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The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus

Representation of Bax, Bcl-2, caspase 3, and TRAIL gene expression values in HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) normalized to the SDHA reference gene. Cells were irradiated with 225 mJ/cm2 UV-B (IR) and nonirradiated (NI). Results are expressed as mean ± SEM of three technical replicates from two independent assays.
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fig7: Representation of Bax, Bcl-2, caspase 3, and TRAIL gene expression values in HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) normalized to the SDHA reference gene. Cells were irradiated with 225 mJ/cm2 UV-B (IR) and nonirradiated (NI). Results are expressed as mean ± SEM of three technical replicates from two independent assays.

Mentions: Under UVB irradiation, lycopene complex exerted proapoptotic effects compared to irradiated but not exposed cells (Figure 7). Compared to irradiated and nonexposed cells, in irradiated cells the exposure to vehicle CD inhibited antiapoptotic BCL2 expression but did not increase proapoptotic BAX expression. Contrarily to this, in irradiated cells Lyc-CD increased proapoptotic BAX expression but did not inhibit antiapoptotic BCL2 expression. This observation points to a proapoptotic effect of Lyc-CD mediated by BAX upregulation. Moreover, in irradiated cells Lyc-CD showed lower caspase 3 gene (CASP3) gene expression compared to nonexposed, irradiated cells; however, it increased CASP3 gene expression comparatively to CD vehicle. TRAIL is a proapoptotic cytokine secreted by many cell types; however, in this study, UVB light was found to decrease TRAIL expression.


The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Representation of Bax, Bcl-2, caspase 3, and TRAIL gene expression values in HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) normalized to the SDHA reference gene. Cells were irradiated with 225 mJ/cm2 UV-B (IR) and nonirradiated (NI). Results are expressed as mean ± SEM of three technical replicates from two independent assays.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664803&req=5

fig7: Representation of Bax, Bcl-2, caspase 3, and TRAIL gene expression values in HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) normalized to the SDHA reference gene. Cells were irradiated with 225 mJ/cm2 UV-B (IR) and nonirradiated (NI). Results are expressed as mean ± SEM of three technical replicates from two independent assays.
Mentions: Under UVB irradiation, lycopene complex exerted proapoptotic effects compared to irradiated but not exposed cells (Figure 7). Compared to irradiated and nonexposed cells, in irradiated cells the exposure to vehicle CD inhibited antiapoptotic BCL2 expression but did not increase proapoptotic BAX expression. Contrarily to this, in irradiated cells Lyc-CD increased proapoptotic BAX expression but did not inhibit antiapoptotic BCL2 expression. This observation points to a proapoptotic effect of Lyc-CD mediated by BAX upregulation. Moreover, in irradiated cells Lyc-CD showed lower caspase 3 gene (CASP3) gene expression compared to nonexposed, irradiated cells; however, it increased CASP3 gene expression comparatively to CD vehicle. TRAIL is a proapoptotic cytokine secreted by many cell types; however, in this study, UVB light was found to decrease TRAIL expression.

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus