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Targeting TRPV1 for Body Weight Control using TRPV1(-/-) Mice and Electroacupuncture.

Choowanthanapakorn M, Lu KW, Yang J, Hsieh CL, Lin YW - Sci Rep (2015)

Bottom Line: The results demonstrated that body weight increased with time in the control group (108.19 ± 1.31%, n = 7).EA did not decrease the gain of body weight in TRPV1 knock mice (107.94 ± 0.41% and 107.79 ± 1.04% for TRPV1(-/-) and TRPV1(-/-) with EA, respectively, p > 0.05).The visceral white adipose tissue (WAT) weight was lower in the EA group at 4 weeks after manipulation.

View Article: PubMed Central - PubMed

Affiliation: College of Chinese Medicine, Graduate Institute of Acupuncture Science and International Master Program, China Medical University, Taichung 40402, Taiwan.

ABSTRACT
Obesity is a global social medical problem resulting in morbidity as high as 20-30%. Here we investigated whether the manipulation of TRPV1 can control mice body weight through electroacupuncture (EA). The results demonstrated that body weight increased with time in the control group (108.19 ± 1.31%, n = 7). The increase of mice body weight was significantly less in the EA group (104.41 ± 0.76%, p < 0.05, compared with the control group, n = 7) but not in the sham EA group (109.1 ± 0.63%, p < 0.05, compared with EA group, n = 7). EA did not decrease the gain of body weight in TRPV1 knock mice (107.94 ± 0.41% and 107.79 ± 1.04% for TRPV1(-/-) and TRPV1(-/-) with EA, respectively, p > 0.05). The visceral white adipose tissue (WAT) weight was lower in the EA group at 4 weeks after manipulation. Moreover, the protein levels of TRPV1, pPKA, pPKC, and pERK were increased in the dorsal root ganglion (DRG) and spinal cord (SC) after EA treatment but not in the sham EA and TRPV1(-/-) mice. This study suggests that targeting TRPV1 is beneficial in controlling body weight and TRPV1-associated mechanisms in mice.

No MeSH data available.


Related in: MedlinePlus

Upregulation of TRPV1 and relevant molecules in SC from EA but not sham-EA mice.(A) Western blot staining showed TRPV1-positive band; (B) pPKA-positive band; (C) pPKC-positive band; (D) pERK-positive band; (E) pp38-positive band; (F) pJNK-positive band in Con, EA, sham groups. Con = control; EA = electroacupuncture; sham = sham-EA group.
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f4: Upregulation of TRPV1 and relevant molecules in SC from EA but not sham-EA mice.(A) Western blot staining showed TRPV1-positive band; (B) pPKA-positive band; (C) pPKC-positive band; (D) pERK-positive band; (E) pp38-positive band; (F) pJNK-positive band in Con, EA, sham groups. Con = control; EA = electroacupuncture; sham = sham-EA group.

Mentions: To verify if EA can regulate body weight at central–spinal level, we tested TRPV1 and the related downstream mechanisms using SC sample. TRPV1 was increased after EA treatment (Fig. 4, 115.45 ± 1.43%, p < 0.05, compared to the control group, n = 7) but not in the sham-EA group (Fig. 4, 100.8 ± 2.97%, p < 0.05, compared to the EA group, n = 7). Our results showed that the quantity of pPKA was increased after EA manipulation (Fig. 4, 112.87 ± 2.68%, p < 0.05, compared to the control group, n = 7) but not in the sham-EA group (Fig. 4, 108.17 ± 10.62%, p < 0.05, compared to the control group, n = 7). Similar data were also observed in pPKC in the EA-treated group (Fig. 4, 102.94 ± 5.21% and 96.69 ± 5.3% for EA and sham-EA group, respectively, p < 0.05, n = 7). These results also showed that pERK was drastically increased after EA treatment (Fig. 4, 121.87 ± 4.62%, p < 0.05, compared to the control group, n = 7) but not in the sham control (Fig. 4, 98.88 ± 6.04%, p < 0.05, compared to the EA group, n = 7). The results were not obtained in pp38 (Fig. 4, 101.74 ± 8.59% and 97.79 ± 6.45% for EA and sham-EA group, respectively, p < 0.05, n = 7) and pJNK (Fig. 4, 102.95 ± 6.35% and 102.26 ± 5.27% for EA and sham-EA group, respectively, p < 0.05, n = 7).


