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Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids.

Yang ZH, Inoue S, Taniguchi Y, Miyahara H, Iwasaki Y, Takeo J, Sakaue H, Nakaya Y - Lipids Health Dis (2015)

Bottom Line: Consumption of EPA reduced plasma lipid levels and hepatic lipid deposition, and decreased the fatty acid desaturation index in liver and adipose tissue.Consumption of LCMUFA decreased plasma non-HDL cholesterol, improved hyperinsulinemia, and decreased the fatty acid desaturation index in adipose tissue.Our results suggest that saury oil-mediated improvement of metabolic syndrome in diet-induced obese mice may possibly be due to a combined effect of n-3 PUFA and LCMUFA.

View Article: PubMed Central - PubMed

Affiliation: Central Research Laboratory, Nippon Suisan Kaisha, 32-3 Nanakuni 1 Chome, Hachioji, Tokyo, 192-0991, Japan. zhihong.yang@nih.gov.

ABSTRACT

Background: Pacific saury is a common dietary component in East Asia. Saury oil contains considerable levels of n-3 unsaturated fatty acids (PUFA) and long-chain monounsaturated fatty acids (LCMUFA) with aliphatic tails longer than 18 carbons. In our previous study, consumption of saury oil for 4 to 6 wk improved insulin sensitivity and the plasma lipid profile in mice. However, the long-term effects of saury oil on metabolic syndrome (MetS) risk factors remain to be demonstrated. In the current study, we examined the long-term effects of saury oil on mice fed a high-fat diet, and compared the effect of n-3 PUFA EPA and LCMUFA on MetS risk factor in diet-induced obese mice.

Methods and results: In Experiment 1, male C57BL/6 J mice were fed either a 32% lard diet (control) or a diet containing 22% lard plus 10% saury oil (saury oil group) for 18 weeks. Although no differences were found in body weight and energy expenditure between the control and saury oil groups, the saury oil diet decreased plasma insulin, non-HDL cholesterol, hepatic steatosis, and adipocyte size, and altered levels of mRNA transcribed from genes involved in insulin signaling and inflammation in adipose tissue. Organ and plasma fatty acid profile analysis revealed that consumption of saury oil increased n-3 PUFA and LCMUFA (especially n-11 LCMUFA) levels in multiple organs, and decreased the fatty acid desaturation index (C16:1/C16:0; C18:1/C18:0) in liver and adipose tissue. In Experiment 2, male C57BL/6 J mice were fed a 32% lard diet (control), a diet containing 28% lard plus 4% EPA (EPA group), or a diet containing 20% lard plus 12% LCMUFA concentrate (LCMUFA group) for 8 weeks. EPA or LCMUFA intake increased organ levels of EPA and LCMUFA, respectively. Consumption of EPA reduced plasma lipid levels and hepatic lipid deposition, and decreased the fatty acid desaturation index in liver and adipose tissue. Consumption of LCMUFA decreased plasma non-HDL cholesterol, improved hyperinsulinemia, and decreased the fatty acid desaturation index in adipose tissue. EPA accumulated mainly in liver, and LCMUFA (especially n-11 LCMUFA) accumulated mainly in white adipose tissue, suggesting their possible individual biological effects for improving MetS.

Conclusion: Our results suggest that saury oil-mediated improvement of metabolic syndrome in diet-induced obese mice may possibly be due to a combined effect of n-3 PUFA and LCMUFA.

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Related in: MedlinePlus

Organ fatty acid compositions in diet-induced obese C57BL/6 J mice fed the control, EPA or LCMUFA diet for 8 weeks. Fatty acid composition in liver (a) and epididymal adipose tissue (b) at the end of 8 weeks. Values are means ± SEM, n = 10. Significantly different mean values (P < 0.05) are indicated by different lowercase letters
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Fig10: Organ fatty acid compositions in diet-induced obese C57BL/6 J mice fed the control, EPA or LCMUFA diet for 8 weeks. Fatty acid composition in liver (a) and epididymal adipose tissue (b) at the end of 8 weeks. Values are means ± SEM, n = 10. Significantly different mean values (P < 0.05) are indicated by different lowercase letters

Mentions: As expected, the fatty acid composition in liver and WAT also reflected the dietary perturbations (Fig. 10). In general, mice fed the EPA diet had proportionally more EPA (~14- to 49-fold) than the control and LCMUFA-fed groups, and proportionally more LCMUFAs (~2- to 9-fold) were found in the LCMUFA group compared with control or EPA groups. For individual types of LCMUFA, there were no significant differences between the control and EPA group. In general, compared with the control or EPA group, each type of LCMUFA had a greater fold change (~1.3 to 205-fold increase) in WAT than in liver (~0.05 to 161-fold increase). Also, as compared with the control or EPA group, the changes in n-11 LCMUFA (C20:1 n-11, ~18 to 161-fold; C22:1 n-11, ~41 to 205-fold increase) were greater than for n-9 LCMUFA (C20:1 n-9, ~0.05 to 1.5-fold; C22:1 n-9, ~0.4 to 12-fold increase) in WAT and liver tissues (Fig. 11). Furthermore, both the EPA and LCMUFA diets decreased the ratios of C16:1/C16:0 (~7–15 %) (P < 0.05) and C18:1/C18:0 (~11–33 %) (P < 0.05) in WAT as compared with the control group, and the EPA diet decreased the ratio of C16:1/C16:0 by 28 % (P < 0.05) and C18:1/C18:0 by 62 % (P < 0.05) in the liver (Fig. 5b).Fig. 10


Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids.

