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Suppression on plant-parasitic nematodes using a soil fumigation strategy based on ammonium bicarbonate and its effects on the nematode community.

Su L, Ruan Y, Yang X, Wang K, Li R, Shen Q - Sci Rep (2015)

Bottom Line: Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount.Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores.This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Lab for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, 210095, Nanjing, China.

ABSTRACT
Banana production is severely hindered by plant-parasitic nematodes in acidic, sandy soil. This study investigated the possibility of applying a novel fumigation agent based on ammonium bicarbonate as a strategy for controlling plant-parasitic nematodes under sealed conditions. Moreover, its effects on the nematode community in pot and field experiments were also measured using morphology and feeding-habit based classification and the PCR-DGGE method. Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount. The nematode community was altered by the different fumigants, and LAB showed an excellent plant-parasitic nematicidal ability, especially for Meloidogyne and Rotylenchulus, as revealed by morphology and feeding-habit based classification, and for Meloidogyne, as revealed by the PCR-DGGE method. Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores. This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

No MeSH data available.


Related in: MedlinePlus

Effects of 20- and 50- fold dilutions of LAB (0.857 g of lime and 0.428 g of AB, d.w.), AB (0.428 g of AB, d.w.), CC (0.257 g of calcium cyanamide, d.w.) and AH (0.139 g of ammonium hydroxide, d.w.) on the corrected mortality of nematodes.The error line means standard deviation of the mean. Bars with different letters indicate significant differences among the treatments, as defined by Duncan’s test (P < 0.05).
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f2: Effects of 20- and 50- fold dilutions of LAB (0.857 g of lime and 0.428 g of AB, d.w.), AB (0.428 g of AB, d.w.), CC (0.257 g of calcium cyanamide, d.w.) and AH (0.139 g of ammonium hydroxide, d.w.) on the corrected mortality of nematodes.The error line means standard deviation of the mean. Bars with different letters indicate significant differences among the treatments, as defined by Duncan’s test (P < 0.05).

Mentions: The nematicidal effect of aqueous solutions of LAB, AB, CC and AH evaluated in a Petri dish experiment are shown in Fig. 2. Both diluents of LAB almost completely killed the nematodes. In the 20-fold diluent experiment, compared with the control (Petri dish with no additives) with mortality of 12%, the corrected mortality of the nematodes for LAB, AB, CC and AH treatments were 98%, 95%, 54% and 93%, respectively. In the 50-fold diluent experiment, the values were 98%, 85%, 36% and 86%, respectively. These Petri dish experiment results indicate that LAB is the most efficient nematicidal agent.


Suppression on plant-parasitic nematodes using a soil fumigation strategy based on ammonium bicarbonate and its effects on the nematode community.

Su L, Ruan Y, Yang X, Wang K, Li R, Shen Q - Sci Rep (2015)

Effects of 20- and 50- fold dilutions of LAB (0.857 g of lime and 0.428 g of AB, d.w.), AB (0.428 g of AB, d.w.), CC (0.257 g of calcium cyanamide, d.w.) and AH (0.139 g of ammonium hydroxide, d.w.) on the corrected mortality of nematodes.The error line means standard deviation of the mean. Bars with different letters indicate significant differences among the treatments, as defined by Duncan’s test (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664931&req=5

f2: Effects of 20- and 50- fold dilutions of LAB (0.857 g of lime and 0.428 g of AB, d.w.), AB (0.428 g of AB, d.w.), CC (0.257 g of calcium cyanamide, d.w.) and AH (0.139 g of ammonium hydroxide, d.w.) on the corrected mortality of nematodes.The error line means standard deviation of the mean. Bars with different letters indicate significant differences among the treatments, as defined by Duncan’s test (P < 0.05).
Mentions: The nematicidal effect of aqueous solutions of LAB, AB, CC and AH evaluated in a Petri dish experiment are shown in Fig. 2. Both diluents of LAB almost completely killed the nematodes. In the 20-fold diluent experiment, compared with the control (Petri dish with no additives) with mortality of 12%, the corrected mortality of the nematodes for LAB, AB, CC and AH treatments were 98%, 95%, 54% and 93%, respectively. In the 50-fold diluent experiment, the values were 98%, 85%, 36% and 86%, respectively. These Petri dish experiment results indicate that LAB is the most efficient nematicidal agent.

Bottom Line: Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount.Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores.This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Lab for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, 210095, Nanjing, China.

ABSTRACT
Banana production is severely hindered by plant-parasitic nematodes in acidic, sandy soil. This study investigated the possibility of applying a novel fumigation agent based on ammonium bicarbonate as a strategy for controlling plant-parasitic nematodes under sealed conditions. Moreover, its effects on the nematode community in pot and field experiments were also measured using morphology and feeding-habit based classification and the PCR-DGGE method. Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount. The nematode community was altered by the different fumigants, and LAB showed an excellent plant-parasitic nematicidal ability, especially for Meloidogyne and Rotylenchulus, as revealed by morphology and feeding-habit based classification, and for Meloidogyne, as revealed by the PCR-DGGE method. Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores. This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

No MeSH data available.


Related in: MedlinePlus