Algorithmic co-optimization of genetic constructs and growth conditions: application to 6-ACA, a potential nylon-6 precursor.
Bottom Line: This is compared to a 64-member full factorial library just varying expression (0.64 Mb of DNA assembly).Statistical analysis of the screening data from these libraries leads to different predictions as to whether the expression of enzymes needs to increase or decrease.This work introduces a generalizable platform to co-optimize genetic and non-genetic factors.
Affiliation: Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.Show MeSH
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Mentions: We selected a de novo pathway for 6-aminocaproic acid (6-ACA) biosynthesis in Escherichia coli (E. coli) for proof-of-principle experiments (unpublished results) (Figure 1A). 6-ACA is the linear form of caprolactam, which is the chemical building block of nylon-6. Nylon is the most highly produced synthetic fiber globally (about 4M tons/yr). The fossil-based chemical process for producing caprolactam leads to significant greenhouse gas emission, quantified by its global warming potential (GWP). Depending on the origin of the carbon and energy sources used, the GWP for bio-based production has the potential to be 91% lower than that of the chemical caprolactam route, which is considered to be a sustainable and green process (unpublished results).
Affiliation: Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.