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Rapid transesterification of micro-amount of lipids from microalgae via a micro-mixer reactor.

Liu J, Chu Y, Cao X, Zhao Y, Xie H, Xue S - Biotechnol Biofuels (2015)

Bottom Line: The minimum amount of sample was decreased to 30 µg lipids.The new approach was successfully applied to the fatty acid composition analysis of soybean oil and microalgal lipids.The micro-mixer reactor has great potential for applications not only in large-scale biodiesel production but also for the micro-scale analysis of microalgae fatty acid compositions.

View Article: PubMed Central - PubMed

Affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 16023 China ; University of Chinese Academy of Sciences, Beijing, 100049 China.

ABSTRACT

Background: Fatty acid composition is an important physiological parameter of microalgae, which is taken as the third generation alternative resource of biodiesel. To boost microalgal research and applications, a convenient, rapid, and acid-catalyzed transesterification procedure that satisfies the demand for the analysis of the fatty acid composition of lipids with micro-scale samples in the high-throughput screening of microalgal strains is needed, along with the evaluation of the physiological status of microalgae in response to nutrient stress.

Results: The reaction conditions of transesterification via a micro-mixer reactor were optimized as follows: 90 °C reaction temperature, 20 min reaction time, 6:1 volume ratio of H2SO4-methanol to lipid-in-hexane, and a Y-type micro-mixer with a 20-m-long extended loop that has a 0.3 mm diameter. The minimum amount of sample was decreased to 30 µg lipids. The new approach was successfully applied to the fatty acid composition analysis of soybean oil and microalgal lipids. Definitely, it could be applied to acyl related oils from different sources.

Conclusion: Here, we have developed a simple and rapid method for the analysis of the fatty acid composition of lipids. The new method requires less than 20 min for transesterification and a minimum of only 30 µg lipid sample. Furthermore, a high-throughput process can be easily realized by numbering up the micro-mixer reactors. The micro-mixer reactor has great potential for applications not only in large-scale biodiesel production but also for the micro-scale analysis of microalgae fatty acid compositions.

No MeSH data available.


Related in: MedlinePlus

FAME yields obtained in three types of MRs at different reaction temperatures
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Fig2: FAME yields obtained in three types of MRs at different reaction temperatures

Mentions: In a MR, the mixing efficiency largely depends on geometric structures [12, 17]; temperature is also a very important reaction factor for transesterification. Here, three types of MRs with simple structures, as shown in Fig. 1, were used at different reaction temperatures to perform the transesterification of glyceryl trioleate (TAG). The diameter of the extended loop capillary was 0.3 mm, and the reaction time was 20 min at a 6:1 volume ratio of H2SO4-methanol to  lipid-in-hexane. The FAME yields obtained by transesterification in the three types of MRs are shown in Fig. 2.Fig. 1


Rapid transesterification of micro-amount of lipids from microalgae via a micro-mixer reactor.

Liu J, Chu Y, Cao X, Zhao Y, Xie H, Xue S - Biotechnol Biofuels (2015)

FAME yields obtained in three types of MRs at different reaction temperatures
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4696256&req=5

Fig2: FAME yields obtained in three types of MRs at different reaction temperatures
Mentions: In a MR, the mixing efficiency largely depends on geometric structures [12, 17]; temperature is also a very important reaction factor for transesterification. Here, three types of MRs with simple structures, as shown in Fig. 1, were used at different reaction temperatures to perform the transesterification of glyceryl trioleate (TAG). The diameter of the extended loop capillary was 0.3 mm, and the reaction time was 20 min at a 6:1 volume ratio of H2SO4-methanol to  lipid-in-hexane. The FAME yields obtained by transesterification in the three types of MRs are shown in Fig. 2.Fig. 1

Bottom Line: The minimum amount of sample was decreased to 30 µg lipids.The new approach was successfully applied to the fatty acid composition analysis of soybean oil and microalgal lipids.The micro-mixer reactor has great potential for applications not only in large-scale biodiesel production but also for the micro-scale analysis of microalgae fatty acid compositions.

View Article: PubMed Central - PubMed

Affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 16023 China ; University of Chinese Academy of Sciences, Beijing, 100049 China.

ABSTRACT

Background: Fatty acid composition is an important physiological parameter of microalgae, which is taken as the third generation alternative resource of biodiesel. To boost microalgal research and applications, a convenient, rapid, and acid-catalyzed transesterification procedure that satisfies the demand for the analysis of the fatty acid composition of lipids with micro-scale samples in the high-throughput screening of microalgal strains is needed, along with the evaluation of the physiological status of microalgae in response to nutrient stress.

Results: The reaction conditions of transesterification via a micro-mixer reactor were optimized as follows: 90 °C reaction temperature, 20 min reaction time, 6:1 volume ratio of H2SO4-methanol to lipid-in-hexane, and a Y-type micro-mixer with a 20-m-long extended loop that has a 0.3 mm diameter. The minimum amount of sample was decreased to 30 µg lipids. The new approach was successfully applied to the fatty acid composition analysis of soybean oil and microalgal lipids. Definitely, it could be applied to acyl related oils from different sources.

Conclusion: Here, we have developed a simple and rapid method for the analysis of the fatty acid composition of lipids. The new method requires less than 20 min for transesterification and a minimum of only 30 µg lipid sample. Furthermore, a high-throughput process can be easily realized by numbering up the micro-mixer reactors. The micro-mixer reactor has great potential for applications not only in large-scale biodiesel production but also for the micro-scale analysis of microalgae fatty acid compositions.

No MeSH data available.


Related in: MedlinePlus