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Relationship between glycemic control and histochemical myeloperoxidase activity in neutrophils in patients with type 2 diabetes.

Unubol M, Yavasoglu I, Kacar F, Guney E, Omurlu IK, Ture M, Kadikoylu G, Bolaman Z - Diabetol Metab Syndr (2015)

Bottom Line: Myeloperoxidase plays an important role in oxygen-dependent killing of bacteria, fungi, virus and malignant cells.Myeloperoxidase dye was used for staining.SPSS 16.0 version was used for statistical analyses.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Faculty of Medicine, Adnan Menderes University, 09100 Aydın, Turkey.

ABSTRACT

Background: Myeloperoxidase (MPO) is a lysosomal hemoprotein found in the azurophilic granules in neutrophils. Myeloperoxidase plays an important role in oxygen-dependent killing of bacteria, fungi, virus and malignant cells. Diabetes mellitus (DM) is listed among conditions that may lead to secondary MPO deficiency in neutrophils but inconsistent results concerning MPO activity in diabetic patients have been reported in the literature. In this study, we aimed to evaluate the relationship between glycemic control in patients with type 2 DM and MPO activity in neutrophils from a histochemical perspective.

Methods: The study included 40 patients with type 2 DM with poor glycemic control, 30 patients with type 2 DM with good glycemic control and 31 healthy controls. Peripheral blood smears were analyzed for each patient included in the study. Myeloperoxidase dye was used for staining. Myeloperoxidase ratios in neutrophil were evaluated for proportions of staining with MPO in 100 neutrophils in each smear. SPSS 16.0 version was used for statistical analyses.

Results: Myeloperoxidase ratios in neutrophils were 70 (58.5-80) in type 2 DM patients with poor glycemic control compared to 80 (73.75-90) in those with good glycemic control and 88 (78-92) in healthy controls. The DM group with poor glycemic control was statistically significantly different from the other groups (p < 0.001).

Conclusions: Poor glycemic control in diabetic patients results in decreased MPO activity in neutrophils histochemically.

No MeSH data available.


Related in: MedlinePlus

Correlation between HbA1c and MPO in all groups
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Fig3: Correlation between HbA1c and MPO in all groups

Mentions: Type 2 DM patients with poor glycemic control had a mean age of 56.4 ± 9.99 years and type 2 DM patients with good glycemic control had a mean age of 57.3 ± 10.02 years and healthy controls had a mean age of 51.42 ± 11.48 years, with no significant difference among the groups (p > 0.05). Type 2 DM patients with poor glycemic control had a disease duration of 12.3 ± 3.4 years and were not significantly different from type 2 DM patients with good glycemic control (p > 0.05). Myeloperoxidase ratio in neutrophils was 70 (58.5–80) in type 2 DM patients with poor glycemic control compared to 80 (73.75–90) in those with good glycemic control and 88 (78–92) in healthy controls. The DM group with poor glycemic control was statistically significantly different from the other groups (p < 0.001). Good and poor controlled diabetes groups were similar in terms of the drugs being used. No correlation was found between the MPO activity and the drugs used to treat diabetes (p > 0.05). In patient groups with diabetes, statistically significant differences in terms of MPO activity was not detected among the patients using and not using sitagliptin, insulin detemir, insulin glargine, insulin aspart, insulin lispro, insulin glulisine, metformin, gliclazide, repaglinide, nateglinide, pioglitazone. Statistical results of quantitative and qualitative data are provided in Tables 1, 2, 3 and 4; Figs. 2 and 3 demonstrates correlation analysis between MPO and HbA1c.Table 1


Relationship between glycemic control and histochemical myeloperoxidase activity in neutrophils in patients with type 2 diabetes.

Unubol M, Yavasoglu I, Kacar F, Guney E, Omurlu IK, Ture M, Kadikoylu G, Bolaman Z - Diabetol Metab Syndr (2015)

Correlation between HbA1c and MPO in all groups
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4696277&req=5

Fig3: Correlation between HbA1c and MPO in all groups
Mentions: Type 2 DM patients with poor glycemic control had a mean age of 56.4 ± 9.99 years and type 2 DM patients with good glycemic control had a mean age of 57.3 ± 10.02 years and healthy controls had a mean age of 51.42 ± 11.48 years, with no significant difference among the groups (p > 0.05). Type 2 DM patients with poor glycemic control had a disease duration of 12.3 ± 3.4 years and were not significantly different from type 2 DM patients with good glycemic control (p > 0.05). Myeloperoxidase ratio in neutrophils was 70 (58.5–80) in type 2 DM patients with poor glycemic control compared to 80 (73.75–90) in those with good glycemic control and 88 (78–92) in healthy controls. The DM group with poor glycemic control was statistically significantly different from the other groups (p < 0.001). Good and poor controlled diabetes groups were similar in terms of the drugs being used. No correlation was found between the MPO activity and the drugs used to treat diabetes (p > 0.05). In patient groups with diabetes, statistically significant differences in terms of MPO activity was not detected among the patients using and not using sitagliptin, insulin detemir, insulin glargine, insulin aspart, insulin lispro, insulin glulisine, metformin, gliclazide, repaglinide, nateglinide, pioglitazone. Statistical results of quantitative and qualitative data are provided in Tables 1, 2, 3 and 4; Figs. 2 and 3 demonstrates correlation analysis between MPO and HbA1c.Table 1

Bottom Line: Myeloperoxidase plays an important role in oxygen-dependent killing of bacteria, fungi, virus and malignant cells.Myeloperoxidase dye was used for staining.SPSS 16.0 version was used for statistical analyses.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Faculty of Medicine, Adnan Menderes University, 09100 Aydın, Turkey.

ABSTRACT

Background: Myeloperoxidase (MPO) is a lysosomal hemoprotein found in the azurophilic granules in neutrophils. Myeloperoxidase plays an important role in oxygen-dependent killing of bacteria, fungi, virus and malignant cells. Diabetes mellitus (DM) is listed among conditions that may lead to secondary MPO deficiency in neutrophils but inconsistent results concerning MPO activity in diabetic patients have been reported in the literature. In this study, we aimed to evaluate the relationship between glycemic control in patients with type 2 DM and MPO activity in neutrophils from a histochemical perspective.

Methods: The study included 40 patients with type 2 DM with poor glycemic control, 30 patients with type 2 DM with good glycemic control and 31 healthy controls. Peripheral blood smears were analyzed for each patient included in the study. Myeloperoxidase dye was used for staining. Myeloperoxidase ratios in neutrophil were evaluated for proportions of staining with MPO in 100 neutrophils in each smear. SPSS 16.0 version was used for statistical analyses.

Results: Myeloperoxidase ratios in neutrophils were 70 (58.5-80) in type 2 DM patients with poor glycemic control compared to 80 (73.75-90) in those with good glycemic control and 88 (78-92) in healthy controls. The DM group with poor glycemic control was statistically significantly different from the other groups (p < 0.001).

Conclusions: Poor glycemic control in diabetic patients results in decreased MPO activity in neutrophils histochemically.

No MeSH data available.


Related in: MedlinePlus