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Interictal cytokine levels were correlated to seizure severity of epileptic patients: a retrospective study on 1218 epileptic patients.

Wang Y, Wang D, Guo D - J Transl Med (2015)

Bottom Line: Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups.Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, Peoples' Republic of China. Yewang232@163.com.

ABSTRACT

Background: Many aspects on the correlation between epilepsy and cytokine levels were unclear. This study aims to investigate the correlations between cytokine levels and severe epilepsy.

Methods: Totally 1218 epileptic patients were grouped by types of epilepsy: TLE (temporal lobe epilepsy, n = 409), XLE (extra-temporal lobe epilepsy, n = 290) and IGE (idiopathic generalized epilepsy, n = 519). Two hundred healthy volunteers were as controls. Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.

Results: Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups. Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3. Multivariate regression analysis indicated that interictal concentrations of serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 were significant biomarkers for patients with severe epilepsy. mRNA levels of IL-6, IFNγ, IL-17a, and IFNλ3 were elevated in different types of epilepsy. Activation of all STATs was elevated in epilepsy, and STAT3 was activated 9-fold in average, which was the highest among all STATs.

Conclusions: Interictal serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 could be used as potential biomarkers for severe epilepsy. Activation of STATs, especially STAT3, was important in epilepsy. Our findings pointed out crucial roles of cytokine levels in epilepsy.

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STATs were activated in patients with different types of epilepsy. PBMCs were extracted from healthy controls and different epileptic patients (TLE, XLE and IGE). Levels of activated STAT1 (a), STAT2 (b), STAT3 (c), STAT4 (d), STAT5a (e), and STAT5b (f) were measured by flow cytometry. Data represent a minimum of three independent experiments. Asterisks denote p < 0.05 in comparison to untreated samples. TLE temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, IGE idiopathic generalized epilepsy
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Fig3: STATs were activated in patients with different types of epilepsy. PBMCs were extracted from healthy controls and different epileptic patients (TLE, XLE and IGE). Levels of activated STAT1 (a), STAT2 (b), STAT3 (c), STAT4 (d), STAT5a (e), and STAT5b (f) were measured by flow cytometry. Data represent a minimum of three independent experiments. Asterisks denote p < 0.05 in comparison to untreated samples. TLE temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, IGE idiopathic generalized epilepsy

Mentions: STATs are key signaling mediators induced by most interleukins and interferons [22]. The concentrations of activated all 6 STAT family members were evaluated in blood PBMCs of patients with different types of epilepsy. We found that activation of all tested STATs was significantly elevated in all three types of epilepsy. Among all STAT members, STAT3 activation level was increased ninefold in average, much higher than other STAT members (3–5 fold), indicating potential critical role of STAT3 in epilepsy (Fig. 3).Fig. 3


Interictal cytokine levels were correlated to seizure severity of epileptic patients: a retrospective study on 1218 epileptic patients.

Wang Y, Wang D, Guo D - J Transl Med (2015)

STATs were activated in patients with different types of epilepsy. PBMCs were extracted from healthy controls and different epileptic patients (TLE, XLE and IGE). Levels of activated STAT1 (a), STAT2 (b), STAT3 (c), STAT4 (d), STAT5a (e), and STAT5b (f) were measured by flow cytometry. Data represent a minimum of three independent experiments. Asterisks denote p < 0.05 in comparison to untreated samples. TLE temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, IGE idiopathic generalized epilepsy
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4666166&req=5

Fig3: STATs were activated in patients with different types of epilepsy. PBMCs were extracted from healthy controls and different epileptic patients (TLE, XLE and IGE). Levels of activated STAT1 (a), STAT2 (b), STAT3 (c), STAT4 (d), STAT5a (e), and STAT5b (f) were measured by flow cytometry. Data represent a minimum of three independent experiments. Asterisks denote p < 0.05 in comparison to untreated samples. TLE temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, IGE idiopathic generalized epilepsy
Mentions: STATs are key signaling mediators induced by most interleukins and interferons [22]. The concentrations of activated all 6 STAT family members were evaluated in blood PBMCs of patients with different types of epilepsy. We found that activation of all tested STATs was significantly elevated in all three types of epilepsy. Among all STAT members, STAT3 activation level was increased ninefold in average, much higher than other STAT members (3–5 fold), indicating potential critical role of STAT3 in epilepsy (Fig. 3).Fig. 3

Bottom Line: Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups.Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, Peoples' Republic of China. Yewang232@163.com.

ABSTRACT

Background: Many aspects on the correlation between epilepsy and cytokine levels were unclear. This study aims to investigate the correlations between cytokine levels and severe epilepsy.

Methods: Totally 1218 epileptic patients were grouped by types of epilepsy: TLE (temporal lobe epilepsy, n = 409), XLE (extra-temporal lobe epilepsy, n = 290) and IGE (idiopathic generalized epilepsy, n = 519). Two hundred healthy volunteers were as controls. Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.

Results: Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups. Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3. Multivariate regression analysis indicated that interictal concentrations of serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 were significant biomarkers for patients with severe epilepsy. mRNA levels of IL-6, IFNγ, IL-17a, and IFNλ3 were elevated in different types of epilepsy. Activation of all STATs was elevated in epilepsy, and STAT3 was activated 9-fold in average, which was the highest among all STATs.

Conclusions: Interictal serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 could be used as potential biomarkers for severe epilepsy. Activation of STATs, especially STAT3, was important in epilepsy. Our findings pointed out crucial roles of cytokine levels in epilepsy.

Show MeSH
Related in: MedlinePlus