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Interictal cytokine levels were correlated to seizure severity of epileptic patients: a retrospective study on 1218 epileptic patients.

Wang Y, Wang D, Guo D - J Transl Med (2015)

Bottom Line: Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups.Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, Peoples' Republic of China. Yewang232@163.com.

ABSTRACT

Background: Many aspects on the correlation between epilepsy and cytokine levels were unclear. This study aims to investigate the correlations between cytokine levels and severe epilepsy.

Methods: Totally 1218 epileptic patients were grouped by types of epilepsy: TLE (temporal lobe epilepsy, n = 409), XLE (extra-temporal lobe epilepsy, n = 290) and IGE (idiopathic generalized epilepsy, n = 519). Two hundred healthy volunteers were as controls. Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.

Results: Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups. Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3. Multivariate regression analysis indicated that interictal concentrations of serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 were significant biomarkers for patients with severe epilepsy. mRNA levels of IL-6, IFNγ, IL-17a, and IFNλ3 were elevated in different types of epilepsy. Activation of all STATs was elevated in epilepsy, and STAT3 was activated 9-fold in average, which was the highest among all STATs.

Conclusions: Interictal serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 could be used as potential biomarkers for severe epilepsy. Activation of STATs, especially STAT3, was important in epilepsy. Our findings pointed out crucial roles of cytokine levels in epilepsy.

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Serum mRNA concentration of IL-6, IL-17a, IFNγ and IFNλ3 were elevated in epilepsy. Serum mRNA concentration of IL-6 (a), IL-17a (b), IFNγ (c) and IFNλ3 (d) were measured in healthy controls and patients with TLE, XLE or IGE. Data represent a minimum of three independent experiments. Asterisks denote p < 0.05 in comparison to untreated samples. TLE: temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, IGE idiopathic generalized epilepsy
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Fig2: Serum mRNA concentration of IL-6, IL-17a, IFNγ and IFNλ3 were elevated in epilepsy. Serum mRNA concentration of IL-6 (a), IL-17a (b), IFNγ (c) and IFNλ3 (d) were measured in healthy controls and patients with TLE, XLE or IGE. Data represent a minimum of three independent experiments. Asterisks denote p < 0.05 in comparison to untreated samples. TLE: temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, IGE idiopathic generalized epilepsy

Mentions: The mRNA concentrations of 14 tested cytokines were evaluated, and the data on IL-6, IL-17a, IFNγ and IFNλ3 were shown in Fig. 2, since these four cytokines were identified in the multivariate analysis (Table 4). The mRNA levels of all these four cytokines were correlated to all three types of epilepsy. No difference was observed between types of epilepsy (all P > 0.05).Fig. 2


Interictal cytokine levels were correlated to seizure severity of epileptic patients: a retrospective study on 1218 epileptic patients.

Wang Y, Wang D, Guo D - J Transl Med (2015)

Serum mRNA concentration of IL-6, IL-17a, IFNγ and IFNλ3 were elevated in epilepsy. Serum mRNA concentration of IL-6 (a), IL-17a (b), IFNγ (c) and IFNλ3 (d) were measured in healthy controls and patients with TLE, XLE or IGE. Data represent a minimum of three independent experiments. Asterisks denote p < 0.05 in comparison to untreated samples. TLE: temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, IGE idiopathic generalized epilepsy
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4666166&req=5

Fig2: Serum mRNA concentration of IL-6, IL-17a, IFNγ and IFNλ3 were elevated in epilepsy. Serum mRNA concentration of IL-6 (a), IL-17a (b), IFNγ (c) and IFNλ3 (d) were measured in healthy controls and patients with TLE, XLE or IGE. Data represent a minimum of three independent experiments. Asterisks denote p < 0.05 in comparison to untreated samples. TLE: temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, IGE idiopathic generalized epilepsy
Mentions: The mRNA concentrations of 14 tested cytokines were evaluated, and the data on IL-6, IL-17a, IFNγ and IFNλ3 were shown in Fig. 2, since these four cytokines were identified in the multivariate analysis (Table 4). The mRNA levels of all these four cytokines were correlated to all three types of epilepsy. No difference was observed between types of epilepsy (all P > 0.05).Fig. 2

Bottom Line: Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups.Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, Peoples' Republic of China. Yewang232@163.com.

ABSTRACT

Background: Many aspects on the correlation between epilepsy and cytokine levels were unclear. This study aims to investigate the correlations between cytokine levels and severe epilepsy.

Methods: Totally 1218 epileptic patients were grouped by types of epilepsy: TLE (temporal lobe epilepsy, n = 409), XLE (extra-temporal lobe epilepsy, n = 290) and IGE (idiopathic generalized epilepsy, n = 519). Two hundred healthy volunteers were as controls. Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.

Results: Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups. Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3. Multivariate regression analysis indicated that interictal concentrations of serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 were significant biomarkers for patients with severe epilepsy. mRNA levels of IL-6, IFNγ, IL-17a, and IFNλ3 were elevated in different types of epilepsy. Activation of all STATs was elevated in epilepsy, and STAT3 was activated 9-fold in average, which was the highest among all STATs.

Conclusions: Interictal serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 could be used as potential biomarkers for severe epilepsy. Activation of STATs, especially STAT3, was important in epilepsy. Our findings pointed out crucial roles of cytokine levels in epilepsy.

Show MeSH
Related in: MedlinePlus