Yang ZH, Inoue S, Taniguchi Y, Miyahara H, Iwasaki Y, Takeo J, Sakaue H, Nakaya Y - Lipids Health Dis (2015)

Organ fatty acid compositions in diet-induced obese C57BL/6 J mice fed the control, EPA or LCMUFA diet for 8 weeks. Fatty acid composition in liver (a) and epididymal adipose tissue (b) at the end of 8 weeks. Values are means ± SEM, n = 10. Significantly different mean values (P < 0.05) are indicated by different lowercase letters
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4666194&req=5

Fig10: Organ fatty acid compositions in diet-induced obese C57BL/6 J mice fed the control, EPA or LCMUFA diet for 8 weeks. Fatty acid composition in liver (a) and epididymal adipose tissue (b) at the end of 8 weeks. Values are means ± SEM, n = 10. Significantly different mean values (P < 0.05) are indicated by different lowercase letters
Mentions: As expected, the fatty acid composition in liver and WAT also reflected the dietary perturbations (Fig. 10). In general, mice fed the EPA diet had proportionally more EPA (~14- to 49-fold) than the control and LCMUFA-fed groups, and proportionally more LCMUFAs (~2- to 9-fold) were found in the LCMUFA group compared with control or EPA groups. For individual types of LCMUFA, there were no significant differences between the control and EPA group. In general, compared with the control or EPA group, each type of LCMUFA had a greater fold change (~1.3 to 205-fold increase) in WAT than in liver (~0.05 to 161-fold increase). Also, as compared with the control or EPA group, the changes in n-11 LCMUFA (C20:1 n-11, ~18 to 161-fold; C22:1 n-11, ~41 to 205-fold increase) were greater than for n-9 LCMUFA (C20:1 n-9, ~0.05 to 1.5-fold; C22:1 n-9, ~0.4 to 12-fold increase) in WAT and liver tissues (Fig. 11). Furthermore, both the EPA and LCMUFA diets decreased the ratios of C16:1/C16:0 (~7–15 %) (P < 0.05) and C18:1/C18:0 (~11–33 %) (P < 0.05) in WAT as compared with the control group, and the EPA diet decreased the ratio of C16:1/C16:0 by 28 % (P < 0.05) and C18:1/C18:0 by 62 % (P < 0.05) in the liver (Fig. 5b).Fig. 10

Bottom Line: Consumption of EPA reduced plasma lipid levels and hepatic lipid deposition, and decreased the fatty acid desaturation index in liver and adipose tissue.Consumption of LCMUFA decreased plasma non-HDL cholesterol, improved hyperinsulinemia, and decreased the fatty acid desaturation index in adipose tissue.Our results suggest that saury oil-mediated improvement of metabolic syndrome in diet-induced obese mice may possibly be due to a combined effect of n-3 PUFA and LCMUFA.

View Article: PubMed Central - PubMed

Affiliation: Central Research Laboratory, Nippon Suisan Kaisha, 32-3 Nanakuni 1 Chome, Hachioji, Tokyo, 192-0991, Japan. zhihong.yang@nih.gov.

ABSTRACT

Background: Pacific saury is a common dietary component in East Asia. Saury oil contains considerable levels of n-3 unsaturated fatty acids (PUFA) and long-chain monounsaturated fatty acids (LCMUFA) with aliphatic tails longer than 18 carbons. In our previous study, consumption of saury oil for 4 to 6 wk improved insulin sensitivity and the plasma lipid profile in mice. However, the long-term effects of saury oil on metabolic syndrome (MetS) risk factors remain to be demonstrated. In the current study, we examined the long-term effects of saury oil on mice fed a high-fat diet, and compared the effect of n-3 PUFA EPA and LCMUFA on MetS risk factor in diet-induced obese mice.

Methods and results: In Experiment 1, male C57BL/6 J mice were fed either a 32% lard diet (control) or a diet containing 22% lard plus 10% saury oil (saury oil group) for 18 weeks. Although no differences were found in body weight and energy expenditure between the control and saury oil groups, the saury oil diet decreased plasma insulin, non-HDL cholesterol, hepatic steatosis, and adipocyte size, and altered levels of mRNA transcribed from genes involved in insulin signaling and inflammation in adipose tissue. Organ and plasma fatty acid profile analysis revealed that consumption of saury oil increased n-3 PUFA and LCMUFA (especially n-11 LCMUFA) levels in multiple organs, and decreased the fatty acid desaturation index (C16:1/C16:0; C18:1/C18:0) in liver and adipose tissue. In Experiment 2, male C57BL/6 J mice were fed a 32% lard diet (control), a diet containing 28% lard plus 4% EPA (EPA group), or a diet containing 20% lard plus 12% LCMUFA concentrate (LCMUFA group) for 8 weeks. EPA or LCMUFA intake increased organ levels of EPA and LCMUFA, respectively. Consumption of EPA reduced plasma lipid levels and hepatic lipid deposition, and decreased the fatty acid desaturation index in liver and adipose tissue. Consumption of LCMUFA decreased plasma non-HDL cholesterol, improved hyperinsulinemia, and decreased the fatty acid desaturation index in adipose tissue. EPA accumulated mainly in liver, and LCMUFA (especially n-11 LCMUFA) accumulated mainly in white adipose tissue, suggesting their possible individual biological effects for improving MetS.

Conclusion: Our results suggest that saury oil-mediated improvement of metabolic syndrome in diet-induced obese mice may possibly be due to a combined effect of n-3 PUFA and LCMUFA.

Show MeSH
Related in: MedlinePlus