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A simple novel device for air sampling by electrokinetic capture.

Gordon J, Gandhi P, Shekhawat G, Frazier A, Hampton-Marcell J, Gilbert JA - Microbiome (2015)

Bottom Line: We analyzed particle sizes captured from air, without regard to specific analyte by atomic force microscopy: particles at least as low as 100 nM could be captured from ambient air.This work introduces a very simple plug-and-play device that can sample air at a high-volume flow rate with no moving parts and collect particles down to the sub-micron range.The performance of the device is substantially equivalent to capture by pumping through a filter for microbiome analysis by quantitative PCR and amplicon sequencing.

View Article: PubMed Central - PubMed

Affiliation: Inspirotec LLC, 3307 Meadow Lane, Glenview, IL, 60025, USA. jgordon@inspirotec.com.

ABSTRACT

Background: A variety of different sampling devices are currently available to acquire air samples for the study of the microbiome of the air. All have a degree of technical complexity that limits deployment. Here, we evaluate the use of a novel device, which has no technical complexity and is easily deployable.

Results: An air-cleaning device powered by electrokinetic propulsion has been adapted to provide a universal method for collecting samples of the aerobiome. Plasma-induced charge in aerosol particles causes propulsion to and capture on a counter-electrode. The flow of ions creates net bulk airflow, with no moving parts. A device and electrode assembly have been re-designed from air-cleaning technology to provide an average air flow of 120 lpm. This compares favorably with current air sampling devices based on physical air pumping. Capture efficiency was determined by comparison with a 0.4 μm polycarbonate reference filter, using fluorescent latex particles in a controlled environment chamber. Performance was compared with the same reference filter method in field studies in three different environments. For 23 common fungal species by quantitative polymerase chain reaction (qPCR), there was 100 % sensitivity and apparent specificity of 87 %, with the reference filter taken as "gold standard." Further, bacterial analysis of 16S RNA by amplicon sequencing showed equivalent community structure captured by the electrokinetic device and the reference filter. Unlike other current air sampling methods, capture of particles is determined by charge and so is not controlled by particle mass. We analyzed particle sizes captured from air, without regard to specific analyte by atomic force microscopy: particles at least as low as 100 nM could be captured from ambient air.

Conclusions: This work introduces a very simple plug-and-play device that can sample air at a high-volume flow rate with no moving parts and collect particles down to the sub-micron range. The performance of the device is substantially equivalent to capture by pumping through a filter for microbiome analysis by quantitative PCR and amplicon sequencing.

No MeSH data available.


Related in: MedlinePlus

Procrustes analysis of bacterial community structure as a function of sampling time. A principal coordinate analysis (PCoA) was generated for each of the replicate electrodes and the filter samples. CV1 and CV2 are the first two dimensions of variance that describe the most variance in the multidimensional structure of this population, represented by the percentages on the axes. UniFrac distances from each PCoA were comparatively superimposed by collection time
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Fig3: Procrustes analysis of bacterial community structure as a function of sampling time. A principal coordinate analysis (PCoA) was generated for each of the replicate electrodes and the filter samples. CV1 and CV2 are the first two dimensions of variance that describe the most variance in the multidimensional structure of this population, represented by the percentages on the axes. UniFrac distances from each PCoA were comparatively superimposed by collection time

Mentions: Interestingly, 180 min produced a signal highly similar to the time zero (blank) suggesting that this time frame was insufficient to generate enough biomass for the detection threshold of the amplicon sequencing technology (Fig. 2). However, by 360 min, the community profiles were significantly different from time zero. Reagent-based contamination is known to be an issue [38] and explains the detected signal for blank and 180 min. The significance of the similarity between microbial profiles generated by the filter and Inspirotec Sampler technologies at each time point was assessed using Procrustes analysis including the left and right electrode (technical replicates) of the Inspirotec Sampler as well as the pump-driven filter. Over the course of time, there was no significant difference between either technical replicate or the air filter, despite greater variability between samplers at the starting zero time (Monte Carlo, p > 0.05) (Fig. 3).Fig 3


A simple novel device for air sampling by electrokinetic capture.

