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Interictal cytokine levels were correlated to seizure severity of epileptic patients: a retrospective study on 1218 epileptic patients.

Wang Y, Wang D, Guo D - J Transl Med (2015)

Bottom Line: Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups.Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, Peoples' Republic of China. Yewang232@163.com.

ABSTRACT

Background: Many aspects on the correlation between epilepsy and cytokine levels were unclear. This study aims to investigate the correlations between cytokine levels and severe epilepsy.

Methods: Totally 1218 epileptic patients were grouped by types of epilepsy: TLE (temporal lobe epilepsy, n = 409), XLE (extra-temporal lobe epilepsy, n = 290) and IGE (idiopathic generalized epilepsy, n = 519). Two hundred healthy volunteers were as controls. Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.

Results: Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups. Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3. Multivariate regression analysis indicated that interictal concentrations of serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 were significant biomarkers for patients with severe epilepsy. mRNA levels of IL-6, IFNγ, IL-17a, and IFNλ3 were elevated in different types of epilepsy. Activation of all STATs was elevated in epilepsy, and STAT3 was activated 9-fold in average, which was the highest among all STATs.

Conclusions: Interictal serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 could be used as potential biomarkers for severe epilepsy. Activation of STATs, especially STAT3, was important in epilepsy. Our findings pointed out crucial roles of cytokine levels in epilepsy.

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Correlation web of biomarkers and severity indices for three types of epilepsies. The cross-correlation of cytokine levels with seizure indices for three types of epilepsies was shown in the form of directed correlation web. The line thickness indicates the significance of the correlations. Bold line The statistical correlation is <0.05 in multivariate analysis; Thin line The statistical correlation is <0.01 in univariate analysis; Dash line: The statistical correlation is <0.01 in univariate analysis. TLE temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, and IGE idiopathic generalized epilepsy
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Fig1: Correlation web of biomarkers and severity indices for three types of epilepsies. The cross-correlation of cytokine levels with seizure indices for three types of epilepsies was shown in the form of directed correlation web. The line thickness indicates the significance of the correlations. Bold line The statistical correlation is <0.05 in multivariate analysis; Thin line The statistical correlation is <0.01 in univariate analysis; Dash line: The statistical correlation is <0.01 in univariate analysis. TLE temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, and IGE idiopathic generalized epilepsy

Mentions: Furthermore, multivariate regression analysis revealed that interictal IL-6 concentration was positively related to seizure frequency in TLE and IGE (P = 0.041 and 0.002, respectively), VA score in IGE (P < 0.001), and NHS3 in XLE (P = 0.005). IFNγ level was positively linked to VA score in XLE (P = 0.015), and NHS3 in TLE (P = 0.023). IL-17a level was positively associated with VA score in TLE and IGE (P = 0.010 and 0.022, respectively). IFNλ3 concentration was positively linked to NHS3 in TLE and IGE (P = 0.019 and 0.022), and seizure frequency in IGE (P = 0.034) (Table 4). The overall data of statistical analysis on correlations between seizure severity and cytokine levels in TLE, XLE and IGE were shown graphically in Fig. 1. If P < 0.01 was considered significant, then only IL-6 concentration was positively linked to NHS3 in XLE (P = 0.005), seizure frequency and VA score in IGE (P = 0.002 and <0.001, respectively).Table 4


Interictal cytokine levels were correlated to seizure severity of epileptic patients: a retrospective study on 1218 epileptic patients.

Wang Y, Wang D, Guo D - J Transl Med (2015)

Correlation web of biomarkers and severity indices for three types of epilepsies. The cross-correlation of cytokine levels with seizure indices for three types of epilepsies was shown in the form of directed correlation web. The line thickness indicates the significance of the correlations. Bold line The statistical correlation is <0.05 in multivariate analysis; Thin line The statistical correlation is <0.01 in univariate analysis; Dash line: The statistical correlation is <0.01 in univariate analysis. TLE temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, and IGE idiopathic generalized epilepsy
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4666166&req=5

Fig1: Correlation web of biomarkers and severity indices for three types of epilepsies. The cross-correlation of cytokine levels with seizure indices for three types of epilepsies was shown in the form of directed correlation web. The line thickness indicates the significance of the correlations. Bold line The statistical correlation is <0.05 in multivariate analysis; Thin line The statistical correlation is <0.01 in univariate analysis; Dash line: The statistical correlation is <0.01 in univariate analysis. TLE temporal lobe epilepsy, XLE extra-temporal lobe epilepsy, and IGE idiopathic generalized epilepsy
Mentions: Furthermore, multivariate regression analysis revealed that interictal IL-6 concentration was positively related to seizure frequency in TLE and IGE (P = 0.041 and 0.002, respectively), VA score in IGE (P < 0.001), and NHS3 in XLE (P = 0.005). IFNγ level was positively linked to VA score in XLE (P = 0.015), and NHS3 in TLE (P = 0.023). IL-17a level was positively associated with VA score in TLE and IGE (P = 0.010 and 0.022, respectively). IFNλ3 concentration was positively linked to NHS3 in TLE and IGE (P = 0.019 and 0.022), and seizure frequency in IGE (P = 0.034) (Table 4). The overall data of statistical analysis on correlations between seizure severity and cytokine levels in TLE, XLE and IGE were shown graphically in Fig. 1. If P < 0.01 was considered significant, then only IL-6 concentration was positively linked to NHS3 in XLE (P = 0.005), seizure frequency and VA score in IGE (P = 0.002 and <0.001, respectively).Table 4

Bottom Line: Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups.Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, Peoples' Republic of China. Yewang232@163.com.

ABSTRACT

Background: Many aspects on the correlation between epilepsy and cytokine levels were unclear. This study aims to investigate the correlations between cytokine levels and severe epilepsy.

Methods: Totally 1218 epileptic patients were grouped by types of epilepsy: TLE (temporal lobe epilepsy, n = 409), XLE (extra-temporal lobe epilepsy, n = 290) and IGE (idiopathic generalized epilepsy, n = 519). Two hundred healthy volunteers were as controls. Clinical findings and levels of 14 serum and CSF cytokines and 6 STAT members were collected, measured and analyzed.

Results: Analysis showed no differences in interictal cytokine levels among patients from TLE, XLE and IGE groups. Interictal serum levels of IL-1b, IL-1Ra, IL-6, IL-8, IFNγ, IFNλ3 and IL-17a were associated with seizure severity of epileptic patients, measured by seizure frequency, VA score or NHS3. Multivariate regression analysis indicated that interictal concentrations of serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 were significant biomarkers for patients with severe epilepsy. mRNA levels of IL-6, IFNγ, IL-17a, and IFNλ3 were elevated in different types of epilepsy. Activation of all STATs was elevated in epilepsy, and STAT3 was activated 9-fold in average, which was the highest among all STATs.

Conclusions: Interictal serum IL-6, IFNγ, IL-17a, IFNλ3, and CSF IL-6, IL-17a, IFNλ3 could be used as potential biomarkers for severe epilepsy. Activation of STATs, especially STAT3, was important in epilepsy. Our findings pointed out crucial roles of cytokine levels in epilepsy.

Show MeSH
Related in: MedlinePlus