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Tuning of nanoparticle biological functionality through controlled surface chemistry and characterisation at the bioconjugated nanoparticle surface.

Hristov DR, Rocks L, Kelly PM, Thomas SS, Pitek AS, Verderio P, Mahon E, Dawson KA - Sci Rep (2015)

Bottom Line: We have used a silica - PEG based bionanoconjugate synthetic scheme to study the subtle connection between cell receptor specific recognition and architecture of surface functionalization chemistry.Extensive physicochemical characterization of the grafted architecture is capable of capturing significant levels of detail of both the linker and grafted organization, allowing for improved reproducibility and ultimately insight into biological functionality.Our data suggest that scaffold details, propagating PEG layer architecture effects, determine not only the rate of uptake of conjugated nanoparticles into cells but also, more significantly, the specificity of pathways via which uptake occurs.

View Article: PubMed Central - PubMed

Affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.

ABSTRACT
We have used a silica - PEG based bionanoconjugate synthetic scheme to study the subtle connection between cell receptor specific recognition and architecture of surface functionalization chemistry. Extensive physicochemical characterization of the grafted architecture is capable of capturing significant levels of detail of both the linker and grafted organization, allowing for improved reproducibility and ultimately insight into biological functionality. Our data suggest that scaffold details, propagating PEG layer architecture effects, determine not only the rate of uptake of conjugated nanoparticles into cells but also, more significantly, the specificity of pathways via which uptake occurs.

No MeSH data available.


Cell Biology correlates to PEG layer NMR characteristics.(a). Specific uptake Fraction (mRNA silenced component/total uptake component) for particles with different amine densities (H, M, L and VL) (each point average of more than 5 independent batches) (black line) and average median intensities associated with cellular uptake (blue line). (b). NMR signal parameters for particle series (H, M, L and VL), showing ligand concentration normalized integration (CNI), FWHM intact and FWHM following nanoparticle dissolution.
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f4: Cell Biology correlates to PEG layer NMR characteristics.(a). Specific uptake Fraction (mRNA silenced component/total uptake component) for particles with different amine densities (H, M, L and VL) (each point average of more than 5 independent batches) (black line) and average median intensities associated with cellular uptake (blue line). (b). NMR signal parameters for particle series (H, M, L and VL), showing ligand concentration normalized integration (CNI), FWHM intact and FWHM following nanoparticle dissolution.

Mentions: This binding behaviour mirrors in vitro cell uptake experiments (referred to as “receptor specific frac.” in Fig. 4a). The receptor specific endocytosis of particles was measured by comparing particle uptake into cells with reduced expression of TfR with the uptake into a control population (see supporting section “Cell silencing and flow cytometry” for full details).


Tuning of nanoparticle biological functionality through controlled surface chemistry and characterisation at the bioconjugated nanoparticle surface.

Hristov DR, Rocks L, Kelly PM, Thomas SS, Pitek AS, Verderio P, Mahon E, Dawson KA - Sci Rep (2015)

Cell Biology correlates to PEG layer NMR characteristics.(a). Specific uptake Fraction (mRNA silenced component/total uptake component) for particles with different amine densities (H, M, L and VL) (each point average of more than 5 independent batches) (black line) and average median intensities associated with cellular uptake (blue line). (b). NMR signal parameters for particle series (H, M, L and VL), showing ligand concentration normalized integration (CNI), FWHM intact and FWHM following nanoparticle dissolution.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664868&req=5

f4: Cell Biology correlates to PEG layer NMR characteristics.(a). Specific uptake Fraction (mRNA silenced component/total uptake component) for particles with different amine densities (H, M, L and VL) (each point average of more than 5 independent batches) (black line) and average median intensities associated with cellular uptake (blue line). (b). NMR signal parameters for particle series (H, M, L and VL), showing ligand concentration normalized integration (CNI), FWHM intact and FWHM following nanoparticle dissolution.
Mentions: This binding behaviour mirrors in vitro cell uptake experiments (referred to as “receptor specific frac.” in Fig. 4a). The receptor specific endocytosis of particles was measured by comparing particle uptake into cells with reduced expression of TfR with the uptake into a control population (see supporting section “Cell silencing and flow cytometry” for full details).

Bottom Line: We have used a silica - PEG based bionanoconjugate synthetic scheme to study the subtle connection between cell receptor specific recognition and architecture of surface functionalization chemistry.Extensive physicochemical characterization of the grafted architecture is capable of capturing significant levels of detail of both the linker and grafted organization, allowing for improved reproducibility and ultimately insight into biological functionality.Our data suggest that scaffold details, propagating PEG layer architecture effects, determine not only the rate of uptake of conjugated nanoparticles into cells but also, more significantly, the specificity of pathways via which uptake occurs.

View Article: PubMed Central - PubMed

Affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.

ABSTRACT
We have used a silica - PEG based bionanoconjugate synthetic scheme to study the subtle connection between cell receptor specific recognition and architecture of surface functionalization chemistry. Extensive physicochemical characterization of the grafted architecture is capable of capturing significant levels of detail of both the linker and grafted organization, allowing for improved reproducibility and ultimately insight into biological functionality. Our data suggest that scaffold details, propagating PEG layer architecture effects, determine not only the rate of uptake of conjugated nanoparticles into cells but also, more significantly, the specificity of pathways via which uptake occurs.

No MeSH data available.