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Presence of the knockdown resistance mutation, Vgsc-1014F in Anopheles gambiae and An. arabiensis in western Kenya.

Ochomo E, Subramaniam K, Kemei B, Rippon E, Bayoh NM, Kamau L, Atieli F, Vulule JM, Ouma C, Gimnig J, Donnelly MJ, Mbogo C - Parasit Vectors (2015)

Bottom Line: A portion of the samples were submitted for sequencing in order to confirm the Vgsc-1014F genotyping results.The resulting sequence data were deposited in GenBank (Accession numbers: KR867642-KR867651, KT758295-KT758303).It is important that we start monitoring relative frequencies of the two kdr genes so that we can determine their relative importance in an area of high insecticide treated net ownership.

View Article: PubMed Central - PubMed

Affiliation: School of Public Health and Community Development, Maseno University, Maseno, Kenya. eochomo@kemricdc.org.

ABSTRACT

Introduction: The voltage gated sodium channel mutation Vgsc-1014S (kdr-east) was first reported in Kenya in 2000 and has since been observed to occur at high frequencies in the local Anopheles gambiae s.s.

Population: The mutation Vgsc-1014F has never been reported from An. gambiae Complex complex mosquitoes in Kenya.

Findings: Molecularly confirmed An. gambiae s.s. (hereafter An. gambiae) and An. arabiensis collected from 4 different parts of western Kenya were genotyped for kdr from 2011 to 2013. Vgsc-1014F was observed to have emerged, apparently, simultaneously in both An. gambiae and An. arabiensis in 2012. A portion of the samples were submitted for sequencing in order to confirm the Vgsc-1014F genotyping results. The resulting sequence data were deposited in GenBank (Accession numbers: KR867642-KR867651, KT758295-KT758303). A single Vgsc-1014F haplotype was observed suggesting, a common origin in both species.

Conclusion: This is the first report of Vgsc-1014F in Kenya. Based on our samples, the mutation is present in low frequencies in both An. gambiae and An. arabiensis. It is important that we start monitoring relative frequencies of the two kdr genes so that we can determine their relative importance in an area of high insecticide treated net ownership.

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A TCS plot of the three haplotypes present in the populations assayed. White colour represents An. gambiae while black colour represents An. arabiensis
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Fig1: A TCS plot of the three haplotypes present in the populations assayed. White colour represents An. gambiae while black colour represents An. arabiensis

Mentions: Only a single 1014F haplotype was observed (Fig. 1), suggesting a common origin in the species and subsequent interspecific transfer. However it should be noted that our ability to resolve different haplotypes was constrained by the low levels of diversity at this locus and our small amplicon length (478bp).Fig. 1


Presence of the knockdown resistance mutation, Vgsc-1014F in Anopheles gambiae and An. arabiensis in western Kenya.

Ochomo E, Subramaniam K, Kemei B, Rippon E, Bayoh NM, Kamau L, Atieli F, Vulule JM, Ouma C, Gimnig J, Donnelly MJ, Mbogo C - Parasit Vectors (2015)

A TCS plot of the three haplotypes present in the populations assayed. White colour represents An. gambiae while black colour represents An. arabiensis
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4666190&req=5

Fig1: A TCS plot of the three haplotypes present in the populations assayed. White colour represents An. gambiae while black colour represents An. arabiensis
Mentions: Only a single 1014F haplotype was observed (Fig. 1), suggesting a common origin in the species and subsequent interspecific transfer. However it should be noted that our ability to resolve different haplotypes was constrained by the low levels of diversity at this locus and our small amplicon length (478bp).Fig. 1

Bottom Line: A portion of the samples were submitted for sequencing in order to confirm the Vgsc-1014F genotyping results.The resulting sequence data were deposited in GenBank (Accession numbers: KR867642-KR867651, KT758295-KT758303).It is important that we start monitoring relative frequencies of the two kdr genes so that we can determine their relative importance in an area of high insecticide treated net ownership.

View Article: PubMed Central - PubMed

Affiliation: School of Public Health and Community Development, Maseno University, Maseno, Kenya. eochomo@kemricdc.org.

ABSTRACT

Introduction: The voltage gated sodium channel mutation Vgsc-1014S (kdr-east) was first reported in Kenya in 2000 and has since been observed to occur at high frequencies in the local Anopheles gambiae s.s.

Population: The mutation Vgsc-1014F has never been reported from An. gambiae Complex complex mosquitoes in Kenya.

Findings: Molecularly confirmed An. gambiae s.s. (hereafter An. gambiae) and An. arabiensis collected from 4 different parts of western Kenya were genotyped for kdr from 2011 to 2013. Vgsc-1014F was observed to have emerged, apparently, simultaneously in both An. gambiae and An. arabiensis in 2012. A portion of the samples were submitted for sequencing in order to confirm the Vgsc-1014F genotyping results. The resulting sequence data were deposited in GenBank (Accession numbers: KR867642-KR867651, KT758295-KT758303). A single Vgsc-1014F haplotype was observed suggesting, a common origin in both species.

Conclusion: This is the first report of Vgsc-1014F in Kenya. Based on our samples, the mutation is present in low frequencies in both An. gambiae and An. arabiensis. It is important that we start monitoring relative frequencies of the two kdr genes so that we can determine their relative importance in an area of high insecticide treated net ownership.

Show MeSH