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Effect of Age and Refractive Error on the Melanopsin Mediated Post-Illumination Pupil Response (PIPR).

Adhikari P, Pearson CA, Anderson AM, Zele AJ, Feigl B - Sci Rep (2015)

Bottom Line: Melanopsin containing intrinsically photosensitive Retinal Ganglion cells (ipRGCs) mediate the pupil light reflex (PLR) during light onset and at light offset (the post-illumination pupil response, PIPR).Recent evidence shows that the PLR and PIPR can provide non-invasive, objective markers of age-related retinal and optic nerve disease; however there is no consensus on the effects of healthy ageing or refractive error on the ipRGC mediated pupil function.Here we isolated melanopsin contributions to the pupil control pathway in 59 human participants with no ocular pathology across a range of ages and refractive errors.

View Article: PubMed Central - PubMed

Affiliation: Medical Retina and Visual Science Laboratories, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Brisbane QLD 4059, Australia.

ABSTRACT
Melanopsin containing intrinsically photosensitive Retinal Ganglion cells (ipRGCs) mediate the pupil light reflex (PLR) during light onset and at light offset (the post-illumination pupil response, PIPR). Recent evidence shows that the PLR and PIPR can provide non-invasive, objective markers of age-related retinal and optic nerve disease; however there is no consensus on the effects of healthy ageing or refractive error on the ipRGC mediated pupil function. Here we isolated melanopsin contributions to the pupil control pathway in 59 human participants with no ocular pathology across a range of ages and refractive errors. We show that there is no effect of age or refractive error on ipRGC inputs to the human pupil control pathway. The stability of the ipRGC mediated pupil response across the human lifespan provides a functional correlate of their robustness observed during ageing in rodent models.

No MeSH data available.


Related in: MedlinePlus

Panel (A) Scatterplot showing the peak pupil constriction (filled blue circles) and 6 s PIPR amplitude (open blue circles) for blue light as a function of refractive error (spherical equivalent, Dioptre, D).Panel (B): Median ± SD peak pupil constriction (filled symbols) and 6 s PIPR amplitude (open symbols) with red (red symbols) and blue (blue symbols) light pulses for myopes (triangles), emmetropes (circles), and hyperopes (squares). Panel (C): Scatterplot showing the bootstrapped estimates (B = 1560) of the data in Panel A; the solid lines indicate the best-fitting linear regressions.
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f7: Panel (A) Scatterplot showing the peak pupil constriction (filled blue circles) and 6 s PIPR amplitude (open blue circles) for blue light as a function of refractive error (spherical equivalent, Dioptre, D).Panel (B): Median ± SD peak pupil constriction (filled symbols) and 6 s PIPR amplitude (open symbols) with red (red symbols) and blue (blue symbols) light pulses for myopes (triangles), emmetropes (circles), and hyperopes (squares). Panel (C): Scatterplot showing the bootstrapped estimates (B = 1560) of the data in Panel A; the solid lines indicate the best-fitting linear regressions.

Mentions: The refractive status of 39 participants was known and the refractive error ranged from +3.00 D to −9.25 D (spherical equivalent). Due to a small sample of refractive errors, we bootstrapped the data to obtain an estimate of the sampling distribution for regression analysis (see Statistical analysis). The slopes of the best-fitting linear regression lines to the bootstrapped data (Fig. 7C) were not significantly different from zero indicating that the peak constriction and 6 s PIPR metrics have no relationship to refractive error. To determine the effect of refractive error status on the PLR and PIPR amplitudes, the participants were divided into three sub-groups on the basis of refractive error: emmetropes (< ±0.5 D) (n = 23), hyperopes (≥ +0.5 D) (n = 3), and myopes (≥−0.5 D) (n = 13) (Fig. 7B). Between the sub-groups, there was no significant difference in the peak pupil constriction amplitude (Kruskal-Wallis test; blue light: H = 0.77, P = 0.68; red light: H = 0.35, P = 0.84) or the 6 s PIPR amplitude (blue light: H = 1.84, P = 0.40, red light: H = 4.36, P = 0.11). The transient PLR (data not shown) was also unaffected by refractive status.


