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Suppression on plant-parasitic nematodes using a soil fumigation strategy based on ammonium bicarbonate and its effects on the nematode community.

Su L, Ruan Y, Yang X, Wang K, Li R, Shen Q - Sci Rep (2015)

Bottom Line: Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount.Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores.This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Lab for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, 210095, Nanjing, China.

ABSTRACT
Banana production is severely hindered by plant-parasitic nematodes in acidic, sandy soil. This study investigated the possibility of applying a novel fumigation agent based on ammonium bicarbonate as a strategy for controlling plant-parasitic nematodes under sealed conditions. Moreover, its effects on the nematode community in pot and field experiments were also measured using morphology and feeding-habit based classification and the PCR-DGGE method. Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount. The nematode community was altered by the different fumigants, and LAB showed an excellent plant-parasitic nematicidal ability, especially for Meloidogyne and Rotylenchulus, as revealed by morphology and feeding-habit based classification, and for Meloidogyne, as revealed by the PCR-DGGE method. Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores. This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

No MeSH data available.


Related in: MedlinePlus

Nematode community associated with CK and L controls, AB, LAB and AH treatments after fumigation of the pot experiment, as determined by PCR-DGGE.The cluster diagram of the PCR-DGGE fingerprints from the different soil samples is based on the 18S rRNA nematode gene. Profiles were analyzed using the Dice coefficient and a UPGMA algorithm. Bands marked were excised, cloned and sequenced.
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f4: Nematode community associated with CK and L controls, AB, LAB and AH treatments after fumigation of the pot experiment, as determined by PCR-DGGE.The cluster diagram of the PCR-DGGE fingerprints from the different soil samples is based on the 18S rRNA nematode gene. Profiles were analyzed using the Dice coefficient and a UPGMA algorithm. Bands marked were excised, cloned and sequenced.

Mentions: The DGGE fingerprint analyses of the soil samples after fumigation are shown in Fig. 4. Based on a UPGMA method analysis, the DGGE lanes grouped into 3 clusters were distinctive. The three-replicate cluster for the soil treated by LAB was similar to that from the AB treatment and different from the other clusters (Fig. 4).


Suppression on plant-parasitic nematodes using a soil fumigation strategy based on ammonium bicarbonate and its effects on the nematode community.

Su L, Ruan Y, Yang X, Wang K, Li R, Shen Q - Sci Rep (2015)

Nematode community associated with CK and L controls, AB, LAB and AH treatments after fumigation of the pot experiment, as determined by PCR-DGGE.The cluster diagram of the PCR-DGGE fingerprints from the different soil samples is based on the 18S rRNA nematode gene. Profiles were analyzed using the Dice coefficient and a UPGMA algorithm. Bands marked were excised, cloned and sequenced.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664931&req=5

f4: Nematode community associated with CK and L controls, AB, LAB and AH treatments after fumigation of the pot experiment, as determined by PCR-DGGE.The cluster diagram of the PCR-DGGE fingerprints from the different soil samples is based on the 18S rRNA nematode gene. Profiles were analyzed using the Dice coefficient and a UPGMA algorithm. Bands marked were excised, cloned and sequenced.
Mentions: The DGGE fingerprint analyses of the soil samples after fumigation are shown in Fig. 4. Based on a UPGMA method analysis, the DGGE lanes grouped into 3 clusters were distinctive. The three-replicate cluster for the soil treated by LAB was similar to that from the AB treatment and different from the other clusters (Fig. 4).

Bottom Line: Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount.Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores.This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Lab for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, 210095, Nanjing, China.

ABSTRACT
Banana production is severely hindered by plant-parasitic nematodes in acidic, sandy soil. This study investigated the possibility of applying a novel fumigation agent based on ammonium bicarbonate as a strategy for controlling plant-parasitic nematodes under sealed conditions. Moreover, its effects on the nematode community in pot and field experiments were also measured using morphology and feeding-habit based classification and the PCR-DGGE method. Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount. The nematode community was altered by the different fumigants, and LAB showed an excellent plant-parasitic nematicidal ability, especially for Meloidogyne and Rotylenchulus, as revealed by morphology and feeding-habit based classification, and for Meloidogyne, as revealed by the PCR-DGGE method. Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores. This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

No MeSH data available.


Related in: MedlinePlus