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Targeting TRPV1 for Body Weight Control using TRPV1(-/-) Mice and Electroacupuncture.

Choowanthanapakorn M, Lu KW, Yang J, Hsieh CL, Lin YW - Sci Rep (2015)

Bottom Line: The results demonstrated that body weight increased with time in the control group (108.19 ± 1.31%, n = 7).EA did not decrease the gain of body weight in TRPV1 knock mice (107.94 ± 0.41% and 107.79 ± 1.04% for TRPV1(-/-) and TRPV1(-/-) with EA, respectively, p > 0.05).The visceral white adipose tissue (WAT) weight was lower in the EA group at 4 weeks after manipulation.

View Article: PubMed Central - PubMed

Affiliation: College of Chinese Medicine, Graduate Institute of Acupuncture Science and International Master Program, China Medical University, Taichung 40402, Taiwan.

ABSTRACT
Obesity is a global social medical problem resulting in morbidity as high as 20-30%. Here we investigated whether the manipulation of TRPV1 can control mice body weight through electroacupuncture (EA). The results demonstrated that body weight increased with time in the control group (108.19 ± 1.31%, n = 7). The increase of mice body weight was significantly less in the EA group (104.41 ± 0.76%, p < 0.05, compared with the control group, n = 7) but not in the sham EA group (109.1 ± 0.63%, p < 0.05, compared with EA group, n = 7). EA did not decrease the gain of body weight in TRPV1 knock mice (107.94 ± 0.41% and 107.79 ± 1.04% for TRPV1(-/-) and TRPV1(-/-) with EA, respectively, p > 0.05). The visceral white adipose tissue (WAT) weight was lower in the EA group at 4 weeks after manipulation. Moreover, the protein levels of TRPV1, pPKA, pPKC, and pERK were increased in the dorsal root ganglion (DRG) and spinal cord (SC) after EA treatment but not in the sham EA and TRPV1(-/-) mice. This study suggests that targeting TRPV1 is beneficial in controlling body weight and TRPV1-associated mechanisms in mice.

No MeSH data available.


Related in: MedlinePlus

Electroacupuncture (EA) at ST36 acupoint attenuated body weight gain but not food and water intake.(A) Body weight gain in control, EA and sham groups. (B) Food intake in control, EA and sham groups. (C) Water intake in control, EA and sham groups. (D) Body weight gain in TRPV1−/− and TRPV1−/− + EA groups. (E) Food intake in TRPV1−/− and TRPV1−/− + EA groups. (E) Water intake in TRPV1−/− and TRPV1−/− + EA groups. Con = control; EA = electroacupuncture; sham = sham-EA group. *p < 0.05 compared to baseline; **p < 0.01 compared to baseline; #p < 0.05 compared to baseline ##p < 0.01 compared to baseline (n = 7 mice per group).
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f1: Electroacupuncture (EA) at ST36 acupoint attenuated body weight gain but not food and water intake.(A) Body weight gain in control, EA and sham groups. (B) Food intake in control, EA and sham groups. (C) Water intake in control, EA and sham groups. (D) Body weight gain in TRPV1−/− and TRPV1−/− + EA groups. (E) Food intake in TRPV1−/− and TRPV1−/− + EA groups. (E) Water intake in TRPV1−/− and TRPV1−/− + EA groups. Con = control; EA = electroacupuncture; sham = sham-EA group. *p < 0.05 compared to baseline; **p < 0.01 compared to baseline; #p < 0.05 compared to baseline ##p < 0.01 compared to baseline (n = 7 mice per group).

Mentions: We investigated whether EA at ST36 acupoint can reduce body weight gain. In the control group, the body weights were increased with time (Fig. 1, 108.19 ± 1.31%, p < 0.05, compared with baseline, n = 7). We further performed EA on normal mice, and the results indicated that EA could reliably reduce the weight gain (Fig. 1, 104.41 ± 0.76% p < 0.05, compared with control group, n = 7). Similar results were not observed if electrical stimulation was employed in non-acupoint gluteal maximus muscle (GM) area supporting an acupoint specificity (Fig. 1, 109.1 ± 0.63%, p < 0.05, compared with the EA group, n = 7). Further, we measured the body weight using TRPV1 knockout mice, which showed a similar increase in body weight as that of the control mice (Fig. 1, 107.94 ± 0.41%, p < 0.05, compared with baseline, n = 7). Importantly, EA cannot reduce body weight gain in TRPV1−/− mice suggesting a crucial target for EA manipulation (Fig. 1, 107.79 ± 1.04%, p < 0.05, compared with TRPV1−/− mice, n = 7).


