Limits...
The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus

Effect of lycopene and UV-B on the cell cycle: (a) cell cycle histograms of UV-B (225 mJ/cm2) irradiated (IR) and nonirradiated (NI) HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) and to the respective controls; (b) cell cycle phase distribution of UV-B (225 mJ/cm2) irradiated (IR) and nonirradiated (NI) HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) and the respective controls, including cyclodextrin vehicle (NI, CD and IR, CD). Results are expressed as percentage (mean ± SD). Statistical analysis: One-Way ANOVA with All Pairwise Multiple Comparison Procedures: means with different letters (A, B, and C) are significantly different (P < 0.05). In this case, only the statistical differences between groups presenting differences (G0/G1 and sub-G1) were marked for simplification purposes. Comparison between % cells with sub-G1 amount of DNA (#) and % cells in G0/G1 phase (∗) is presented.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4664803&req=5

fig5: Effect of lycopene and UV-B on the cell cycle: (a) cell cycle histograms of UV-B (225 mJ/cm2) irradiated (IR) and nonirradiated (NI) HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) and to the respective controls; (b) cell cycle phase distribution of UV-B (225 mJ/cm2) irradiated (IR) and nonirradiated (NI) HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) and the respective controls, including cyclodextrin vehicle (NI, CD and IR, CD). Results are expressed as percentage (mean ± SD). Statistical analysis: One-Way ANOVA with All Pairwise Multiple Comparison Procedures: means with different letters (A, B, and C) are significantly different (P < 0.05). In this case, only the statistical differences between groups presenting differences (G0/G1 and sub-G1) were marked for simplification purposes. Comparison between % cells with sub-G1 amount of DNA (#) and % cells in G0/G1 phase (∗) is presented.

Mentions: Figure 5 shows representative histograms of cell cycle of HaCaT cells after 10 μM complexed lycopene exposure and UV-B irradiation (225 mJ/cm2). Cell cycle analysis shows that complexed lycopene exposure alone did not significantly (P > 0.05) affect the dynamic of cell cycle in comparison to control cells. Compared to nonirradiated and nonexposed cells, irradiation induced a decrease in the percentage of cells in the G0/G1 phase of cell cycle especially in complexed lycopene and CD exposed cells (P = 0.011 and 0.008, resp.) (Figure 5). Although the S and G2 phases were not significantly affected by any of the treatments, an increase in S-phase frequency can be observed.


The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Effect of lycopene and UV-B on the cell cycle: (a) cell cycle histograms of UV-B (225 mJ/cm2) irradiated (IR) and nonirradiated (NI) HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) and to the respective controls; (b) cell cycle phase distribution of UV-B (225 mJ/cm2) irradiated (IR) and nonirradiated (NI) HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) and the respective controls, including cyclodextrin vehicle (NI, CD and IR, CD). Results are expressed as percentage (mean ± SD). Statistical analysis: One-Way ANOVA with All Pairwise Multiple Comparison Procedures: means with different letters (A, B, and C) are significantly different (P < 0.05). In this case, only the statistical differences between groups presenting differences (G0/G1 and sub-G1) were marked for simplification purposes. Comparison between % cells with sub-G1 amount of DNA (#) and % cells in G0/G1 phase (∗) is presented.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664803&req=5

fig5: Effect of lycopene and UV-B on the cell cycle: (a) cell cycle histograms of UV-B (225 mJ/cm2) irradiated (IR) and nonirradiated (NI) HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) and to the respective controls; (b) cell cycle phase distribution of UV-B (225 mJ/cm2) irradiated (IR) and nonirradiated (NI) HaCaT cells exposed to 10 μM complexed lycopene (Lyc-CD) and the respective controls, including cyclodextrin vehicle (NI, CD and IR, CD). Results are expressed as percentage (mean ± SD). Statistical analysis: One-Way ANOVA with All Pairwise Multiple Comparison Procedures: means with different letters (A, B, and C) are significantly different (P < 0.05). In this case, only the statistical differences between groups presenting differences (G0/G1 and sub-G1) were marked for simplification purposes. Comparison between % cells with sub-G1 amount of DNA (#) and % cells in G0/G1 phase (∗) is presented.
Mentions: Figure 5 shows representative histograms of cell cycle of HaCaT cells after 10 μM complexed lycopene exposure and UV-B irradiation (225 mJ/cm2). Cell cycle analysis shows that complexed lycopene exposure alone did not significantly (P > 0.05) affect the dynamic of cell cycle in comparison to control cells. Compared to nonirradiated and nonexposed cells, irradiation induced a decrease in the percentage of cells in the G0/G1 phase of cell cycle especially in complexed lycopene and CD exposed cells (P = 0.011 and 0.008, resp.) (Figure 5). Although the S and G2 phases were not significantly affected by any of the treatments, an increase in S-phase frequency can be observed.

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus