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The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus

Intracellular ROS quantification: (a) FL1 histogram plots, showing the distribution of cell count versus DCF fluorescence, and (b) Mean Fluorescence Intensity (MFI) histograms results of HaCaT UV-B irradiated cells, IR (225 mJ/cm2), and nonirradiated cells (NI) exposed to 10 μM complexed lycopene (Lyc-CD) and to the respective controls labelled with DCF-DA. Statistical analysis: One-Way ANOVA with Multiple Pairwise Comparisons: medians with different letters are significantly different (P < 0.05).
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Related In: Results  -  Collection


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fig8: Intracellular ROS quantification: (a) FL1 histogram plots, showing the distribution of cell count versus DCF fluorescence, and (b) Mean Fluorescence Intensity (MFI) histograms results of HaCaT UV-B irradiated cells, IR (225 mJ/cm2), and nonirradiated cells (NI) exposed to 10 μM complexed lycopene (Lyc-CD) and to the respective controls labelled with DCF-DA. Statistical analysis: One-Way ANOVA with Multiple Pairwise Comparisons: medians with different letters are significantly different (P < 0.05).

Mentions: The results of irradiated and nonirradiated cells are represented in Figure 8. UVB irradiation induced an increase in ROS intracellular production. In fact, regarding ROS production, two cell populations were observed in irradiated cells against one in nonirradiated cells. The Median Fluorescence Intensity (MFI) from irradiated samples was statistically higher than MFI from correspondent nonirradiated cells, as theoretically expected. Complexed lycopene did not increase ROS production in nonirradiated cells. However, in irradiated cells Lyc-CD induced a significant ROS increase compared to irradiated nonexposed cells.


The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Intracellular ROS quantification: (a) FL1 histogram plots, showing the distribution of cell count versus DCF fluorescence, and (b) Mean Fluorescence Intensity (MFI) histograms results of HaCaT UV-B irradiated cells, IR (225 mJ/cm2), and nonirradiated cells (NI) exposed to 10 μM complexed lycopene (Lyc-CD) and to the respective controls labelled with DCF-DA. Statistical analysis: One-Way ANOVA with Multiple Pairwise Comparisons: medians with different letters are significantly different (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664803&req=5

fig8: Intracellular ROS quantification: (a) FL1 histogram plots, showing the distribution of cell count versus DCF fluorescence, and (b) Mean Fluorescence Intensity (MFI) histograms results of HaCaT UV-B irradiated cells, IR (225 mJ/cm2), and nonirradiated cells (NI) exposed to 10 μM complexed lycopene (Lyc-CD) and to the respective controls labelled with DCF-DA. Statistical analysis: One-Way ANOVA with Multiple Pairwise Comparisons: medians with different letters are significantly different (P < 0.05).
Mentions: The results of irradiated and nonirradiated cells are represented in Figure 8. UVB irradiation induced an increase in ROS intracellular production. In fact, regarding ROS production, two cell populations were observed in irradiated cells against one in nonirradiated cells. The Median Fluorescence Intensity (MFI) from irradiated samples was statistically higher than MFI from correspondent nonirradiated cells, as theoretically expected. Complexed lycopene did not increase ROS production in nonirradiated cells. However, in irradiated cells Lyc-CD induced a significant ROS increase compared to irradiated nonexposed cells.

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus