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Protocol for assessing maternal, environmental and epigenetic risk factors for dental caries in children.

Fernando S, Speicher DJ, Bakr MM, Benton MC, Lea RA, Scuffham PA, Mihala G, Johnson NW - BMC Oral Health (2015)

Bottom Line: Subjects' height, weight and mid-waist circumference are taken and Body Mass Index (BMI) computed, using an electronic Bio-Impedance balance.Salivary Deoxyribose Nucleic Acid (DNA) is extracted for genetic studies including epigenetics using the SeqCap Epi Enrichment Kit.The findings will provide an evidence base to plan and implement improved preventive strategies.

View Article: PubMed Central - PubMed

Affiliation: School of Dentistry and Oral Health, Gold Coast Campus, Griffith University, Gold Coast, 4222, Queensland, Australia. Surani.fernando@griffith.edu.au.

ABSTRACT

Background: Expenditure on dental and oral health services in Australia is $3.4 billion AUD annually. This is the sixth highest health cost and accounts for 7 % of total national health expenditure. Approximately 49 % of Australian children aged 6 years have caries experience in their deciduous teeth and this is rising. The aetiology of dental caries involves a complex interplay of individual, behavioural, social, economic, political and environmental conditions, and there is increasing interest in genetic predisposition and epigenetic modification.

Methods: The Oral Health Sub-study; a cross sectional study of a birth cohort began in November 2012 by examining mothers and their children who were six years old by the time of initiation of the study, which is ongoing. Data from detailed questionnaires of families from birth onwards and data on mothers' knowledge, attitudes and practices towards oral health collected at the time of clinical examination are used. Subjects' height, weight and mid-waist circumference are taken and Body Mass Index (BMI) computed, using an electronic Bio-Impedance balance. Dental caries experience is scored using the International Caries Detection and Assessment System (ICDAS). Saliva is collected for physiological measures. Salivary Deoxyribose Nucleic Acid (DNA) is extracted for genetic studies including epigenetics using the SeqCap Epi Enrichment Kit. Targets of interest are being confirmed by pyrosequencing to identify potential epigenetic markers of caries risk.

Discussion: This study will examine a wide range of potential determinants for childhood dental caries and evaluate inter-relationships amongst them. The findings will provide an evidence base to plan and implement improved preventive strategies.

No MeSH data available.


Related in: MedlinePlus

Flow chart showing the process of data collection
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Related In: Results  -  Collection

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Fig2: Flow chart showing the process of data collection

Mentions: In 2012, mothers of the first cohort of children, who were 6 years old at the time, were sent letters alerting them to the oral health study, followed by a telephone call to assess their level of interest. Since then we went on to recruit participants from 2007 to 2009 cohorts and the recruitments are still taking place. Participants who find it difficult to travel far, who have serious illness and who are not willing to undergo a detailed oral examination are excluded from the study. Those who agree to attend the dental clinic at Griffith Dental School are given appointments for a free dental examination of the mother and child by two qualified dental surgeons, which includes offer of free treatment for the child, if required. Both mother and child are asked not to brush their teeth 2–3 h prior to examination, not to consume sweet food or beverages, smoke or use a mouth wash as per oral examination criteria. Mothers who are pregnant or who are wearing cardiac pace makers are excluded from weighing on the bio impedance scale. Detailed medical histories are taken when subjects arrive at chair side (Fig. 2). A careful examination of the head and neck, visually and by palpation, is performed. Each participant is then examined for salivary physiology, for the health of the oral soft tissues, for dental status and for experience of dental caries and periodontal disease (Fig. 2). Data are entered into the Titanium clinical management software (Spark Dental Technology, New Zealand) of the Griffith University Dental Clinic, incorporating pages specifically designed for the recording of research observations. Participants who are in need of treatment are referred for treatment at Griffith University dental clinics, that for children being free, the costs being covered by the Queensland Government.Fig. 2


Protocol for assessing maternal, environmental and epigenetic risk factors for dental caries in children.

Fernando S, Speicher DJ, Bakr MM, Benton MC, Lea RA, Scuffham PA, Mihala G, Johnson NW - BMC Oral Health (2015)

Flow chart showing the process of data collection
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4696221&req=5

Fig2: Flow chart showing the process of data collection
Mentions: In 2012, mothers of the first cohort of children, who were 6 years old at the time, were sent letters alerting them to the oral health study, followed by a telephone call to assess their level of interest. Since then we went on to recruit participants from 2007 to 2009 cohorts and the recruitments are still taking place. Participants who find it difficult to travel far, who have serious illness and who are not willing to undergo a detailed oral examination are excluded from the study. Those who agree to attend the dental clinic at Griffith Dental School are given appointments for a free dental examination of the mother and child by two qualified dental surgeons, which includes offer of free treatment for the child, if required. Both mother and child are asked not to brush their teeth 2–3 h prior to examination, not to consume sweet food or beverages, smoke or use a mouth wash as per oral examination criteria. Mothers who are pregnant or who are wearing cardiac pace makers are excluded from weighing on the bio impedance scale. Detailed medical histories are taken when subjects arrive at chair side (Fig. 2). A careful examination of the head and neck, visually and by palpation, is performed. Each participant is then examined for salivary physiology, for the health of the oral soft tissues, for dental status and for experience of dental caries and periodontal disease (Fig. 2). Data are entered into the Titanium clinical management software (Spark Dental Technology, New Zealand) of the Griffith University Dental Clinic, incorporating pages specifically designed for the recording of research observations. Participants who are in need of treatment are referred for treatment at Griffith University dental clinics, that for children being free, the costs being covered by the Queensland Government.Fig. 2

Bottom Line: Subjects' height, weight and mid-waist circumference are taken and Body Mass Index (BMI) computed, using an electronic Bio-Impedance balance.Salivary Deoxyribose Nucleic Acid (DNA) is extracted for genetic studies including epigenetics using the SeqCap Epi Enrichment Kit.The findings will provide an evidence base to plan and implement improved preventive strategies.

View Article: PubMed Central - PubMed

Affiliation: School of Dentistry and Oral Health, Gold Coast Campus, Griffith University, Gold Coast, 4222, Queensland, Australia. Surani.fernando@griffith.edu.au.

ABSTRACT

Background: Expenditure on dental and oral health services in Australia is $3.4 billion AUD annually. This is the sixth highest health cost and accounts for 7 % of total national health expenditure. Approximately 49 % of Australian children aged 6 years have caries experience in their deciduous teeth and this is rising. The aetiology of dental caries involves a complex interplay of individual, behavioural, social, economic, political and environmental conditions, and there is increasing interest in genetic predisposition and epigenetic modification.

Methods: The Oral Health Sub-study; a cross sectional study of a birth cohort began in November 2012 by examining mothers and their children who were six years old by the time of initiation of the study, which is ongoing. Data from detailed questionnaires of families from birth onwards and data on mothers' knowledge, attitudes and practices towards oral health collected at the time of clinical examination are used. Subjects' height, weight and mid-waist circumference are taken and Body Mass Index (BMI) computed, using an electronic Bio-Impedance balance. Dental caries experience is scored using the International Caries Detection and Assessment System (ICDAS). Saliva is collected for physiological measures. Salivary Deoxyribose Nucleic Acid (DNA) is extracted for genetic studies including epigenetics using the SeqCap Epi Enrichment Kit. Targets of interest are being confirmed by pyrosequencing to identify potential epigenetic markers of caries risk.

Discussion: This study will examine a wide range of potential determinants for childhood dental caries and evaluate inter-relationships amongst them. The findings will provide an evidence base to plan and implement improved preventive strategies.

No MeSH data available.


Related in: MedlinePlus