Limits...
Suppression on plant-parasitic nematodes using a soil fumigation strategy based on ammonium bicarbonate and its effects on the nematode community.

Su L, Ruan Y, Yang X, Wang K, Li R, Shen Q - Sci Rep (2015)

Bottom Line: Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount.Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores.This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Lab for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, 210095, Nanjing, China.

ABSTRACT
Banana production is severely hindered by plant-parasitic nematodes in acidic, sandy soil. This study investigated the possibility of applying a novel fumigation agent based on ammonium bicarbonate as a strategy for controlling plant-parasitic nematodes under sealed conditions. Moreover, its effects on the nematode community in pot and field experiments were also measured using morphology and feeding-habit based classification and the PCR-DGGE method. Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount. The nematode community was altered by the different fumigants, and LAB showed an excellent plant-parasitic nematicidal ability, especially for Meloidogyne and Rotylenchulus, as revealed by morphology and feeding-habit based classification, and for Meloidogyne, as revealed by the PCR-DGGE method. Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores. This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

No MeSH data available.


Related in: MedlinePlus

Effects of different concentrations of L, AB and LAB on the number of nematodes (a); effects of water content (b) and temperature (c) on the suppressed nematodes ability of soils treated with 0.857 g of L and 0.428 g of AB.The error line means standard deviation of the mean. Bars with different letters indicate significant differences of the different concentrations for each treatments (a) and among the treatments (b,c) as defined by Duncan’s test (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664931&req=5

f1: Effects of different concentrations of L, AB and LAB on the number of nematodes (a); effects of water content (b) and temperature (c) on the suppressed nematodes ability of soils treated with 0.857 g of L and 0.428 g of AB.The error line means standard deviation of the mean. Bars with different letters indicate significant differences of the different concentrations for each treatments (a) and among the treatments (b,c) as defined by Duncan’s test (P < 0.05).

Mentions: The effects of different concentrations of L, AB and LAB on nematode numbers are shown in Fig. 1a. For all the results, the maximum population was found in the CK (pot soils with no additives), AB1 and L4. The total number of nematodes showed a continuous decrease in the AB and LAB treatments and an irregular fluctuation in the L treatment. The minimum value was observed after treatment with AB and LAB in the highest amount added, and less than 300 individuals per 100 g d.w. soil were identified when the amount added was more than 0.857 g of L and 0.428 g of AB. Thus, this concentration (LAB4) was selected for further study. Compared with the AB treatment, the LAB treatment improved the effects for reducing the nematodes numbers by 51.4%, 70.4%, 45.0%, 55.7% and 83.7% with the increase of additional concentrations, respectively.


Suppression on plant-parasitic nematodes using a soil fumigation strategy based on ammonium bicarbonate and its effects on the nematode community.

Su L, Ruan Y, Yang X, Wang K, Li R, Shen Q - Sci Rep (2015)

Effects of different concentrations of L, AB and LAB on the number of nematodes (a); effects of water content (b) and temperature (c) on the suppressed nematodes ability of soils treated with 0.857 g of L and 0.428 g of AB.The error line means standard deviation of the mean. Bars with different letters indicate significant differences of the different concentrations for each treatments (a) and among the treatments (b,c) as defined by Duncan’s test (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664931&req=5

f1: Effects of different concentrations of L, AB and LAB on the number of nematodes (a); effects of water content (b) and temperature (c) on the suppressed nematodes ability of soils treated with 0.857 g of L and 0.428 g of AB.The error line means standard deviation of the mean. Bars with different letters indicate significant differences of the different concentrations for each treatments (a) and among the treatments (b,c) as defined by Duncan’s test (P < 0.05).
Mentions: The effects of different concentrations of L, AB and LAB on nematode numbers are shown in Fig. 1a. For all the results, the maximum population was found in the CK (pot soils with no additives), AB1 and L4. The total number of nematodes showed a continuous decrease in the AB and LAB treatments and an irregular fluctuation in the L treatment. The minimum value was observed after treatment with AB and LAB in the highest amount added, and less than 300 individuals per 100 g d.w. soil were identified when the amount added was more than 0.857 g of L and 0.428 g of AB. Thus, this concentration (LAB4) was selected for further study. Compared with the AB treatment, the LAB treatment improved the effects for reducing the nematodes numbers by 51.4%, 70.4%, 45.0%, 55.7% and 83.7% with the increase of additional concentrations, respectively.

Bottom Line: Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount.Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores.This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Lab for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, 210095, Nanjing, China.

ABSTRACT
Banana production is severely hindered by plant-parasitic nematodes in acidic, sandy soil. This study investigated the possibility of applying a novel fumigation agent based on ammonium bicarbonate as a strategy for controlling plant-parasitic nematodes under sealed conditions. Moreover, its effects on the nematode community in pot and field experiments were also measured using morphology and feeding-habit based classification and the PCR-DGGE method. Results showed that a mixture (LAB) of lime (L) and ammonium bicarbonate (AB) in suitable additive amounts (0.857 g kg(-1) of L and 0.428 g kg(-1) of AB) showed stronger nematicidal ability than did the use of AB alone or the use of ammonium hydroxide (AH) and calcium cyanamide (CC) with an equal nitrogen amount. The nematode community was altered by the different fumigants, and LAB showed an excellent plant-parasitic nematicidal ability, especially for Meloidogyne and Rotylenchulus, as revealed by morphology and feeding-habit based classification, and for Meloidogyne, as revealed by the PCR-DGGE method. Fungivores and omnivore-predators were more sensitive to the direct effects of the chemicals than bacterivores. This study explored a novel fumigation agent for controlling plant-parasitic nematodes based on LAB and provides a potential strategy to ensure the worldwide development of the banana industry.

No MeSH data available.


Related in: MedlinePlus