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Effect of Age and Refractive Error on the Melanopsin Mediated Post-Illumination Pupil Response (PIPR).

Adhikari P, Pearson CA, Anderson AM, Zele AJ, Feigl B - Sci Rep (2015)

Bottom Line: Melanopsin containing intrinsically photosensitive Retinal Ganglion cells (ipRGCs) mediate the pupil light reflex (PLR) during light onset and at light offset (the post-illumination pupil response, PIPR).Recent evidence shows that the PLR and PIPR can provide non-invasive, objective markers of age-related retinal and optic nerve disease; however there is no consensus on the effects of healthy ageing or refractive error on the ipRGC mediated pupil function.Here we isolated melanopsin contributions to the pupil control pathway in 59 human participants with no ocular pathology across a range of ages and refractive errors.

View Article: PubMed Central - PubMed

Affiliation: Medical Retina and Visual Science Laboratories, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Brisbane QLD 4059, Australia.

ABSTRACT
Melanopsin containing intrinsically photosensitive Retinal Ganglion cells (ipRGCs) mediate the pupil light reflex (PLR) during light onset and at light offset (the post-illumination pupil response, PIPR). Recent evidence shows that the PLR and PIPR can provide non-invasive, objective markers of age-related retinal and optic nerve disease; however there is no consensus on the effects of healthy ageing or refractive error on the ipRGC mediated pupil function. Here we isolated melanopsin contributions to the pupil control pathway in 59 human participants with no ocular pathology across a range of ages and refractive errors. We show that there is no effect of age or refractive error on ipRGC inputs to the human pupil control pathway. The stability of the ipRGC mediated pupil response across the human lifespan provides a functional correlate of their robustness observed during ageing in rodent models.

No MeSH data available.


Related in: MedlinePlus

Stimulus protocol for pupillometry.Red stimuli (red rectangles) and blue stimuli (blue rectangles) were alternated. The double slash before each 5 s pre-stimulus duration indicates a 10 s interval. Measurements were repeated twice; a two minutes break was given between the repeats of this sequence. PRE = pre-stimulus duration; PIPR = post-illumination pupil response.
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f1: Stimulus protocol for pupillometry.Red stimuli (red rectangles) and blue stimuli (blue rectangles) were alternated. The double slash before each 5 s pre-stimulus duration indicates a 10 s interval. Measurements were repeated twice; a two minutes break was given between the repeats of this sequence. PRE = pre-stimulus duration; PIPR = post-illumination pupil response.

Mentions: All measurements were preceded by 10 minutes dark adaptation in our laboratory (<6 lux). Testing with 1 s light pulses always preceded the 10 s pulses (Fig. 1). Red and blue stimuli were alternated in all sessions to control for the effect of melanopsin bistability31. The order of stimulus presentation was therefore: 1 s red, 1 s blue, 10 s red, and 10 s blue. With every stimulus, the baseline pupil diameter was measured in the dark during 5 s of fixation before the onset of light pulse (1 s or 10 s) and the PIPR was recorded for 40 s after stimulus offset. The PLR was measured under two conditions: 1) Undilated; one eye was stimulated and both the direct and consensual PLR were recorded and 2) dilated; the stimulated eye was dilated with 1% Tropicamide (Minims, Chauvin Pharmaceuticals Ltd., England) and the consensual PLR in the fellow eye was recorded. The dilated pupil was randomly selected. Measurements were repeated two times. Participants were tested between 10 AM and 5 PM to minimise the effect of circadian variation on the PIPR amplitude3233.


Effect of Age and Refractive Error on the Melanopsin Mediated Post-Illumination Pupil Response (PIPR).

Adhikari P, Pearson CA, Anderson AM, Zele AJ, Feigl B - Sci Rep (2015)

Stimulus protocol for pupillometry.Red stimuli (red rectangles) and blue stimuli (blue rectangles) were alternated. The double slash before each 5 s pre-stimulus duration indicates a 10 s interval. Measurements were repeated twice; a two minutes break was given between the repeats of this sequence. PRE = pre-stimulus duration; PIPR = post-illumination pupil response.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664956&req=5

f1: Stimulus protocol for pupillometry.Red stimuli (red rectangles) and blue stimuli (blue rectangles) were alternated. The double slash before each 5 s pre-stimulus duration indicates a 10 s interval. Measurements were repeated twice; a two minutes break was given between the repeats of this sequence. PRE = pre-stimulus duration; PIPR = post-illumination pupil response.
Mentions: All measurements were preceded by 10 minutes dark adaptation in our laboratory (<6 lux). Testing with 1 s light pulses always preceded the 10 s pulses (Fig. 1). Red and blue stimuli were alternated in all sessions to control for the effect of melanopsin bistability31. The order of stimulus presentation was therefore: 1 s red, 1 s blue, 10 s red, and 10 s blue. With every stimulus, the baseline pupil diameter was measured in the dark during 5 s of fixation before the onset of light pulse (1 s or 10 s) and the PIPR was recorded for 40 s after stimulus offset. The PLR was measured under two conditions: 1) Undilated; one eye was stimulated and both the direct and consensual PLR were recorded and 2) dilated; the stimulated eye was dilated with 1% Tropicamide (Minims, Chauvin Pharmaceuticals Ltd., England) and the consensual PLR in the fellow eye was recorded. The dilated pupil was randomly selected. Measurements were repeated two times. Participants were tested between 10 AM and 5 PM to minimise the effect of circadian variation on the PIPR amplitude3233.

Bottom Line: Melanopsin containing intrinsically photosensitive Retinal Ganglion cells (ipRGCs) mediate the pupil light reflex (PLR) during light onset and at light offset (the post-illumination pupil response, PIPR).Recent evidence shows that the PLR and PIPR can provide non-invasive, objective markers of age-related retinal and optic nerve disease; however there is no consensus on the effects of healthy ageing or refractive error on the ipRGC mediated pupil function.Here we isolated melanopsin contributions to the pupil control pathway in 59 human participants with no ocular pathology across a range of ages and refractive errors.

View Article: PubMed Central - PubMed

Affiliation: Medical Retina and Visual Science Laboratories, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Brisbane QLD 4059, Australia.

ABSTRACT
Melanopsin containing intrinsically photosensitive Retinal Ganglion cells (ipRGCs) mediate the pupil light reflex (PLR) during light onset and at light offset (the post-illumination pupil response, PIPR). Recent evidence shows that the PLR and PIPR can provide non-invasive, objective markers of age-related retinal and optic nerve disease; however there is no consensus on the effects of healthy ageing or refractive error on the ipRGC mediated pupil function. Here we isolated melanopsin contributions to the pupil control pathway in 59 human participants with no ocular pathology across a range of ages and refractive errors. We show that there is no effect of age or refractive error on ipRGC inputs to the human pupil control pathway. The stability of the ipRGC mediated pupil response across the human lifespan provides a functional correlate of their robustness observed during ageing in rodent models.

No MeSH data available.


Related in: MedlinePlus