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Ergosterol Alleviates Kidney Injury in Streptozotocin-Induced Diabetic Mice.

Ang L, Yuguang L, Liying W, Shuying Z, Liting X, Shumin W - Evid Based Complement Alternat Med (2015)

Bottom Line: The expressions of PI3K, p-PI3K, Akt, p-Akt, NF-κBp65, p-NF-κBp65, IκBα, and p-IκBα were analyzed by western blot.ERG significantly reduced the concentrations of blood glucose, uric acid, creatinine, TG, and TC.Serum insulin was elevated with ERG treatment.

View Article: PubMed Central - PubMed

Affiliation: Changchun University of Traditional Chinese Medicine, Jilin, Changchun 130117, China.

ABSTRACT
Ergosterol (ERG) has been widely used in the development of novel drugs due to its unique physiological function. However, little is known about the protective effects of ERG on diabetes. Hence, the current study was designed to evaluate the positive role of ergosterol on streptozotocin- (STZ-) induced diabetes in mice. Oral glucose tolerance test (OGTT) was carried out to assess blood glucose level. Biochemical parameters such as uric acid, creatinine, serum insulin, triglycerides (TG), and total cholesterol (TC) were also measured. Pathological condition of kidney was examined by hematoxylin-eosin (H&E) staining. The expressions of PI3K, p-PI3K, Akt, p-Akt, NF-κBp65, p-NF-κBp65, IκBα, and p-IκBα were analyzed by western blot. ERG significantly reduced the concentrations of blood glucose, uric acid, creatinine, TG, and TC. Serum insulin was elevated with ERG treatment. In addition, renal pathologic changes of diabetes mice were also alleviated by ERG. Obtained data revealed that ERG restored the levels of PI3K/Akt/NF-κB signaling-related proteins in comparison with diabetes mice. Above all, it could be assumed that ERG might play a positive role in regulating STZ-induced diabetes through suppressing PI3K/Akt/NF-κB pathway.

No MeSH data available.


Related in: MedlinePlus

Effects of ERG on the blood glucose and serum insulin levels. (a) OGTT; (b) serum insulin levels. Mice were intraperitoneally injected with 135 mg/kg of STZ. ALL (5 mg/kg) and ERG (50, 100 mg/kg) were intragastrically treated for consecutive 28 days. Values are expressed as means ± SDs. Compared with control: ##P < 0.01; compared with model: ∗∗P < 0.01.
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fig1: Effects of ERG on the blood glucose and serum insulin levels. (a) OGTT; (b) serum insulin levels. Mice were intraperitoneally injected with 135 mg/kg of STZ. ALL (5 mg/kg) and ERG (50, 100 mg/kg) were intragastrically treated for consecutive 28 days. Values are expressed as means ± SDs. Compared with control: ##P < 0.01; compared with model: ∗∗P < 0.01.

Mentions: As shown in Figure 1, STZ stimulation led to the upregulation of blood glucose (P < 0.01) (Figure 1(a)) and downregulation of serum insulin content (P < 0.01) (Figure 1(b)). However, ERG (50, 100 mg/kg) treatment significantly recovered the abnormal levels and showed the potential to maintain blood glucose balance (P < 0.01). Serum glucose levels presented an apparent peak locating at 90 min. There was little difference at 90 min in the serum glucose between ALL (5 mg/kg) and ERG (50, 100 mg/kg), with ERG (100 mg/kg) showing higher decrease than ERG (50 mg/kg). Meantime, the effect of ALL treatment on the serum insulin level was slightly more pronounced than those of ERG (50, 100 mg/kg) treatments.


Ergosterol Alleviates Kidney Injury in Streptozotocin-Induced Diabetic Mice.

Ang L, Yuguang L, Liying W, Shuying Z, Liting X, Shumin W - Evid Based Complement Alternat Med (2015)

Effects of ERG on the blood glucose and serum insulin levels. (a) OGTT; (b) serum insulin levels. Mice were intraperitoneally injected with 135 mg/kg of STZ. ALL (5 mg/kg) and ERG (50, 100 mg/kg) were intragastrically treated for consecutive 28 days. Values are expressed as means ± SDs. Compared with control: ##P < 0.01; compared with model: ∗∗P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664816&req=5

fig1: Effects of ERG on the blood glucose and serum insulin levels. (a) OGTT; (b) serum insulin levels. Mice were intraperitoneally injected with 135 mg/kg of STZ. ALL (5 mg/kg) and ERG (50, 100 mg/kg) were intragastrically treated for consecutive 28 days. Values are expressed as means ± SDs. Compared with control: ##P < 0.01; compared with model: ∗∗P < 0.01.
Mentions: As shown in Figure 1, STZ stimulation led to the upregulation of blood glucose (P < 0.01) (Figure 1(a)) and downregulation of serum insulin content (P < 0.01) (Figure 1(b)). However, ERG (50, 100 mg/kg) treatment significantly recovered the abnormal levels and showed the potential to maintain blood glucose balance (P < 0.01). Serum glucose levels presented an apparent peak locating at 90 min. There was little difference at 90 min in the serum glucose between ALL (5 mg/kg) and ERG (50, 100 mg/kg), with ERG (100 mg/kg) showing higher decrease than ERG (50 mg/kg). Meantime, the effect of ALL treatment on the serum insulin level was slightly more pronounced than those of ERG (50, 100 mg/kg) treatments.

Bottom Line: The expressions of PI3K, p-PI3K, Akt, p-Akt, NF-κBp65, p-NF-κBp65, IκBα, and p-IκBα were analyzed by western blot.ERG significantly reduced the concentrations of blood glucose, uric acid, creatinine, TG, and TC.Serum insulin was elevated with ERG treatment.

View Article: PubMed Central - PubMed

Affiliation: Changchun University of Traditional Chinese Medicine, Jilin, Changchun 130117, China.

ABSTRACT
Ergosterol (ERG) has been widely used in the development of novel drugs due to its unique physiological function. However, little is known about the protective effects of ERG on diabetes. Hence, the current study was designed to evaluate the positive role of ergosterol on streptozotocin- (STZ-) induced diabetes in mice. Oral glucose tolerance test (OGTT) was carried out to assess blood glucose level. Biochemical parameters such as uric acid, creatinine, serum insulin, triglycerides (TG), and total cholesterol (TC) were also measured. Pathological condition of kidney was examined by hematoxylin-eosin (H&E) staining. The expressions of PI3K, p-PI3K, Akt, p-Akt, NF-κBp65, p-NF-κBp65, IκBα, and p-IκBα were analyzed by western blot. ERG significantly reduced the concentrations of blood glucose, uric acid, creatinine, TG, and TC. Serum insulin was elevated with ERG treatment. In addition, renal pathologic changes of diabetes mice were also alleviated by ERG. Obtained data revealed that ERG restored the levels of PI3K/Akt/NF-κB signaling-related proteins in comparison with diabetes mice. Above all, it could be assumed that ERG might play a positive role in regulating STZ-induced diabetes through suppressing PI3K/Akt/NF-κB pathway.

No MeSH data available.


Related in: MedlinePlus