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Differential expression of galanin in the cholinergic basal forebrain of patients with Lewy body disorders.

Alexandris A, Liu AK, Chang RC, Pearce RK, Gentleman SM - Acta Neuropathol Commun (2015)

Bottom Line: Gallatin immunohistochemistry was carried out on anterior nbM sections from 76 LBD cases (27 PD, 15 PD with mild cognitive impairment (MCI), 34 PD with dementia (PDD) and 4 aged-matched controls.The LBD group had significantly higher galaninergic innervation scores (p = 0.016) compared to controls.However, this difference was due to increased innervation density only in a subgroup of LBD cases and this correlated positively with choline acetyltransferase-immunopositive neuron density.

View Article: PubMed Central - PubMed

Affiliation: Division of Brain Sciences, Department of Medicine, Imperial College London, Burlington Danes Building, Hammersmith Hospital Campus, London, W12 0NN, UK. a.s.alexandris@gmail.com.

ABSTRACT

Introduction: Depletion of cholinergic neurons within the nucleus basalis of Meynert (nbM) is thought to contribute to the development of cognitive impairments in both Alzheimer's disease (AD) and Lewy body disorders (LBD). It has been reported that, in late stage AD, a network of fibres that contain the neuropeptide galanin displays significant hypertrophy and 'hyperinnervates' the surviving cholinergic neurons. Galanin is considered as a highly inducible neuroprotective factor and in AD this is assumed to be part of a protective tissue response. The aim of this study was to determine if a similar galanin upregulation is present in the nbM in post-mortem tissue from patients with LBD. Gallatin immunohistochemistry was carried out on anterior nbM sections from 76 LBD cases (27 PD, 15 PD with mild cognitive impairment (MCI), 34 PD with dementia (PDD) and 4 aged-matched controls. Galaninergic innervation of cholinergic neurons was assessed on a semi-quantitative scale.

Results: The LBD group had significantly higher galaninergic innervation scores (p = 0.016) compared to controls. However, this difference was due to increased innervation density only in a subgroup of LBD cases and this correlated positively with choline acetyltransferase-immunopositive neuron density.

Conclusion: Galanin upregulation within the basal forebrain cholinergic system in LBD, similar to that seen in AD, may represent an intrinsic adaptive response to neurodegeneration that is consistent with its proposed roles in neurogenesis and neuroprotection.

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a Semiquantitative assessment of galaninergic innervation of nucleus basalis of Meynert in Parkinson’s disease (PD) without or with mild cognitive impairment (PD-MCI), Parkinson’s disease dementia (PDD), and age-matched controls. Horizontal bars indicate the median value with interquartile range. b Percentage of cases displaying different innervation scores within each diagnostic category. For explanation of grading system see text
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Fig6: a Semiquantitative assessment of galaninergic innervation of nucleus basalis of Meynert in Parkinson’s disease (PD) without or with mild cognitive impairment (PD-MCI), Parkinson’s disease dementia (PDD), and age-matched controls. Horizontal bars indicate the median value with interquartile range. b Percentage of cases displaying different innervation scores within each diagnostic category. For explanation of grading system see text

Mentions: Semi-quantitative assessment of innervation density revealed that the extent of innervation was significantly higher in LBD (PD, PD-MCI and PDD combined) compared to the age-matched controls; U(78) = 57.00, Z = −2.219, exact p = 0.016 (one-tailed; see Fig. 6a). Further, analysis indicated no significant differences among the different diagnostic groups although it was noticed that the PD-MCI group had more cases displaying hyperinnervation compared to PD and PDD groups (Fig. 6b).Fig. 6


Differential expression of galanin in the cholinergic basal forebrain of patients with Lewy body disorders.

Alexandris A, Liu AK, Chang RC, Pearce RK, Gentleman SM - Acta Neuropathol Commun (2015)

a Semiquantitative assessment of galaninergic innervation of nucleus basalis of Meynert in Parkinson’s disease (PD) without or with mild cognitive impairment (PD-MCI), Parkinson’s disease dementia (PDD), and age-matched controls. Horizontal bars indicate the median value with interquartile range. b Percentage of cases displaying different innervation scores within each diagnostic category. For explanation of grading system see text
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4666186&req=5

Fig6: a Semiquantitative assessment of galaninergic innervation of nucleus basalis of Meynert in Parkinson’s disease (PD) without or with mild cognitive impairment (PD-MCI), Parkinson’s disease dementia (PDD), and age-matched controls. Horizontal bars indicate the median value with interquartile range. b Percentage of cases displaying different innervation scores within each diagnostic category. For explanation of grading system see text
Mentions: Semi-quantitative assessment of innervation density revealed that the extent of innervation was significantly higher in LBD (PD, PD-MCI and PDD combined) compared to the age-matched controls; U(78) = 57.00, Z = −2.219, exact p = 0.016 (one-tailed; see Fig. 6a). Further, analysis indicated no significant differences among the different diagnostic groups although it was noticed that the PD-MCI group had more cases displaying hyperinnervation compared to PD and PDD groups (Fig. 6b).Fig. 6

Bottom Line: Gallatin immunohistochemistry was carried out on anterior nbM sections from 76 LBD cases (27 PD, 15 PD with mild cognitive impairment (MCI), 34 PD with dementia (PDD) and 4 aged-matched controls.The LBD group had significantly higher galaninergic innervation scores (p = 0.016) compared to controls.However, this difference was due to increased innervation density only in a subgroup of LBD cases and this correlated positively with choline acetyltransferase-immunopositive neuron density.

View Article: PubMed Central - PubMed

Affiliation: Division of Brain Sciences, Department of Medicine, Imperial College London, Burlington Danes Building, Hammersmith Hospital Campus, London, W12 0NN, UK. a.s.alexandris@gmail.com.

ABSTRACT

Introduction: Depletion of cholinergic neurons within the nucleus basalis of Meynert (nbM) is thought to contribute to the development of cognitive impairments in both Alzheimer's disease (AD) and Lewy body disorders (LBD). It has been reported that, in late stage AD, a network of fibres that contain the neuropeptide galanin displays significant hypertrophy and 'hyperinnervates' the surviving cholinergic neurons. Galanin is considered as a highly inducible neuroprotective factor and in AD this is assumed to be part of a protective tissue response. The aim of this study was to determine if a similar galanin upregulation is present in the nbM in post-mortem tissue from patients with LBD. Gallatin immunohistochemistry was carried out on anterior nbM sections from 76 LBD cases (27 PD, 15 PD with mild cognitive impairment (MCI), 34 PD with dementia (PDD) and 4 aged-matched controls. Galaninergic innervation of cholinergic neurons was assessed on a semi-quantitative scale.

Results: The LBD group had significantly higher galaninergic innervation scores (p = 0.016) compared to controls. However, this difference was due to increased innervation density only in a subgroup of LBD cases and this correlated positively with choline acetyltransferase-immunopositive neuron density.

Conclusion: Galanin upregulation within the basal forebrain cholinergic system in LBD, similar to that seen in AD, may represent an intrinsic adaptive response to neurodegeneration that is consistent with its proposed roles in neurogenesis and neuroprotection.

Show MeSH
Related in: MedlinePlus