Noumedem JA, Omosa LK, T Ngadjui B, Kuete V - BMC Complement Altern Med (2015)

Bottom Line: The MIC results indicated that the crude extracts from the leaves and bark of this plant were able to inhibit the growth of 96.3 % of the 27 tested bacteria.Compounds 2-6 displayed selective activities, their inhibitory effects being obtained on 8.3 %, 41.7 %, 58.3 %, 58.3 % and 66.7 % of tested bacteria respectively for 2, 3, 5, 6 and 4.Neobavaisoflavone (6) is the main antibacterial consituents of the bark crude extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT

Background: In the present study, the methanol extracts from the leaves, as well as compounds namely sigmoidin I (1), atalantoflavone (2), bidwillon A (3), neocyclomorusin (4), 6α-hydroxyphaseollidin (5) and neobavaisoflavone (6) (from the bark extract) were tested for their activities against a panel of Gram-negative bacteria including multi-drug resistant (MDR) phenotypes.

Methods: Broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the extracts as well as compounds 1-6.

Results: The MIC results indicated that the crude extracts from the leaves and bark of this plant were able to inhibit the growth of 96.3 % of the 27 tested bacteria. Compounds 2-6 displayed selective activities, their inhibitory effects being obtained on 8.3 %, 41.7 %, 58.3 %, 58.3 % and 66.7 % of tested bacteria respectively for 2, 3, 5, 6 and 4. The lowest MIC value of 8 μg/mL was obtained with 6 against Escherichia coli ATCC8739, Enterobacter cloacae ECCI69, Klebsiella pneumoniae KP55, Providencia stuartii NAE16 and Pseudomonas aeruginosa PA01.

Conclusion: The present study demonstrates that Erythrina sigmoidea is a potential source of antibacterial drugs to fight against MDR bacteria. Neobavaisoflavone (6) is the main antibacterial consituents of the bark crude extract.

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Related in: MedlinePlus

Chemical structures of the compounds isolated from Erythrina sigmoidea. sigmoidin I (1); atalantoflavone (2); bidwillon A (3); neocyclomorusin (4); 6α-hydroxyphaseollidin (5); neobavaisoflavone (6)
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Fig1: Chemical structures of the compounds isolated from Erythrina sigmoidea. sigmoidin I (1); atalantoflavone (2); bidwillon A (3); neocyclomorusin (4); 6α-hydroxyphaseollidin (5); neobavaisoflavone (6)

Mentions: Compounds isolated from the bark of Erythrina sigmoidea included β- sigmoidin I (1), atalantoflavone (2), bidwillon A (3), neocyclomorusin (4), 6α-hydroxyphaseollidin (5) and neobavaisoflavone (6) (Fig. 1). Their isolation and identification were previously reported [15]. Chloramphenicol ≥ 98 % (Sigma-Aldrich, St. Quentin Fallavier, France) was used as reference antibiotics (RA) against Gram-negative bacteria. p-Iodonitrotetrazolium chloride ≥ 97 % (INT, Sigma-Aldrich) was used as microbial growth indicator [16, 17].Fig. 1


Antibacterial activities of the methanol extracts and compounds from Erythrina sigmoidea against Gram-negative multi-drug resistant phenotypes.

Djeussi DE, Sandjo LP, Noumedem JA, Omosa LK, T Ngadjui B, Kuete V - BMC Complement Altern Med (2015)

Chemical structures of the compounds isolated from Erythrina sigmoidea. sigmoidin I (1); atalantoflavone (2); bidwillon A (3); neocyclomorusin (4); 6α-hydroxyphaseollidin (5); neobavaisoflavone (6)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4696315&req=5

Fig1: Chemical structures of the compounds isolated from Erythrina sigmoidea. sigmoidin I (1); atalantoflavone (2); bidwillon A (3); neocyclomorusin (4); 6α-hydroxyphaseollidin (5); neobavaisoflavone (6)
Mentions: Compounds isolated from the bark of Erythrina sigmoidea included β- sigmoidin I (1), atalantoflavone (2), bidwillon A (3), neocyclomorusin (4), 6α-hydroxyphaseollidin (5) and neobavaisoflavone (6) (Fig. 1). Their isolation and identification were previously reported [15]. Chloramphenicol ≥ 98 % (Sigma-Aldrich, St. Quentin Fallavier, France) was used as reference antibiotics (RA) against Gram-negative bacteria. p-Iodonitrotetrazolium chloride ≥ 97 % (INT, Sigma-Aldrich) was used as microbial growth indicator [16, 17].Fig. 1

Bottom Line: The MIC results indicated that the crude extracts from the leaves and bark of this plant were able to inhibit the growth of 96.3 % of the 27 tested bacteria.Compounds 2-6 displayed selective activities, their inhibitory effects being obtained on 8.3 %, 41.7 %, 58.3 %, 58.3 % and 66.7 % of tested bacteria respectively for 2, 3, 5, 6 and 4.Neobavaisoflavone (6) is the main antibacterial consituents of the bark crude extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT

Background: In the present study, the methanol extracts from the leaves, as well as compounds namely sigmoidin I (1), atalantoflavone (2), bidwillon A (3), neocyclomorusin (4), 6α-hydroxyphaseollidin (5) and neobavaisoflavone (6) (from the bark extract) were tested for their activities against a panel of Gram-negative bacteria including multi-drug resistant (MDR) phenotypes.

Methods: Broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the extracts as well as compounds 1-6.

Results: The MIC results indicated that the crude extracts from the leaves and bark of this plant were able to inhibit the growth of 96.3 % of the 27 tested bacteria. Compounds 2-6 displayed selective activities, their inhibitory effects being obtained on 8.3 %, 41.7 %, 58.3 %, 58.3 % and 66.7 % of tested bacteria respectively for 2, 3, 5, 6 and 4. The lowest MIC value of 8 μg/mL was obtained with 6 against Escherichia coli ATCC8739, Enterobacter cloacae ECCI69, Klebsiella pneumoniae KP55, Providencia stuartii NAE16 and Pseudomonas aeruginosa PA01.

Conclusion: The present study demonstrates that Erythrina sigmoidea is a potential source of antibacterial drugs to fight against MDR bacteria. Neobavaisoflavone (6) is the main antibacterial consituents of the bark crude extract.

Show MeSH
Related in: MedlinePlus