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Serological Survey for Avian Influenza in Turkeys in Three States of Southwest Nigeria.

Oluwayelu DO, Aiki-Raji CO, Adigun OT, Olofintuyi OK, Adebiyi AI - Influenza Res Treat (2015)

Bottom Line: Of the 23 ELISA-positive samples, 18 were positive for anti-AIV H3N8 antibodies only and four were positive for both anti-AIV H3N8 and H5N2 antibodies indicating a mixed infection, while five were negative for antibodies to either of the two AIV subtypes.We advocate further studies to determine the potential role of turkeys in the zoonotic transmission of AIVs in Nigeria.Additionally, the practice of rearing turkeys with chickens should be discouraged.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Microbiology and Parasitology, University of Ibadan, Ibadan 20005, Nigeria.

ABSTRACT
Since the first outbreak of avian influenza (AI) in Nigeria in 2006, there has been continuous monitoring of the disease in chickens with little attention given to turkeys. As part of on-going surveillance for AI in southwest Nigeria, we used a competitive ELISA to detect anti-AI virus antibodies in 520 turkey sera obtained from poultry farms in Oyo, Osun, and Ondo states while haemagglutination inhibiting antibodies against low pathogenic AI viruses (LPAIVs) were detected using H3N8 and H5N2 subtype-specific antigens. The overall seroprevalence obtained by ELISA was 4.4% (23/520). Of the 23 ELISA-positive samples, 18 were positive for anti-AIV H3N8 antibodies only and four were positive for both anti-AIV H3N8 and H5N2 antibodies indicating a mixed infection, while five were negative for antibodies to either of the two AIV subtypes. Considering that turkeys have been implicated as a mixing vessel for generating influenza virus reassortants of human and avian origin, the detection of antibodies to LPAIV H3N8 and H5N2 in these turkeys is of public health concern. We advocate further studies to determine the potential role of turkeys in the zoonotic transmission of AIVs in Nigeria. Additionally, the practice of rearing turkeys with chickens should be discouraged.

No MeSH data available.


Related in: MedlinePlus

Map of Nigeria showing the study area (A = Oyo; B = Osun; C = Ondo).
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fig1: Map of Nigeria showing the study area (A = Oyo; B = Osun; C = Ondo).

Mentions: A total of 520 (203 males and 317 females) apparently healthy turkeys sampled from 22 different flocks located in Oyo, Osun, and Ondo states, southwest Nigeria (Figure 1), were used for this study. While 315 samples were collected from 13 flocks in Oyo state, 95 were collected from six flocks in Osun state and 110 samples were collected from only three flocks in Ondo state. Additionally, the turkeys comprised 331 local and 189 exotic varieties. The southwest region plays a leading role in poultry production since an estimated 65% of Nigeria's commercial poultry population is concentrated there [25, 29].


Serological Survey for Avian Influenza in Turkeys in Three States of Southwest Nigeria.

Oluwayelu DO, Aiki-Raji CO, Adigun OT, Olofintuyi OK, Adebiyi AI - Influenza Res Treat (2015)

Map of Nigeria showing the study area (A = Oyo; B = Osun; C = Ondo).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664809&req=5

fig1: Map of Nigeria showing the study area (A = Oyo; B = Osun; C = Ondo).
Mentions: A total of 520 (203 males and 317 females) apparently healthy turkeys sampled from 22 different flocks located in Oyo, Osun, and Ondo states, southwest Nigeria (Figure 1), were used for this study. While 315 samples were collected from 13 flocks in Oyo state, 95 were collected from six flocks in Osun state and 110 samples were collected from only three flocks in Ondo state. Additionally, the turkeys comprised 331 local and 189 exotic varieties. The southwest region plays a leading role in poultry production since an estimated 65% of Nigeria's commercial poultry population is concentrated there [25, 29].

Bottom Line: Of the 23 ELISA-positive samples, 18 were positive for anti-AIV H3N8 antibodies only and four were positive for both anti-AIV H3N8 and H5N2 antibodies indicating a mixed infection, while five were negative for antibodies to either of the two AIV subtypes.We advocate further studies to determine the potential role of turkeys in the zoonotic transmission of AIVs in Nigeria.Additionally, the practice of rearing turkeys with chickens should be discouraged.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Microbiology and Parasitology, University of Ibadan, Ibadan 20005, Nigeria.

ABSTRACT
Since the first outbreak of avian influenza (AI) in Nigeria in 2006, there has been continuous monitoring of the disease in chickens with little attention given to turkeys. As part of on-going surveillance for AI in southwest Nigeria, we used a competitive ELISA to detect anti-AI virus antibodies in 520 turkey sera obtained from poultry farms in Oyo, Osun, and Ondo states while haemagglutination inhibiting antibodies against low pathogenic AI viruses (LPAIVs) were detected using H3N8 and H5N2 subtype-specific antigens. The overall seroprevalence obtained by ELISA was 4.4% (23/520). Of the 23 ELISA-positive samples, 18 were positive for anti-AIV H3N8 antibodies only and four were positive for both anti-AIV H3N8 and H5N2 antibodies indicating a mixed infection, while five were negative for antibodies to either of the two AIV subtypes. Considering that turkeys have been implicated as a mixing vessel for generating influenza virus reassortants of human and avian origin, the detection of antibodies to LPAIV H3N8 and H5N2 in these turkeys is of public health concern. We advocate further studies to determine the potential role of turkeys in the zoonotic transmission of AIVs in Nigeria. Additionally, the practice of rearing turkeys with chickens should be discouraged.

No MeSH data available.


Related in: MedlinePlus