Effect of Age and Refractive Error on the Melanopsin Mediated Post-Illumination Pupil Response (PIPR).

Adhikari P, Pearson CA, Anderson AM, Zele AJ, Feigl B - Sci Rep (2015)

Panel (A) Scatterplot showing the peak pupil constriction (filled blue circles) and 6 s PIPR amplitude (open blue circles) for blue light as a function of refractive error (spherical equivalent, Dioptre, D).Panel (B): Median ± SD peak pupil constriction (filled symbols) and 6 s PIPR amplitude (open symbols) with red (red symbols) and blue (blue symbols) light pulses for myopes (triangles), emmetropes (circles), and hyperopes (squares). Panel (C): Scatterplot showing the bootstrapped estimates (B = 1560) of the data in Panel A; the solid lines indicate the best-fitting linear regressions.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4664956&req=5

f7: Panel (A) Scatterplot showing the peak pupil constriction (filled blue circles) and 6 s PIPR amplitude (open blue circles) for blue light as a function of refractive error (spherical equivalent, Dioptre, D).Panel (B): Median ± SD peak pupil constriction (filled symbols) and 6 s PIPR amplitude (open symbols) with red (red symbols) and blue (blue symbols) light pulses for myopes (triangles), emmetropes (circles), and hyperopes (squares). Panel (C): Scatterplot showing the bootstrapped estimates (B = 1560) of the data in Panel A; the solid lines indicate the best-fitting linear regressions.
Mentions: The refractive status of 39 participants was known and the refractive error ranged from +3.00 D to −9.25 D (spherical equivalent). Due to a small sample of refractive errors, we bootstrapped the data to obtain an estimate of the sampling distribution for regression analysis (see Statistical analysis). The slopes of the best-fitting linear regression lines to the bootstrapped data (Fig. 7C) were not significantly different from zero indicating that the peak constriction and 6 s PIPR metrics have no relationship to refractive error. To determine the effect of refractive error status on the PLR and PIPR amplitudes, the participants were divided into three sub-groups on the basis of refractive error: emmetropes (< ±0.5 D) (n = 23), hyperopes (≥ +0.5 D) (n = 3), and myopes (≥−0.5 D) (n = 13) (Fig. 7B). Between the sub-groups, there was no significant difference in the peak pupil constriction amplitude (Kruskal-Wallis test; blue light: H = 0.77, P = 0.68; red light: H = 0.35, P = 0.84) or the 6 s PIPR amplitude (blue light: H = 1.84, P = 0.40, red light: H = 4.36, P = 0.11). The transient PLR (data not shown) was also unaffected by refractive status.

Bottom Line: Melanopsin containing intrinsically photosensitive Retinal Ganglion cells (ipRGCs) mediate the pupil light reflex (PLR) during light onset and at light offset (the post-illumination pupil response, PIPR).Recent evidence shows that the PLR and PIPR can provide non-invasive, objective markers of age-related retinal and optic nerve disease; however there is no consensus on the effects of healthy ageing or refractive error on the ipRGC mediated pupil function.Here we isolated melanopsin contributions to the pupil control pathway in 59 human participants with no ocular pathology across a range of ages and refractive errors.

View Article: PubMed Central - PubMed

Affiliation: Medical Retina and Visual Science Laboratories, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Brisbane QLD 4059, Australia.

ABSTRACT
Melanopsin containing intrinsically photosensitive Retinal Ganglion cells (ipRGCs) mediate the pupil light reflex (PLR) during light onset and at light offset (the post-illumination pupil response, PIPR). Recent evidence shows that the PLR and PIPR can provide non-invasive, objective markers of age-related retinal and optic nerve disease; however there is no consensus on the effects of healthy ageing or refractive error on the ipRGC mediated pupil function. Here we isolated melanopsin contributions to the pupil control pathway in 59 human participants with no ocular pathology across a range of ages and refractive errors. We show that there is no effect of age or refractive error on ipRGC inputs to the human pupil control pathway. The stability of the ipRGC mediated pupil response across the human lifespan provides a functional correlate of their robustness observed during ageing in rodent models.

No MeSH data available.


Related in: MedlinePlus