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Influence of age, gender, and race on nitric oxide release over acupuncture points-meridians.

Ma SX, Lee PC, Jiang I, Ma E, Hu JS, Li XY - Sci Rep (2015)

Bottom Line: In elder ages compared to adults, NO levels over the ventral forearm were significantly decreased over LU on radial regions but not altered over PC on medial regions.These results suggest a selective reduction of NO release over LU meridian with aging, which is consistent with a progressive decline in lung function and increase in chronic respiratory disease in elder ages.Both of them support somatic-organ connections in the meridian system associated with potential pathophysiological changes with aging.

View Article: PubMed Central - PubMed

Affiliation: LA BioMed Research Institute at Harbor-UCLA Medical Center and Department of Obstetrics and Gynecology, David Geffen School of Medicine at UCLA and Harbor-UCLA Medical Center, Torrance, CA 90502.

ABSTRACT
This study examined the influence of age, gender and race on nitric oxide (NO) release over acupuncture points, meridian without acupoint, and non-meridian regions of the Pericardium (PC) and Bladder (BL) meridian as well as aging on LU meridian in 61 healthy subjects. Biocapture tubes were attached to the skin surface, and total nitrite and nitrate was biocaptured and quantified using chemiluminescence. In elder ages compared to adults, NO levels over the ventral forearm were significantly decreased over LU on radial regions but not altered over PC on medial regions. Conversely, NO content was elevated over BL regions only in overweight/obesity of elder ages. NO levels over PC regions were marginally elevated in overweight/obese males compared to females but did not alter between races. These results suggest a selective reduction of NO release over LU meridian with aging, which is consistent with a progressive decline in lung function and increase in chronic respiratory disease in elder ages. Increased NO levels along the BL meridian in older obese subjects may reflect a modified NO level along somatic-bladder pathway for counteracting bladder dysfunctions with aging. Both of them support somatic-organ connections in the meridian system associated with potential pathophysiological changes with aging.

No MeSH data available.


Related in: MedlinePlus

Quantification of nitric oxide (NO) metabolites over skin acupoints/meridians in male compared to female subjects.(a) Concentrations of total nitrite plus nitrate (NOx−) were compared between male and female subjects over the regions along the Pericardium (PC) meridian (n = 18 and 28) and Bladder (BL) meridian (n = 14 and 23). (b) NOx− concentrations over PC regions between male and female subjects were compared in normal weight (n = 8 and 16) and overweight/obesity (n = 10 and 12). NOx− concentrations (nmol/cm2) were obtained over acupoints, meridian lines without acupoint (MWOP), and non-meridian control region (NMCR). Each bar represents the mean values and vertical bars represent S.E.M. *p = 0.05, compared to male; #p = 0.08, compared to male.
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f3: Quantification of nitric oxide (NO) metabolites over skin acupoints/meridians in male compared to female subjects.(a) Concentrations of total nitrite plus nitrate (NOx−) were compared between male and female subjects over the regions along the Pericardium (PC) meridian (n = 18 and 28) and Bladder (BL) meridian (n = 14 and 23). (b) NOx− concentrations over PC regions between male and female subjects were compared in normal weight (n = 8 and 16) and overweight/obesity (n = 10 and 12). NOx− concentrations (nmol/cm2) were obtained over acupoints, meridian lines without acupoint (MWOP), and non-meridian control region (NMCR). Each bar represents the mean values and vertical bars represent S.E.M. *p = 0.05, compared to male; #p = 0.08, compared to male.

Mentions: To determine the influence of gender on NO releases, NOx− concentrations over PC and BL regions were compared between male and female subjects in 55 healthy volunteers, as shown in Fig. 3a. A two-way ANOVA revealed that there is no significant differences in NOx− concentrations in males compared to females over PC and BL regions in normal weight plus overweight/obese subjects, but there is a marginally significant interaction between gender and BMI on NOx− concentrations over PC regions only (F1,41 = 3.9, P = 0.06). In overweight/obese males, NOx− concentration was marginally increased over PC regions compared to overweight/obese females (F1,20 = 2.6, P = 0.12), as shown in Fig. 3b. NOx− levels over PC MWOP and NMCR in overweight/obese subjects were marginally and significantly elevated in males compared to females (P = 0.08, P = 0.05, respectively). NOx− concentrations (nmol/cm2 Mean ± SE) over PC NMCR, MWOP, and acupoints in adult females of normal weight subgroup (BMI: 21.1 ± 0.66, n = 10) were 0.29 ± 0.05, 0.32 ± 0.05, and 0.34 ± 0.05, which are similar with 0.31 ± 0.06, 0.27 ± 0.04, and 0.33 ± 0.06 in adult female of overweight/obese subjects (BMI: 29.5 ± 1.43, n = 7). In adult males of normal weight versus overweight/obesity subgroups, the values of NO concentrations over PC acupoints, MWOP, and NMCR are higher in overweight/obesity subgroup than those in normal weight subgroup, but fell short of statistical significance (n = 4 in each subgroup). However, there were no detectable changes in NO contents over BL regions in males compared to females both in normal weight and overweight/obese groups.


