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Injectable gellan gum-based nanoparticles-loaded system for the local delivery of vancomycin in osteomyelitis treatment.

Posadowska U, Brzychczy-Wloch M, Pamula E - J Mater Sci Mater Med (2015)

Bottom Line: The resulting system was found to be injectable (extrusion force 11.3 ± 1.1 N), reassembled its structure after breaking as shown by rheology tests and ensured required burst release followed by sustained Vanc delivery.The system was cytocompatible with osteoblast-like MG-63 cells (no significant impact on cells' viability was detected).As a result we obtained a biocompatible system displaying ease of application (low extrusion force), self-healing ability after disruption, adjustable drug release and antimicrobial properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059, Krakow, Poland. uposadow@agh.edu.pl.

ABSTRACT
Infection spreading in the skeletal system leading to osteomyelitis can be prevented by the prolonged administration of antibiotics in high doses. However systemic antibiotherapy, besides its inconvenience and often low efficacy, provokes numerous side effects. Thus, we formulated a new injectable nanoparticle-loaded system for the local delivery of vancomycin (Vanc) applied in a minimally-invasive way. Vanc was encapsulated in poly(L-lactide-co-glycolide) nanoparticles (NPs) by double-emulsification. The size (258 ± 11 nm), polydispersity index (0.240 ± 0.003) and surface potential (-25.9 ± 0.2 mV) of NPs were determined by dynamic light scattering and capillary electrophoresis measurements. They have a spherical morphology and a smooth topography as observed using atomic force microscopy. Vanc loading and encapsulation efficiencies were 8.8 ± 0.1 and 55.2 ± 0.5 %, respectively, based on fluorescence spectroscopy assays. In order to ensure injectability, NPs were suspended in gellan gum and cross-linked with Ca(2+); also a portion of dissolved antibiotic was added to the system. The resulting system was found to be injectable (extrusion force 11.3 ± 1.1 N), reassembled its structure after breaking as shown by rheology tests and ensured required burst release followed by sustained Vanc delivery. The system was cytocompatible with osteoblast-like MG-63 cells (no significant impact on cells' viability was detected). Growth of Staphylococcus spp. reference strains and also those isolated from osteomyelitic joints was inhibited in contact with the injectable system. As a result we obtained a biocompatible system displaying ease of application (low extrusion force), self-healing ability after disruption, adjustable drug release and antimicrobial properties.

No MeSH data available.


Related in: MedlinePlus

Inhibition of Staphylococcus spp. (DSM 24167—reference strain of S.aureus, SA1-KCR—isolate of S.aureus from infected joint, ATCC 700296—reference strain of S. epidermidis, SE1-KCR—isolate of S. epidermidis from infected joint) grown with GG-Vanc-NPs. Microphotographs were taken on Petri dish (diameter 9 cm), with a digital camera Olympus; the samples were introduced inside the 3 mm agar well or placed directly on the agar, for the left and right area of the Petri dish, respectively.  Mean ± SEM
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Fig4: Inhibition of Staphylococcus spp. (DSM 24167—reference strain of S.aureus, SA1-KCR—isolate of S.aureus from infected joint, ATCC 700296—reference strain of S. epidermidis, SE1-KCR—isolate of S. epidermidis from infected joint) grown with GG-Vanc-NPs. Microphotographs were taken on Petri dish (diameter 9 cm), with a digital camera Olympus; the samples were introduced inside the 3 mm agar well or placed directly on the agar, for the left and right area of the Petri dish, respectively.  Mean ± SEM

Mentions: In Fig. 4 inhibition zones and microphotographs of agar plates with Staphylococcus spp. cultured with GG-Vanc-NPs are presented. The diameters obtained were in the range 24.1–29 mm (depending on the way the samples were placed and type of the strain). For the vancomycin standards (30 µg) the zone diameter was 27–28 mm. For GG sample growth of Staphylococcus spp. was not inhibited (data not shown).


