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Ergosterol Alleviates Kidney Injury in Streptozotocin-Induced Diabetic Mice.

Ang L, Yuguang L, Liying W, Shuying Z, Liting X, Shumin W - Evid Based Complement Alternat Med (2015)

Bottom Line: The expressions of PI3K, p-PI3K, Akt, p-Akt, NF-κBp65, p-NF-κBp65, IκBα, and p-IκBα were analyzed by western blot.ERG significantly reduced the concentrations of blood glucose, uric acid, creatinine, TG, and TC.Serum insulin was elevated with ERG treatment.

View Article: PubMed Central - PubMed

Affiliation: Changchun University of Traditional Chinese Medicine, Jilin, Changchun 130117, China.

ABSTRACT
Ergosterol (ERG) has been widely used in the development of novel drugs due to its unique physiological function. However, little is known about the protective effects of ERG on diabetes. Hence, the current study was designed to evaluate the positive role of ergosterol on streptozotocin- (STZ-) induced diabetes in mice. Oral glucose tolerance test (OGTT) was carried out to assess blood glucose level. Biochemical parameters such as uric acid, creatinine, serum insulin, triglycerides (TG), and total cholesterol (TC) were also measured. Pathological condition of kidney was examined by hematoxylin-eosin (H&E) staining. The expressions of PI3K, p-PI3K, Akt, p-Akt, NF-κBp65, p-NF-κBp65, IκBα, and p-IκBα were analyzed by western blot. ERG significantly reduced the concentrations of blood glucose, uric acid, creatinine, TG, and TC. Serum insulin was elevated with ERG treatment. In addition, renal pathologic changes of diabetes mice were also alleviated by ERG. Obtained data revealed that ERG restored the levels of PI3K/Akt/NF-κB signaling-related proteins in comparison with diabetes mice. Above all, it could be assumed that ERG might play a positive role in regulating STZ-induced diabetes through suppressing PI3K/Akt/NF-κB pathway.

No MeSH data available.


Related in: MedlinePlus

Effect of ERG on PI3K/Akt/NF-κB pathway-related pathway. Mice were intraperitoneally injected with 135 mg/kg of STZ. ALL (5 mg/kg) and ERG (50, 100 mg/kg) were intragastrically treated for consecutive 28 days. Values are expressed as means ± SDs. Compared with control: ##P < 0.01; compared with model: ∗P < 0.05, ∗∗P < 0.01. Compared with STZ + ERG (50 mg/kg) group: @P < 0.05. A: control; B: STZ; C: STZ + ALL (5 mg/kg); D: STZ + ERG (50 mg/kg); E: STZ + ERG (100 mg/kg).
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fig5: Effect of ERG on PI3K/Akt/NF-κB pathway-related pathway. Mice were intraperitoneally injected with 135 mg/kg of STZ. ALL (5 mg/kg) and ERG (50, 100 mg/kg) were intragastrically treated for consecutive 28 days. Values are expressed as means ± SDs. Compared with control: ##P < 0.01; compared with model: ∗P < 0.05, ∗∗P < 0.01. Compared with STZ + ERG (50 mg/kg) group: @P < 0.05. A: control; B: STZ; C: STZ + ALL (5 mg/kg); D: STZ + ERG (50 mg/kg); E: STZ + ERG (100 mg/kg).

Mentions: To further investigate the possible antidiabetic mechanism, the expressions of PI3K/Akt/NF-κB pathway-related proteins were also detected in renal. As presented in Figure 5, the expressions of p-PI3K, p-Akt, p-NF-κBp65, and p-IκBα were upregulated in diabetes mice, taking the expressions of PI3K, Akt, NF-κBp65, and IκBα as inner controls, respectively, (P < 0.01). On the contrary, these situations were obviously attenuated with the treatment of ERG and ALL (P < 0.01 or P < 0.1). Notably, when compared with the ERG-treated mice, the phosphorylated PI3K (P < 0.1) and NF-κB (P < 0.1) were more effectively downregulated in ALL-treated mice compared with those in ERG (50 mg/kg) treated mice.