Influence of age, gender, and race on nitric oxide release over acupuncture points-meridians.

Ma SX, Lee PC, Jiang I, Ma E, Hu JS, Li XY - Sci Rep (2015)

Quantification of nitric oxide (NO) metabolites over skin acupoints/meridians in male compared to female subjects.(a) Concentrations of total nitrite plus nitrate (NOx−) were compared between male and female subjects over the regions along the Pericardium (PC) meridian (n = 18 and 28) and Bladder (BL) meridian (n = 14 and 23). (b) NOx− concentrations over PC regions between male and female subjects were compared in normal weight (n = 8 and 16) and overweight/obesity (n = 10 and 12). NOx− concentrations (nmol/cm2) were obtained over acupoints, meridian lines without acupoint (MWOP), and non-meridian control region (NMCR). Each bar represents the mean values and vertical bars represent S.E.M. *p = 0.05, compared to male; #p = 0.08, compared to male.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4664911&req=5

f3: Quantification of nitric oxide (NO) metabolites over skin acupoints/meridians in male compared to female subjects.(a) Concentrations of total nitrite plus nitrate (NOx−) were compared between male and female subjects over the regions along the Pericardium (PC) meridian (n = 18 and 28) and Bladder (BL) meridian (n = 14 and 23). (b) NOx− concentrations over PC regions between male and female subjects were compared in normal weight (n = 8 and 16) and overweight/obesity (n = 10 and 12). NOx− concentrations (nmol/cm2) were obtained over acupoints, meridian lines without acupoint (MWOP), and non-meridian control region (NMCR). Each bar represents the mean values and vertical bars represent S.E.M. *p = 0.05, compared to male; #p = 0.08, compared to male.
Mentions: To determine the influence of gender on NO releases, NOx− concentrations over PC and BL regions were compared between male and female subjects in 55 healthy volunteers, as shown in Fig. 3a. A two-way ANOVA revealed that there is no significant differences in NOx− concentrations in males compared to females over PC and BL regions in normal weight plus overweight/obese subjects, but there is a marginally significant interaction between gender and BMI on NOx− concentrations over PC regions only (F1,41 = 3.9, P = 0.06). In overweight/obese males, NOx− concentration was marginally increased over PC regions compared to overweight/obese females (F1,20 = 2.6, P = 0.12), as shown in Fig. 3b. NOx− levels over PC MWOP and NMCR in overweight/obese subjects were marginally and significantly elevated in males compared to females (P = 0.08, P = 0.05, respectively). NOx− concentrations (nmol/cm2 Mean ± SE) over PC NMCR, MWOP, and acupoints in adult females of normal weight subgroup (BMI: 21.1 ± 0.66, n = 10) were 0.29 ± 0.05, 0.32 ± 0.05, and 0.34 ± 0.05, which are similar with 0.31 ± 0.06, 0.27 ± 0.04, and 0.33 ± 0.06 in adult female of overweight/obese subjects (BMI: 29.5 ± 1.43, n = 7). In adult males of normal weight versus overweight/obesity subgroups, the values of NO concentrations over PC acupoints, MWOP, and NMCR are higher in overweight/obesity subgroup than those in normal weight subgroup, but fell short of statistical significance (n = 4 in each subgroup). However, there were no detectable changes in NO contents over BL regions in males compared to females both in normal weight and overweight/obese groups.

Bottom Line: In elder ages compared to adults, NO levels over the ventral forearm were significantly decreased over LU on radial regions but not altered over PC on medial regions.These results suggest a selective reduction of NO release over LU meridian with aging, which is consistent with a progressive decline in lung function and increase in chronic respiratory disease in elder ages.Both of them support somatic-organ connections in the meridian system associated with potential pathophysiological changes with aging.

View Article: PubMed Central - PubMed

Affiliation: LA BioMed Research Institute at Harbor-UCLA Medical Center and Department of Obstetrics and Gynecology, David Geffen School of Medicine at UCLA and Harbor-UCLA Medical Center, Torrance, CA 90502.

ABSTRACT
This study examined the influence of age, gender and race on nitric oxide (NO) release over acupuncture points, meridian without acupoint, and non-meridian regions of the Pericardium (PC) and Bladder (BL) meridian as well as aging on LU meridian in 61 healthy subjects. Biocapture tubes were attached to the skin surface, and total nitrite and nitrate was biocaptured and quantified using chemiluminescence. In elder ages compared to adults, NO levels over the ventral forearm were significantly decreased over LU on radial regions but not altered over PC on medial regions. Conversely, NO content was elevated over BL regions only in overweight/obesity of elder ages. NO levels over PC regions were marginally elevated in overweight/obese males compared to females but did not alter between races. These results suggest a selective reduction of NO release over LU meridian with aging, which is consistent with a progressive decline in lung function and increase in chronic respiratory disease in elder ages. Increased NO levels along the BL meridian in older obese subjects may reflect a modified NO level along somatic-bladder pathway for counteracting bladder dysfunctions with aging. Both of them support somatic-organ connections in the meridian system associated with potential pathophysiological changes with aging.

No MeSH data available.


Related in: MedlinePlus