Gordon J, Gandhi P, Shekhawat G, Frazier A, Hampton-Marcell J, Gilbert JA - Microbiome (2015)

Procrustes analysis of bacterial community structure as a function of sampling time. A principal coordinate analysis (PCoA) was generated for each of the replicate electrodes and the filter samples. CV1 and CV2 are the first two dimensions of variance that describe the most variance in the multidimensional structure of this population, represented by the percentages on the axes. UniFrac distances from each PCoA were comparatively superimposed by collection time
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4696304&req=5

Fig3: Procrustes analysis of bacterial community structure as a function of sampling time. A principal coordinate analysis (PCoA) was generated for each of the replicate electrodes and the filter samples. CV1 and CV2 are the first two dimensions of variance that describe the most variance in the multidimensional structure of this population, represented by the percentages on the axes. UniFrac distances from each PCoA were comparatively superimposed by collection time
Mentions: Interestingly, 180 min produced a signal highly similar to the time zero (blank) suggesting that this time frame was insufficient to generate enough biomass for the detection threshold of the amplicon sequencing technology (Fig. 2). However, by 360 min, the community profiles were significantly different from time zero. Reagent-based contamination is known to be an issue [38] and explains the detected signal for blank and 180 min. The significance of the similarity between microbial profiles generated by the filter and Inspirotec Sampler technologies at each time point was assessed using Procrustes analysis including the left and right electrode (technical replicates) of the Inspirotec Sampler as well as the pump-driven filter. Over the course of time, there was no significant difference between either technical replicate or the air filter, despite greater variability between samplers at the starting zero time (Monte Carlo, p > 0.05) (Fig. 3).Fig 3

Bottom Line: We analyzed particle sizes captured from air, without regard to specific analyte by atomic force microscopy: particles at least as low as 100 nM could be captured from ambient air.This work introduces a very simple plug-and-play device that can sample air at a high-volume flow rate with no moving parts and collect particles down to the sub-micron range.The performance of the device is substantially equivalent to capture by pumping through a filter for microbiome analysis by quantitative PCR and amplicon sequencing.

View Article: PubMed Central - PubMed

Affiliation: Inspirotec LLC, 3307 Meadow Lane, Glenview, IL, 60025, USA. jgordon@inspirotec.com.

ABSTRACT

Background: A variety of different sampling devices are currently available to acquire air samples for the study of the microbiome of the air. All have a degree of technical complexity that limits deployment. Here, we evaluate the use of a novel device, which has no technical complexity and is easily deployable.

Results: An air-cleaning device powered by electrokinetic propulsion has been adapted to provide a universal method for collecting samples of the aerobiome. Plasma-induced charge in aerosol particles causes propulsion to and capture on a counter-electrode. The flow of ions creates net bulk airflow, with no moving parts. A device and electrode assembly have been re-designed from air-cleaning technology to provide an average air flow of 120 lpm. This compares favorably with current air sampling devices based on physical air pumping. Capture efficiency was determined by comparison with a 0.4 μm polycarbonate reference filter, using fluorescent latex particles in a controlled environment chamber. Performance was compared with the same reference filter method in field studies in three different environments. For 23 common fungal species by quantitative polymerase chain reaction (qPCR), there was 100 % sensitivity and apparent specificity of 87 %, with the reference filter taken as "gold standard." Further, bacterial analysis of 16S RNA by amplicon sequencing showed equivalent community structure captured by the electrokinetic device and the reference filter. Unlike other current air sampling methods, capture of particles is determined by charge and so is not controlled by particle mass. We analyzed particle sizes captured from air, without regard to specific analyte by atomic force microscopy: particles at least as low as 100 nM could be captured from ambient air.

Conclusions: This work introduces a very simple plug-and-play device that can sample air at a high-volume flow rate with no moving parts and collect particles down to the sub-micron range. The performance of the device is substantially equivalent to capture by pumping through a filter for microbiome analysis by quantitative PCR and amplicon sequencing.

No MeSH data available.


Related in: MedlinePlus