Ergosterol Alleviates Kidney Injury in Streptozotocin-Induced Diabetic Mice.

Ang L, Yuguang L, Liying W, Shuying Z, Liting X, Shumin W - Evid Based Complement Alternat Med (2015)

Effect of ERG on PI3K/Akt/NF-κB pathway-related pathway. Mice were intraperitoneally injected with 135 mg/kg of STZ. ALL (5 mg/kg) and ERG (50, 100 mg/kg) were intragastrically treated for consecutive 28 days. Values are expressed as means ± SDs. Compared with control: ##P < 0.01; compared with model: ∗P < 0.05, ∗∗P < 0.01. Compared with STZ + ERG (50 mg/kg) group: @P < 0.05. A: control; B: STZ; C: STZ + ALL (5 mg/kg); D: STZ + ERG (50 mg/kg); E: STZ + ERG (100 mg/kg).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig5: Effect of ERG on PI3K/Akt/NF-κB pathway-related pathway. Mice were intraperitoneally injected with 135 mg/kg of STZ. ALL (5 mg/kg) and ERG (50, 100 mg/kg) were intragastrically treated for consecutive 28 days. Values are expressed as means ± SDs. Compared with control: ##P < 0.01; compared with model: ∗P < 0.05, ∗∗P < 0.01. Compared with STZ + ERG (50 mg/kg) group: @P < 0.05. A: control; B: STZ; C: STZ + ALL (5 mg/kg); D: STZ + ERG (50 mg/kg); E: STZ + ERG (100 mg/kg).
Mentions: To further investigate the possible antidiabetic mechanism, the expressions of PI3K/Akt/NF-κB pathway-related proteins were also detected in renal. As presented in Figure 5, the expressions of p-PI3K, p-Akt, p-NF-κBp65, and p-IκBα were upregulated in diabetes mice, taking the expressions of PI3K, Akt, NF-κBp65, and IκBα as inner controls, respectively, (P < 0.01). On the contrary, these situations were obviously attenuated with the treatment of ERG and ALL (P < 0.01 or P < 0.1). Notably, when compared with the ERG-treated mice, the phosphorylated PI3K (P < 0.1) and NF-κB (P < 0.1) were more effectively downregulated in ALL-treated mice compared with those in ERG (50 mg/kg) treated mice.

Bottom Line: The expressions of PI3K, p-PI3K, Akt, p-Akt, NF-κBp65, p-NF-κBp65, IκBα, and p-IκBα were analyzed by western blot.ERG significantly reduced the concentrations of blood glucose, uric acid, creatinine, TG, and TC.Serum insulin was elevated with ERG treatment.

View Article: PubMed Central - PubMed

Affiliation: Changchun University of Traditional Chinese Medicine, Jilin, Changchun 130117, China.

ABSTRACT
Ergosterol (ERG) has been widely used in the development of novel drugs due to its unique physiological function. However, little is known about the protective effects of ERG on diabetes. Hence, the current study was designed to evaluate the positive role of ergosterol on streptozotocin- (STZ-) induced diabetes in mice. Oral glucose tolerance test (OGTT) was carried out to assess blood glucose level. Biochemical parameters such as uric acid, creatinine, serum insulin, triglycerides (TG), and total cholesterol (TC) were also measured. Pathological condition of kidney was examined by hematoxylin-eosin (H&E) staining. The expressions of PI3K, p-PI3K, Akt, p-Akt, NF-κBp65, p-NF-κBp65, IκBα, and p-IκBα were analyzed by western blot. ERG significantly reduced the concentrations of blood glucose, uric acid, creatinine, TG, and TC. Serum insulin was elevated with ERG treatment. In addition, renal pathologic changes of diabetes mice were also alleviated by ERG. Obtained data revealed that ERG restored the levels of PI3K/Akt/NF-κB signaling-related proteins in comparison with diabetes mice. Above all, it could be assumed that ERG might play a positive role in regulating STZ-induced diabetes through suppressing PI3K/Akt/NF-κB pathway.

No MeSH data available.


Related in: MedlinePlus