Targeting TRPV1 for Body Weight Control using TRPV1(-/-) Mice and Electroacupuncture.

Choowanthanapakorn M, Lu KW, Yang J, Hsieh CL, Lin YW - Sci Rep (2015)

Upregulation of TRPV1 and relevant molecules in SC from EA but not sham-EA mice.(A) Western blot staining showed TRPV1-positive band; (B) pPKA-positive band; (C) pPKC-positive band; (D) pERK-positive band; (E) pp38-positive band; (F) pJNK-positive band in Con, EA, sham groups. Con = control; EA = electroacupuncture; sham = sham-EA group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664894&req=5

f4: Upregulation of TRPV1 and relevant molecules in SC from EA but not sham-EA mice.(A) Western blot staining showed TRPV1-positive band; (B) pPKA-positive band; (C) pPKC-positive band; (D) pERK-positive band; (E) pp38-positive band; (F) pJNK-positive band in Con, EA, sham groups. Con = control; EA = electroacupuncture; sham = sham-EA group.
Mentions: To verify if EA can regulate body weight at central–spinal level, we tested TRPV1 and the related downstream mechanisms using SC sample. TRPV1 was increased after EA treatment (Fig. 4, 115.45 ± 1.43%, p < 0.05, compared to the control group, n = 7) but not in the sham-EA group (Fig. 4, 100.8 ± 2.97%, p < 0.05, compared to the EA group, n = 7). Our results showed that the quantity of pPKA was increased after EA manipulation (Fig. 4, 112.87 ± 2.68%, p < 0.05, compared to the control group, n = 7) but not in the sham-EA group (Fig. 4, 108.17 ± 10.62%, p < 0.05, compared to the control group, n = 7). Similar data were also observed in pPKC in the EA-treated group (Fig. 4, 102.94 ± 5.21% and 96.69 ± 5.3% for EA and sham-EA group, respectively, p < 0.05, n = 7). These results also showed that pERK was drastically increased after EA treatment (Fig. 4, 121.87 ± 4.62%, p < 0.05, compared to the control group, n = 7) but not in the sham control (Fig. 4, 98.88 ± 6.04%, p < 0.05, compared to the EA group, n = 7). The results were not obtained in pp38 (Fig. 4, 101.74 ± 8.59% and 97.79 ± 6.45% for EA and sham-EA group, respectively, p < 0.05, n = 7) and pJNK (Fig. 4, 102.95 ± 6.35% and 102.26 ± 5.27% for EA and sham-EA group, respectively, p < 0.05, n = 7).

Bottom Line: The results demonstrated that body weight increased with time in the control group (108.19 ± 1.31%, n = 7).EA did not decrease the gain of body weight in TRPV1 knock mice (107.94 ± 0.41% and 107.79 ± 1.04% for TRPV1(-/-) and TRPV1(-/-) with EA, respectively, p > 0.05).The visceral white adipose tissue (WAT) weight was lower in the EA group at 4 weeks after manipulation.

View Article: PubMed Central - PubMed

Affiliation: College of Chinese Medicine, Graduate Institute of Acupuncture Science and International Master Program, China Medical University, Taichung 40402, Taiwan.

ABSTRACT
Obesity is a global social medical problem resulting in morbidity as high as 20-30%. Here we investigated whether the manipulation of TRPV1 can control mice body weight through electroacupuncture (EA). The results demonstrated that body weight increased with time in the control group (108.19 ± 1.31%, n = 7). The increase of mice body weight was significantly less in the EA group (104.41 ± 0.76%, p < 0.05, compared with the control group, n = 7) but not in the sham EA group (109.1 ± 0.63%, p < 0.05, compared with EA group, n = 7). EA did not decrease the gain of body weight in TRPV1 knock mice (107.94 ± 0.41% and 107.79 ± 1.04% for TRPV1(-/-) and TRPV1(-/-) with EA, respectively, p > 0.05). The visceral white adipose tissue (WAT) weight was lower in the EA group at 4 weeks after manipulation. Moreover, the protein levels of TRPV1, pPKA, pPKC, and pERK were increased in the dorsal root ganglion (DRG) and spinal cord (SC) after EA treatment but not in the sham EA and TRPV1(-/-) mice. This study suggests that targeting TRPV1 is beneficial in controlling body weight and TRPV1-associated mechanisms in mice.

No MeSH data available.


Related in: MedlinePlus