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Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis.

Feng C, Li B, Wang LL, Chen LI, Zhou X, Lv FQ, Li TS - Exp Ther Med (2015)

Bottom Line: The levels of serum inflammatory mediators were effectively reduced in groups SL, IU and UL, the greatest effects were observed in group UL.The expression levels of NF-κB and TNF-α in multiple organs were significantly lower in group UL compared with other groups.Intraperitoneal lavage with ulinastatin significantly ameliorated the inflammatory reaction and inhibited NF-κB and TNF-α expression in multiple organs of SAP model rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, PLA General Hospital, Beijing 100853, P.R. China ; Li-Shi Road Outpatient Department, Second Artillery General Hospital of the PLA, Beijing 100820, P.R. China.

ABSTRACT

The aim of the present study was to investigate the effect of peritoneal lavage with ulinastatin on the expression levels of nuclear factor κB (NF-κB) and tumor necrosis factor (TNF)-α in multiple organs of rats with severe acute pancreatitis (SAP). Male Wistar rats were randomly divided into the following groups: Sham-operated (C), SAP model (SAP), saline lavage (SL), intravenous ulinastatin (IU) and peritoneal lavage with ulinastatin (UL). The SAP model was induced by the retrograde infusion of 5% sodium taurocholate into the biliopancreatic ducts of the rats. Intraperitoneal lavage or injection was performed immediately following the establishment of the SAP model in groups SL, IU and UL. Intraperitoneal lavage with or without ulinastatin was performed for 3 h. The survival time of half of the rats in each group was recorded over a 12-h period. At 3 h after the induction of SAP, inflammatory mediators and the expression levels of NF-κB and TNF-α in multiple organs of the rats in each group were also detected. The survival rates of the rats in group UL at 6 h and 9 h were increased compared with those in group SAP, and were also higher than that in groups SL and IU. The levels of serum inflammatory mediators were effectively reduced in groups SL, IU and UL, the greatest effects were observed in group UL. The expression levels of NF-κB and TNF-α in multiple organs were significantly lower in group UL compared with other groups. Intraperitoneal lavage with ulinastatin significantly ameliorated the inflammatory reaction and inhibited NF-κB and TNF-α expression in multiple organs of SAP model rats.

No MeSH data available.


Related in: MedlinePlus

Comparison of different treatments on the expression levels of TNF-α in the (A) pancreas, (B) lung, (C) liver and (D) kidney. The expression levels of TNF-α in multiple organs were greatly increased in group SAP compared with those in group C. The treatments administered to groups SL, IU and UL all significantly decreased the expression levels of TNF-α in multiple organs compared with those in group SAP and group UL exhibited the greatest reduction in expression levels. *P<0.05, **P<0.01. C, control; SAP, severe acute pancreatitis; SL, saline lavage; IU, intravenous ulinastatin; UL, ulinastatin lavage; TNF-α, tumor necrosis factor-α.
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f2-etm-0-0-2802: Comparison of different treatments on the expression levels of TNF-α in the (A) pancreas, (B) lung, (C) liver and (D) kidney. The expression levels of TNF-α in multiple organs were greatly increased in group SAP compared with those in group C. The treatments administered to groups SL, IU and UL all significantly decreased the expression levels of TNF-α in multiple organs compared with those in group SAP and group UL exhibited the greatest reduction in expression levels. *P<0.05, **P<0.01. C, control; SAP, severe acute pancreatitis; SL, saline lavage; IU, intravenous ulinastatin; UL, ulinastatin lavage; TNF-α, tumor necrosis factor-α.

Mentions: The expression levels of TNF-α in the pancreas, lung, liver and kidney of rats in group SAP were significantly increased compared with those in group C. In groups SL, IU and UL, the expression levels of TNF-α in the pancreas were significantly reduced compared with those in group SAP, and groups SL and UL exhibited greater reductions in TNF-α levels than group IU (Fig. 2A). The expression levels of TNF-α in the lungs of rats in groups SL, IU and UL were significantly reduced compared with those in group SAP, and the TNF-α levels in group UL were the lowest among these three groups (Fig. 2B). The expression levels of TNF-α in the livers of the rats in groups SL, IU and UL were significantly reduced compared with those in group SAP. The effects in groups UL and SL were comparable and stronger than those in group IU (Fig. 2C). The expression levels of TNF-α in the kidneys of the rats in groups SL, IU and UL were significantly reduced compared with those in group SAP, and were reduced to the greatest extent in group UL (Fig. 2D). The results are summarized in Table III.


Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis.

