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Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis.

Feng C, Li B, Wang LL, Chen LI, Zhou X, Lv FQ, Li TS - Exp Ther Med (2015)

Bottom Line: The levels of serum inflammatory mediators were effectively reduced in groups SL, IU and UL, the greatest effects were observed in group UL.The expression levels of NF-κB and TNF-α in multiple organs were significantly lower in group UL compared with other groups.Intraperitoneal lavage with ulinastatin significantly ameliorated the inflammatory reaction and inhibited NF-κB and TNF-α expression in multiple organs of SAP model rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, PLA General Hospital, Beijing 100853, P.R. China ; Li-Shi Road Outpatient Department, Second Artillery General Hospital of the PLA, Beijing 100820, P.R. China.

ABSTRACT

The aim of the present study was to investigate the effect of peritoneal lavage with ulinastatin on the expression levels of nuclear factor κB (NF-κB) and tumor necrosis factor (TNF)-α in multiple organs of rats with severe acute pancreatitis (SAP). Male Wistar rats were randomly divided into the following groups: Sham-operated (C), SAP model (SAP), saline lavage (SL), intravenous ulinastatin (IU) and peritoneal lavage with ulinastatin (UL). The SAP model was induced by the retrograde infusion of 5% sodium taurocholate into the biliopancreatic ducts of the rats. Intraperitoneal lavage or injection was performed immediately following the establishment of the SAP model in groups SL, IU and UL. Intraperitoneal lavage with or without ulinastatin was performed for 3 h. The survival time of half of the rats in each group was recorded over a 12-h period. At 3 h after the induction of SAP, inflammatory mediators and the expression levels of NF-κB and TNF-α in multiple organs of the rats in each group were also detected. The survival rates of the rats in group UL at 6 h and 9 h were increased compared with those in group SAP, and were also higher than that in groups SL and IU. The levels of serum inflammatory mediators were effectively reduced in groups SL, IU and UL, the greatest effects were observed in group UL. The expression levels of NF-κB and TNF-α in multiple organs were significantly lower in group UL compared with other groups. Intraperitoneal lavage with ulinastatin significantly ameliorated the inflammatory reaction and inhibited NF-κB and TNF-α expression in multiple organs of SAP model rats.

No MeSH data available.


Related in: MedlinePlus

Effect of peritoneal lavage with ulinastatin on inflammatory mediators. The levels of (A) IL-1 and (B) IL-6 in group SAP were significantly increased compared with those in group C. Groups SL, IU and UL all exhibited reduced levels of IL-1 and IL-6 compared with those in group SAP. Moreover, group UL had lower levels of IL-1 and IL-6 than groups SL and IU. *P<0.05, **P<0.01. C, control; SAP, severe acute pancreatitis; SL, saline lavage; IU, intravenous ulinastatin; UL, ulinastatin lavage; IL, interleukin.
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f1-etm-0-0-2802: Effect of peritoneal lavage with ulinastatin on inflammatory mediators. The levels of (A) IL-1 and (B) IL-6 in group SAP were significantly increased compared with those in group C. Groups SL, IU and UL all exhibited reduced levels of IL-1 and IL-6 compared with those in group SAP. Moreover, group UL had lower levels of IL-1 and IL-6 than groups SL and IU. *P<0.05, **P<0.01. C, control; SAP, severe acute pancreatitis; SL, saline lavage; IU, intravenous ulinastatin; UL, ulinastatin lavage; IL, interleukin.

Mentions: The plasma levels of IL-1 and IL-6 in the rats of group SAP were significantly increased compared with those in group C. The levels of IL-1 in groups SL, IU and UL were significantly reduced compared with those in group SAP. The lowest levels of IL-1 were observed in group UL (Fig. 1). Compared with the IL-6 levels in group SAP, those in groups IU and UL were significantly reduced, and the strongest effect was observed in group UL, but group SL did not exhibit a statistically significant difference (Fig. 1). The results are summarized in Table II.


Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis.

Feng C, Li B, Wang LL, Chen LI, Zhou X, Lv FQ, Li TS - Exp Ther Med (2015)

Effect of peritoneal lavage with ulinastatin on inflammatory mediators. The levels of (A) IL-1 and (B) IL-6 in group SAP were significantly increased compared with those in group C. Groups SL, IU and UL all exhibited reduced levels of IL-1 and IL-6 compared with those in group SAP. Moreover, group UL had lower levels of IL-1 and IL-6 than groups SL and IU. *P<0.05, **P<0.01. C, control; SAP, severe acute pancreatitis; SL, saline lavage; IU, intravenous ulinastatin; UL, ulinastatin lavage; IL, interleukin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4665968&req=5

f1-etm-0-0-2802: Effect of peritoneal lavage with ulinastatin on inflammatory mediators. The levels of (A) IL-1 and (B) IL-6 in group SAP were significantly increased compared with those in group C. Groups SL, IU and UL all exhibited reduced levels of IL-1 and IL-6 compared with those in group SAP. Moreover, group UL had lower levels of IL-1 and IL-6 than groups SL and IU. *P<0.05, **P<0.01. C, control; SAP, severe acute pancreatitis; SL, saline lavage; IU, intravenous ulinastatin; UL, ulinastatin lavage; IL, interleukin.
Mentions: The plasma levels of IL-1 and IL-6 in the rats of group SAP were significantly increased compared with those in group C. The levels of IL-1 in groups SL, IU and UL were significantly reduced compared with those in group SAP. The lowest levels of IL-1 were observed in group UL (Fig. 1). Compared with the IL-6 levels in group SAP, those in groups IU and UL were significantly reduced, and the strongest effect was observed in group UL, but group SL did not exhibit a statistically significant difference (Fig. 1). The results are summarized in Table II.

Bottom Line: The levels of serum inflammatory mediators were effectively reduced in groups SL, IU and UL, the greatest effects were observed in group UL.The expression levels of NF-κB and TNF-α in multiple organs were significantly lower in group UL compared with other groups.Intraperitoneal lavage with ulinastatin significantly ameliorated the inflammatory reaction and inhibited NF-κB and TNF-α expression in multiple organs of SAP model rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, PLA General Hospital, Beijing 100853, P.R. China ; Li-Shi Road Outpatient Department, Second Artillery General Hospital of the PLA, Beijing 100820, P.R. China.

ABSTRACT

The aim of the present study was to investigate the effect of peritoneal lavage with ulinastatin on the expression levels of nuclear factor κB (NF-κB) and tumor necrosis factor (TNF)-α in multiple organs of rats with severe acute pancreatitis (SAP). Male Wistar rats were randomly divided into the following groups: Sham-operated (C), SAP model (SAP), saline lavage (SL), intravenous ulinastatin (IU) and peritoneal lavage with ulinastatin (UL). The SAP model was induced by the retrograde infusion of 5% sodium taurocholate into the biliopancreatic ducts of the rats. Intraperitoneal lavage or injection was performed immediately following the establishment of the SAP model in groups SL, IU and UL. Intraperitoneal lavage with or without ulinastatin was performed for 3 h. The survival time of half of the rats in each group was recorded over a 12-h period. At 3 h after the induction of SAP, inflammatory mediators and the expression levels of NF-κB and TNF-α in multiple organs of the rats in each group were also detected. The survival rates of the rats in group UL at 6 h and 9 h were increased compared with those in group SAP, and were also higher than that in groups SL and IU. The levels of serum inflammatory mediators were effectively reduced in groups SL, IU and UL, the greatest effects were observed in group UL. The expression levels of NF-κB and TNF-α in multiple organs were significantly lower in group UL compared with other groups. Intraperitoneal lavage with ulinastatin significantly ameliorated the inflammatory reaction and inhibited NF-κB and TNF-α expression in multiple organs of SAP model rats.

No MeSH data available.


Related in: MedlinePlus