Targeting TRPV1 for Body Weight Control using TRPV1(-/-) Mice and Electroacupuncture.

Choowanthanapakorn M, Lu KW, Yang J, Hsieh CL, Lin YW - Sci Rep (2015)

Electroacupuncture (EA) at ST36 acupoint attenuated body weight gain but not food and water intake.(A) Body weight gain in control, EA and sham groups. (B) Food intake in control, EA and sham groups. (C) Water intake in control, EA and sham groups. (D) Body weight gain in TRPV1−/− and TRPV1−/− + EA groups. (E) Food intake in TRPV1−/− and TRPV1−/− + EA groups. (E) Water intake in TRPV1−/− and TRPV1−/− + EA groups. Con = control; EA = electroacupuncture; sham = sham-EA group. *p < 0.05 compared to baseline; **p < 0.01 compared to baseline; #p < 0.05 compared to baseline ##p < 0.01 compared to baseline (n = 7 mice per group).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4664894&req=5

f1: Electroacupuncture (EA) at ST36 acupoint attenuated body weight gain but not food and water intake.(A) Body weight gain in control, EA and sham groups. (B) Food intake in control, EA and sham groups. (C) Water intake in control, EA and sham groups. (D) Body weight gain in TRPV1−/− and TRPV1−/− + EA groups. (E) Food intake in TRPV1−/− and TRPV1−/− + EA groups. (E) Water intake in TRPV1−/− and TRPV1−/− + EA groups. Con = control; EA = electroacupuncture; sham = sham-EA group. *p < 0.05 compared to baseline; **p < 0.01 compared to baseline; #p < 0.05 compared to baseline ##p < 0.01 compared to baseline (n = 7 mice per group).
Mentions: We investigated whether EA at ST36 acupoint can reduce body weight gain. In the control group, the body weights were increased with time (Fig. 1, 108.19 ± 1.31%, p < 0.05, compared with baseline, n = 7). We further performed EA on normal mice, and the results indicated that EA could reliably reduce the weight gain (Fig. 1, 104.41 ± 0.76% p < 0.05, compared with control group, n = 7). Similar results were not observed if electrical stimulation was employed in non-acupoint gluteal maximus muscle (GM) area supporting an acupoint specificity (Fig. 1, 109.1 ± 0.63%, p < 0.05, compared with the EA group, n = 7). Further, we measured the body weight using TRPV1 knockout mice, which showed a similar increase in body weight as that of the control mice (Fig. 1, 107.94 ± 0.41%, p < 0.05, compared with baseline, n = 7). Importantly, EA cannot reduce body weight gain in TRPV1−/− mice suggesting a crucial target for EA manipulation (Fig. 1, 107.79 ± 1.04%, p < 0.05, compared with TRPV1−/− mice, n = 7).

Bottom Line: The results demonstrated that body weight increased with time in the control group (108.19 ± 1.31%, n = 7).EA did not decrease the gain of body weight in TRPV1 knock mice (107.94 ± 0.41% and 107.79 ± 1.04% for TRPV1(-/-) and TRPV1(-/-) with EA, respectively, p > 0.05).The visceral white adipose tissue (WAT) weight was lower in the EA group at 4 weeks after manipulation.

View Article: PubMed Central - PubMed

Affiliation: College of Chinese Medicine, Graduate Institute of Acupuncture Science and International Master Program, China Medical University, Taichung 40402, Taiwan.

ABSTRACT
Obesity is a global social medical problem resulting in morbidity as high as 20-30%. Here we investigated whether the manipulation of TRPV1 can control mice body weight through electroacupuncture (EA). The results demonstrated that body weight increased with time in the control group (108.19 ± 1.31%, n = 7). The increase of mice body weight was significantly less in the EA group (104.41 ± 0.76%, p < 0.05, compared with the control group, n = 7) but not in the sham EA group (109.1 ± 0.63%, p < 0.05, compared with EA group, n = 7). EA did not decrease the gain of body weight in TRPV1 knock mice (107.94 ± 0.41% and 107.79 ± 1.04% for TRPV1(-/-) and TRPV1(-/-) with EA, respectively, p > 0.05). The visceral white adipose tissue (WAT) weight was lower in the EA group at 4 weeks after manipulation. Moreover, the protein levels of TRPV1, pPKA, pPKC, and pERK were increased in the dorsal root ganglion (DRG) and spinal cord (SC) after EA treatment but not in the sham EA and TRPV1(-/-) mice. This study suggests that targeting TRPV1 is beneficial in controlling body weight and TRPV1-associated mechanisms in mice.

No MeSH data available.


Related in: MedlinePlus