Feng C, Li B, Wang LL, Chen LI, Zhou X, Lv FQ, Li TS - Exp Ther Med (2015)

Comparison of different treatments on the expression levels of TNF-α in the (A) pancreas, (B) lung, (C) liver and (D) kidney. The expression levels of TNF-α in multiple organs were greatly increased in group SAP compared with those in group C. The treatments administered to groups SL, IU and UL all significantly decreased the expression levels of TNF-α in multiple organs compared with those in group SAP and group UL exhibited the greatest reduction in expression levels. *P<0.05, **P<0.01. C, control; SAP, severe acute pancreatitis; SL, saline lavage; IU, intravenous ulinastatin; UL, ulinastatin lavage; TNF-α, tumor necrosis factor-α.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4665968&req=5

f2-etm-0-0-2802: Comparison of different treatments on the expression levels of TNF-α in the (A) pancreas, (B) lung, (C) liver and (D) kidney. The expression levels of TNF-α in multiple organs were greatly increased in group SAP compared with those in group C. The treatments administered to groups SL, IU and UL all significantly decreased the expression levels of TNF-α in multiple organs compared with those in group SAP and group UL exhibited the greatest reduction in expression levels. *P<0.05, **P<0.01. C, control; SAP, severe acute pancreatitis; SL, saline lavage; IU, intravenous ulinastatin; UL, ulinastatin lavage; TNF-α, tumor necrosis factor-α.
Mentions: The expression levels of TNF-α in the pancreas, lung, liver and kidney of rats in group SAP were significantly increased compared with those in group C. In groups SL, IU and UL, the expression levels of TNF-α in the pancreas were significantly reduced compared with those in group SAP, and groups SL and UL exhibited greater reductions in TNF-α levels than group IU (Fig. 2A). The expression levels of TNF-α in the lungs of rats in groups SL, IU and UL were significantly reduced compared with those in group SAP, and the TNF-α levels in group UL were the lowest among these three groups (Fig. 2B). The expression levels of TNF-α in the livers of the rats in groups SL, IU and UL were significantly reduced compared with those in group SAP. The effects in groups UL and SL were comparable and stronger than those in group IU (Fig. 2C). The expression levels of TNF-α in the kidneys of the rats in groups SL, IU and UL were significantly reduced compared with those in group SAP, and were reduced to the greatest extent in group UL (Fig. 2D). The results are summarized in Table III.

Bottom Line: The levels of serum inflammatory mediators were effectively reduced in groups SL, IU and UL, the greatest effects were observed in group UL.The expression levels of NF-κB and TNF-α in multiple organs were significantly lower in group UL compared with other groups.Intraperitoneal lavage with ulinastatin significantly ameliorated the inflammatory reaction and inhibited NF-κB and TNF-α expression in multiple organs of SAP model rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, PLA General Hospital, Beijing 100853, P.R. China ; Li-Shi Road Outpatient Department, Second Artillery General Hospital of the PLA, Beijing 100820, P.R. China.

ABSTRACT

The aim of the present study was to investigate the effect of peritoneal lavage with ulinastatin on the expression levels of nuclear factor κB (NF-κB) and tumor necrosis factor (TNF)-α in multiple organs of rats with severe acute pancreatitis (SAP). Male Wistar rats were randomly divided into the following groups: Sham-operated (C), SAP model (SAP), saline lavage (SL), intravenous ulinastatin (IU) and peritoneal lavage with ulinastatin (UL). The SAP model was induced by the retrograde infusion of 5% sodium taurocholate into the biliopancreatic ducts of the rats. Intraperitoneal lavage or injection was performed immediately following the establishment of the SAP model in groups SL, IU and UL. Intraperitoneal lavage with or without ulinastatin was performed for 3 h. The survival time of half of the rats in each group was recorded over a 12-h period. At 3 h after the induction of SAP, inflammatory mediators and the expression levels of NF-κB and TNF-α in multiple organs of the rats in each group were also detected. The survival rates of the rats in group UL at 6 h and 9 h were increased compared with those in group SAP, and were also higher than that in groups SL and IU. The levels of serum inflammatory mediators were effectively reduced in groups SL, IU and UL, the greatest effects were observed in group UL. The expression levels of NF-κB and TNF-α in multiple organs were significantly lower in group UL compared with other groups. Intraperitoneal lavage with ulinastatin significantly ameliorated the inflammatory reaction and inhibited NF-κB and TNF-α expression in multiple organs of SAP model rats.

No MeSH data available.


Related in: MedlinePlus