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Improved In vivo Assessment of Pulmonary Fibrosis in Mice using X-Ray Dark-Field Radiography.

Yaroshenko A, Hellbach K, Yildirim AÖ, Conlon TM, Fernandez IE, Bech M, Velroyen A, Meinel FG, Auweter S, Reiser M, Eickelberg O, Pfeiffer F - Sci Rep (2015)

Bottom Line: This imaging method is based on the detection of small-angle x-ray scattering that occurs at the air-tissue interfaces in the lung.The presented radiography method is significantly more sensitive in detecting morphological changes compared with conventional x-ray imaging, and exhibits a significantly lower radiation dose than conventional x-ray CT.As a result of the improved imaging sensitivity, this new imaging modality could be used in future to reduce the number of animals required for pulmonary research studies.

View Article: PubMed Central - PubMed

Affiliation: Lehrstuhl für Biomedizinische Physik, Physik-Department &Institut für Medizintechnik, Technische Universität München, Garching, Germany.

ABSTRACT
Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with a median life expectancy of 4-5 years after initial diagnosis. Early diagnosis and accurate monitoring of IPF are limited by a lack of sensitive imaging techniques that are able to visualize early fibrotic changes at the epithelial-mesenchymal interface. Here, we report a new x-ray imaging approach that directly visualizes the air-tissue interfaces in mice in vivo. This imaging method is based on the detection of small-angle x-ray scattering that occurs at the air-tissue interfaces in the lung. Small-angle scattering is detected with a Talbot-Lau interferometer, which provides the so-called x-ray dark-field signal. Using this imaging modality, we demonstrate-for the first time-the quantification of early pathogenic changes and their correlation with histological changes, as assessed by stereological morphometry. The presented radiography method is significantly more sensitive in detecting morphological changes compared with conventional x-ray imaging, and exhibits a significantly lower radiation dose than conventional x-ray CT. As a result of the improved imaging sensitivity, this new imaging modality could be used in future to reduce the number of animals required for pulmonary research studies.

No MeSH data available.


Related in: MedlinePlus

Histological slice (and a magnified section) of a control lung (A) and a lung treated with bleomycin (B).The quantified lung tissue percentage for all the animals in the control and the bleomycin groups is presented in (C). (D,E) reveal the tissue elastance and dynamic compliance for the two animal groups obtained from the pulmonary function tests.
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f1: Histological slice (and a magnified section) of a control lung (A) and a lung treated with bleomycin (B).The quantified lung tissue percentage for all the animals in the control and the bleomycin groups is presented in (C). (D,E) reveal the tissue elastance and dynamic compliance for the two animal groups obtained from the pulmonary function tests.

Mentions: Histopathology of the harvested lungs revealed a clear difference between the control and the bleomycin group. Figure 1 shows a typical histological slice (and a magnified section) of a healthy lung (A) and a lung showing fibrotic changes (B). The control lung is clearly constituted by a healthy, dense alveolar network. By contrast, in case of the animal treated with bleomycin the distal airspaces are filled with extracellular matrix components, with severe consequences for the functionality of the lung. Thus, typical morphological changes associated with pulmonary fibrosis were observed on histological slices, so that it can be concluded that any changes observed on the x-ray images are associated with the presence of fibrosis.


Improved In vivo Assessment of Pulmonary Fibrosis in Mice using X-Ray Dark-Field Radiography.

Yaroshenko A, Hellbach K, Yildirim AÖ, Conlon TM, Fernandez IE, Bech M, Velroyen A, Meinel FG, Auweter S, Reiser M, Eickelberg O, Pfeiffer F - Sci Rep (2015)

Histological slice (and a magnified section) of a control lung (A) and a lung treated with bleomycin (B).The quantified lung tissue percentage for all the animals in the control and the bleomycin groups is presented in (C). (D,E) reveal the tissue elastance and dynamic compliance for the two animal groups obtained from the pulmonary function tests.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664921&req=5

f1: Histological slice (and a magnified section) of a control lung (A) and a lung treated with bleomycin (B).The quantified lung tissue percentage for all the animals in the control and the bleomycin groups is presented in (C). (D,E) reveal the tissue elastance and dynamic compliance for the two animal groups obtained from the pulmonary function tests.
Mentions: Histopathology of the harvested lungs revealed a clear difference between the control and the bleomycin group. Figure 1 shows a typical histological slice (and a magnified section) of a healthy lung (A) and a lung showing fibrotic changes (B). The control lung is clearly constituted by a healthy, dense alveolar network. By contrast, in case of the animal treated with bleomycin the distal airspaces are filled with extracellular matrix components, with severe consequences for the functionality of the lung. Thus, typical morphological changes associated with pulmonary fibrosis were observed on histological slices, so that it can be concluded that any changes observed on the x-ray images are associated with the presence of fibrosis.

Bottom Line: This imaging method is based on the detection of small-angle x-ray scattering that occurs at the air-tissue interfaces in the lung.The presented radiography method is significantly more sensitive in detecting morphological changes compared with conventional x-ray imaging, and exhibits a significantly lower radiation dose than conventional x-ray CT.As a result of the improved imaging sensitivity, this new imaging modality could be used in future to reduce the number of animals required for pulmonary research studies.

View Article: PubMed Central - PubMed

Affiliation: Lehrstuhl für Biomedizinische Physik, Physik-Department &Institut für Medizintechnik, Technische Universität München, Garching, Germany.

ABSTRACT
Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease with a median life expectancy of 4-5 years after initial diagnosis. Early diagnosis and accurate monitoring of IPF are limited by a lack of sensitive imaging techniques that are able to visualize early fibrotic changes at the epithelial-mesenchymal interface. Here, we report a new x-ray imaging approach that directly visualizes the air-tissue interfaces in mice in vivo. This imaging method is based on the detection of small-angle x-ray scattering that occurs at the air-tissue interfaces in the lung. Small-angle scattering is detected with a Talbot-Lau interferometer, which provides the so-called x-ray dark-field signal. Using this imaging modality, we demonstrate-for the first time-the quantification of early pathogenic changes and their correlation with histological changes, as assessed by stereological morphometry. The presented radiography method is significantly more sensitive in detecting morphological changes compared with conventional x-ray imaging, and exhibits a significantly lower radiation dose than conventional x-ray CT. As a result of the improved imaging sensitivity, this new imaging modality could be used in future to reduce the number of animals required for pulmonary research studies.

No MeSH data available.


Related in: MedlinePlus