Injectable gellan gum-based nanoparticles-loaded system for the local delivery of vancomycin in osteomyelitis treatment.

Posadowska U, Brzychczy-Wloch M, Pamula E - J Mater Sci Mater Med (2015)

Inhibition of Staphylococcus spp. (DSM 24167—reference strain of S.aureus, SA1-KCR—isolate of S.aureus from infected joint, ATCC 700296—reference strain of S. epidermidis, SE1-KCR—isolate of S. epidermidis from infected joint) grown with GG-Vanc-NPs. Microphotographs were taken on Petri dish (diameter 9 cm), with a digital camera Olympus; the samples were introduced inside the 3 mm agar well or placed directly on the agar, for the left and right area of the Petri dish, respectively.  Mean ± SEM
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4666281&req=5

Fig4: Inhibition of Staphylococcus spp. (DSM 24167—reference strain of S.aureus, SA1-KCR—isolate of S.aureus from infected joint, ATCC 700296—reference strain of S. epidermidis, SE1-KCR—isolate of S. epidermidis from infected joint) grown with GG-Vanc-NPs. Microphotographs were taken on Petri dish (diameter 9 cm), with a digital camera Olympus; the samples were introduced inside the 3 mm agar well or placed directly on the agar, for the left and right area of the Petri dish, respectively.  Mean ± SEM
Mentions: In Fig. 4 inhibition zones and microphotographs of agar plates with Staphylococcus spp. cultured with GG-Vanc-NPs are presented. The diameters obtained were in the range 24.1–29 mm (depending on the way the samples were placed and type of the strain). For the vancomycin standards (30 µg) the zone diameter was 27–28 mm. For GG sample growth of Staphylococcus spp. was not inhibited (data not shown).

Bottom Line: The resulting system was found to be injectable (extrusion force 11.3 ± 1.1 N), reassembled its structure after breaking as shown by rheology tests and ensured required burst release followed by sustained Vanc delivery.The system was cytocompatible with osteoblast-like MG-63 cells (no significant impact on cells' viability was detected).As a result we obtained a biocompatible system displaying ease of application (low extrusion force), self-healing ability after disruption, adjustable drug release and antimicrobial properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, al. A. Mickiewicza 30, 30-059, Krakow, Poland. uposadow@agh.edu.pl.

ABSTRACT
Infection spreading in the skeletal system leading to osteomyelitis can be prevented by the prolonged administration of antibiotics in high doses. However systemic antibiotherapy, besides its inconvenience and often low efficacy, provokes numerous side effects. Thus, we formulated a new injectable nanoparticle-loaded system for the local delivery of vancomycin (Vanc) applied in a minimally-invasive way. Vanc was encapsulated in poly(L-lactide-co-glycolide) nanoparticles (NPs) by double-emulsification. The size (258 ± 11 nm), polydispersity index (0.240 ± 0.003) and surface potential (-25.9 ± 0.2 mV) of NPs were determined by dynamic light scattering and capillary electrophoresis measurements. They have a spherical morphology and a smooth topography as observed using atomic force microscopy. Vanc loading and encapsulation efficiencies were 8.8 ± 0.1 and 55.2 ± 0.5 %, respectively, based on fluorescence spectroscopy assays. In order to ensure injectability, NPs were suspended in gellan gum and cross-linked with Ca(2+); also a portion of dissolved antibiotic was added to the system. The resulting system was found to be injectable (extrusion force 11.3 ± 1.1 N), reassembled its structure after breaking as shown by rheology tests and ensured required burst release followed by sustained Vanc delivery. The system was cytocompatible with osteoblast-like MG-63 cells (no significant impact on cells' viability was detected). Growth of Staphylococcus spp. reference strains and also those isolated from osteomyelitic joints was inhibited in contact with the injectable system. As a result we obtained a biocompatible system displaying ease of application (low extrusion force), self-healing ability after disruption, adjustable drug release and antimicrobial properties.

No MeSH data available.


Related in: MedlinePlus